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Preparation and crystallization of a Bacillus subtilis arsenate reductase

Arsenate reductase (AR) in B. subtilis is encoded by the chromosomal arsC gene. Together with arsB and arsR, arsC participates in detoxification processes for the arsenate and arsenite ions. Full-length arsenate reductase without any modification has been expressed in Escherichia coli and purified in a soluble form. The recombinant protein has been crystallized at 277 K using polyethyleneglycol (P

The distal heme center in Bacillus subtilis succinate:menaquinone reductase is crucial for electron transfer to menaquinone

Succinate:quinone reductases are membrane-bound enzymes that catalyze electron transfer from succinate to quinone. Some enzymes in vivo reduce ubiquinone (exergonic reaction) whereas others reduce menaquinone (endergonic reaction). The succinate:menaquinone reductases all contain two heme groups in the membrane anchor of the enzyme: a proximal heme (heme b(P)) located close to the negative side of

Efficient spore synthesis in Bacillus subtilis depends on the CcdA protein.

CcdA is known to be required for the synthesis of c-type cytochromes in Bacillus subtilis, but the exact function of this membrane protein is not known. We show that CcdA also plays a role in spore synthesis. The expression of ccdA and the two downstream genes yneI and yneJ was analyzed. There is a promoter for each gene, but there is only one transcription terminator, located after the yneJ gene.

Genes required for cytochrome c synthesis in Bacillus subtilis

Cytochromes of c-type contain covalently bound haem and in bacteria are located on the periplasmic side of the cytoplasmic membrane. More than eight different gene products have been identified as being specifically required for the synthesis of cytochromes c in Gram-negative bacteria. Corresponding genes are not found in the genome sequences of Gram-positive bacteria. Using two random mutagenesis

The Bacillus subtilis ctaB paralogue, yjdK, can complement the heme A synthesis deficiency of a CtaB-deficient mutant

Heme A is a prosthetic group in many respiratory oxidases. It is synthesised from heme B (protoheme IX) with heme O as an intermediate. In Bacillus subtilis two genes required for heme A synthesis, ctaA and ctaB, have been identified. CtaB is the heme O synthase and CtaA is involved in the conversion of heme O to heme A. A ctaB paralogue, yjdK, has been identified through the B. subtilis genome se

Fostering innovation : Factors that attract and retain third party developers in mobile ecosystems

The popularity of smartphones and the related growth of mobile application markets created a need for mobile platform owners to open their software platforms up to third party developers in order to meet user demand for mobile applications. This external innovation provides a tremendous opportunity for mobile platform owners to develop a volume and diversity of products they could not develop in-h

Ecosystem Changes in the Automotive Industry : Electric Vehicles and the Increased Importance of Networked Business Models

Platform ecosystems are becoming the locus of innovation-driven economies as technology-based platform within the ecosystem acts as the foundation for innovative product and services developed by the networked business community around that platform. At its core, a technology platform shows architectural properties, which once emerged in an industry could play as the de facto template for defining

An empirical analysis of the openness dimension of OCPP standard

Open Charge Point Protocol (OCPP) is a widely used open communication protocol between a charging station and its managing network system. The central promising idea of the OCPP is that it is an open standard, which provides a standardized communication language with the flexibility of giving charging station owners right to switch network providers at their own will. This paper investigates the o

Enabling Organizational Agility Through Self-Service Business Intelligence: the case of a digital marketplace

Many organizations are adopting business intelligence and analytics systems in order to cope with the increasing digitalization of data intensive environments. In this paper, we study the role of self-service business intelligence (SSBI), a certain capability provided by a business intelligence system, in enabling organizational agility. In particular, the research question we address is as follow

Search for minimal supersymmetric standard model Higgs Bosons H / A and for a Z′ boson in the ττ final state produced in pp collisions at √s=13 TeV with the ATLAS detector

A search for neutral Higgs bosons of the minimal supersymmetric standard model (MSSM) and for a heavneutral Z′ boson is performed using a data sample corresponding to an integrated luminosity of 3.2 fb- 1 from proton–proton collisions at s=13 TeV recorded by the ATLAS detector at the LHC. The heavy resonance is assumed to decay to a τ+τ- pair with at least one τ lepton decaying to final states wit

Performance of pile-up mitigation techniques for jets in pp collisions at √s=8 TeV using the ATLAS detector

The large rate of multiple simultaneous proton–proton interactions, or pile-up, generated by the Large Hadron Collider in Run 1 required the development of many new techniques to mitigate the adverse effects of these conditions. This paper describes the methods employed in the ATLAS experiment to correct for the impact of pile-up on jet energy and jet shapes, and for the presence of spurious addit

Search for new resonances in events with one lepton and missing transverse momentum in pp collisions at s=13 TeV with the ATLAS detector

A search for W′bosons in events with one lepton (electron or muon) and missing transverse momentum is presented. The search uses 3.2 fb−1of pp collision data collected at s=13 TeV by the ATLAS experiment at the LHC in 2015. The transverse mass distribution is examined and no significant excess of events above the level expected from Standard Model processes is observed. Upper limits on the W′boson

Jet-like correlations with neutral pion triggers in pp and central Pb–Pb collisions at 2.76 TeV

We present measurements of two-particle correlations with neutral pion trigger particles of transverse momenta 8>pT trig>16 GeV/c and associated charged particles of 0.5>pT assoc>10 GeV/c versus the azimuthal angle difference Δφ at midrapidity in pp and central Pb–Pb collisions at sNN=2.76 TeV with ALICE. The new measurements exploit associated charged hadrons down to 0.5 GeV/c, which significantl

Search for dark matter produced in association with a hadronically decaying vector boson in pp collisions at √s = 13 TeV with the ATLAS detector

A search is presented for dark matter produced in association with a hadronically decaying W or Z boson using 3.2 fb-1 of pp collisions at √ s = 13 TeV recorded by the ATLAS detector at the Large Hadron Collider. Events with a hadronic jet compatible with a W or Z boson and with large missing transverse momentum are analysed. The data are consistent with the Standard Model predictions and are inte