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Assembly of protein S and C4b-binding protein on membranes

The interaction of protein S with membranes and subsequent combination with complement C4b-binding protein (C4BP) was studied. Protein S interacted with phospholipid vesicles in a calcium-dependent manner typical of other vitamin K-dependent proteins. Association of C4BP with protein S showed no apparent selectivity for membrane-bound or solution phase protein S. When bound to the membrane, the pr

Genes for C4b-binding protein alpha- and beta-chains (C4BPA and C4BPB) are located on chromosome 1, band 1q32, in humans and on chromosome 13 in rats

C4b-binding protein is involved in the regulation of the complement system. It is a multimeric protein composed of seven identical alpha-chains and a single copy of a unique beta-chain. The latter was identified only recently and its structure determined by cDNA cloning. Both subunits in C4b-binding protein belong to the same superfamily of proteins composed predominantly of tandemly arranged shor

Human genes for the alpha and beta chains of complement C4b-binding protein are closely linked in a head-to-tail arrangement

C4b-binding protein (C4BP) is an important component in the regulation of the complement system and also binds the anticoagulant vitamin K-dependent protein S. These activities are performed by distinct, although structurally related, polypeptides of 70 kDa (alpha chain) and 45 kDa (beta chain), respectively. In this report we have investigated the genetic relationships between these polypeptides.

Cloning of cDNA coding for the beta chain of human complement component C4b-binding protein : sequence homology with the alpha chain

The major form of complement component C4b-binding protein, a regulator of the complement system, is composed of seven identical 70-kDa alpha chains, each containing a binding site for the complement protein C4b. We recently showed that C4b-binding protein also contains a unique 45-kDa beta chain. It is disulfide-linked to the central core and contains a binding site for the vitamin K-dependent pr

Protein S binding in relation to the subunit composition of human C4b-binding protein

The human regulatory complement component C4b-binding protein (C4BP) circulates in plasma either as a free protein or in a bimolecular complex with the vitamin K-dependent protein S. The major form of C4BP is composed of 7 identical, disulfide-linked 70 kDa subunits (alpha-chains), the arrangement of which gives the C4BP molecule a spider-like appearance. Recently, we identified a unique 45 kDa su

Novel subunit in C4b-binding protein required for protein S binding

C4b-binding protein (C4BP) is a multimeric protein with regulatory functions in the complement system. It also interacts with vitamin K-dependent protein S, which is involved in the regulation of the coagulation system. It has been demonstrated that C4BP consists of seven disulfide-linked, identical 70-kDa subunits, which are arranged to give the molecule a spider-like structure. We now have evide

The protein S-binding site localized to the central core of C4b-binding protein

Human C4b-binding protein (C4BP) is a regulator of the classical pathway of the complement system. It appears in two forms in plasma, as free protein and in a noncovalent complex with the vitamin K-dependent coagulation protein, protein S. In the electron microscope C4BP has a spider-like structure with a central core and seven extended tentacles, each of which has a binding site for C4b, although

Urban Ecosystem Services and Tourism

Urban tourism depends on the place specific qualities of destinations. In many cities, climate change poses a threat to these qualities, through increasing risk of excessive heat, draught and flooding. Cities need to adapt to reduce these risks. One way of doing this is to improve their green infrastructure. Urban forests, parks, rivers and wetlands may help reduce the effects of climate change in

Adaptation and Ecosystem Services in Urban Parks, examples from Berlin

Urban tourism depends on the place specific qualities of destinations. In many cities, climate change poses a threat to these qualities, through increasing risk of excessive heat, draught and flooding. Cities need to adapt to reduce these risks. One way of doing this is to improve their green infrastructure. Urban forests, parks, rivers and wetlands may help reduce the effects of climate change in

More efficient reversal of dabigatran inhibition of coagulation by activated prothrombin complex concentrate or recombinant factor VIIa than by four-factor prothrombin complex concentrate

The number of patients on antithrombotic treatment due to atrial fibrillation and venous thromboembolism is increasing fast due to an aging population. A growing proportion will be treated with novel oral anticoagulants, the first in clinical use was the direct oral thrombin inhibitor dabigatran (Pradaxa®). A small percentage of the patients on dabigatran will experience serious bleeding or be in

Assay discrepancy in mild haemophilia A

UNLABELLED: There are three main methods used to assay factor VIII (FVIII) activity: the one-stage and two-stage clotting assays and the two-stage chromogenic method. The most commonly used assay for the diagnosis of haemophilia A is the automated one-stage FVIII assay. The classical two-stage FVIII assays are less frequently used. The chromogenic FVIII:C assay is a variant of the two-stage assay.

Measuring Oral Direct Inhibitors (ODIs) of thrombin and factor Xa : A recommendation from the Subcommittee on Control of Anticoagulation of the Scientific and Standardisation Committee of the International Society on Thrombosis and Haemostasis

Oral direct inhibitors (ODIs) of thrombin and factor Xa are now approved as anticoagulant drugs. The first two drugs to complete phase III clinical trials for thromboprophylaxis in orthopaedic surgery and treatment of patients with atrial fibrillation or venous thromboembolism were dabigatran and rivaroxaban. These small molecules are given at fixed dose with no requirement for monitoring as pharm

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The article takes its point of departure in a contemporary Scandinavian literary debate regarding the function and possible societal impact of climate-oriented poetry and prose. Against the ecocentric, anti-modernist and anti-formalist tenets dominating this debate, Friis and Thomsen want to examine how the formal aspects of literary texts might be relevant for ecocritical work. Through critical d

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This article discusses the motif of reproductive work as it pops up in Scandinavian poetry in the 1960s and 70s. Shopping for groceries, the peeling and cooking of potatoes and the like are activities that the works of for instance Inger Christensen, Kirsten Thorup, Vita Andersen and Sonja Åkesson mark with a conspicuous poetic intensity that revaluates domestic/reproductive work as work proper.

Combined effect of factor V Leiden and prothrombin 20210A on the risk of venous thromboembolism : Pooled analysis of 8 case-control studies including 2310 cases and 3204 controls

Factor V Leiden and factor II G20210A mutations are two frequent genetic risk factors involved in venous thromboembolism (VTE). The goal of this pooled analysis of 8 case-control studies, comprising a total of 2310 cases and 3204 controls, was to precisely estimate the risk of VTE in patients bearing both mutations (double heterozygotes). Odds ratios for VTE were 4.9 (95% CI; 4.1-5.9) for the fact