To study the biochemistry of processing of a soluble protein Ag by an APC, we investigated how 125 I-labeled human insulin (HI) is processed in situ by TA3 mouse hybridoma B cells. Fractionation of TA3 cells into their extracellular, plasma membrane-associated and intracellular compartments coupled with the use of HPLC enabled us to analyze several peptides derived from each compartment. One HI

There is some evidence that housing accessibility, external housing-related control beliefs (HCB) and activities of daily living (ADL) are associated in complex ways; however, these pathways have not been explored in younger old. The aim was to assess the role of external HCB in the relationship between housing accessibility and ADL by applying moderation and mediation models. This was a cross-sec

The second-generation thrombopoietin (TPO) receptor agonists eltrombopag and romiplostim are potent activators of megakaryopoiesis and represent a growing treatment option for patients with thrombocytopenic hematological disorders. Both TPO receptor agonists have been approved worldwide for the treatment of children and adults with chronic immune thrombocytopenia. In the EU and USA, eltrombopag is

The exact mechanism of action of IVIg in the amelioration of immune thrombocytopenic purpura (ITP) is still unclear. Studies have suggested that IVIg may function through the regulation of cytokines, including interleukin-1 receptor antagonist (IL-1Ra), an inhibitor of phagocytosis. Using a mouse model relevant to ITP, we confirm an increase in mouse serum levels of IL-1Ra after exposure to IVIg,

Clinical studies have convincingly demonstrated that WBC reduction of blood components can reduce the incidence of primary HLA antigen alloimmunization at least in patients with acute myeloid leukemia.1 However, despite receiving WBC‐reduced platelets, approximately 19 percent of acute myeloid leukemia patients still became alloimmunized.1 In addition, it is not known whether WBC reduction will pr

Immunologically insulin resistant (IIR) type I diabetic patients possess significantly elevated levels of anti-insulin serum autoantibodies. We investigated whether altered insulin processing by B lymphocytes contributes to this form of insulin resistance. A comparison was made of the 125I-labelled human insulin (HI) peptides processed by Epstein-Barr virus (EBV)-transformed B lymphocytes derived

Objective: A large number of different types of digital innovations for health and medical care have been developed in Sweden under the concept of eHealth. Employees’ interest is of great importance in determining the success of different new digital interventions and technologies. Purpose: In this study, we aimed to elucidate the nurses’ experiences as a first “gatekeeper” in an Internet-based Di

This chapter discusses the changes in protein phosphorylation following ischemia, with particular reference to the regulation of the initiation of protein synthesis. Transient cerebral ischemia seems to induce a post-ischemic imbalance between protein kinase and protein phosphatase activities, leading to a net dephosphorylation of proteins in the vulnerable neurons. This imbalance may lead to the

Leukoreduced allogeneic platelet transfusions have been previously shown to initially stimulate an in vitro cellular cytotoxicity and subsequently induce the formation of immunoglobulin G (IgG) antidonor alloantibodies. To further characterize these responses and determine if they are related, recipient BALB/c H-2(d) mice were treated with aminoguanidine (AMG), a selective inhibitor of inducible n

Presentation of a protein antigen to T cells generally requires that the antigen be enzymatically processed into an immunogenic peptide(s). The identification of a protease(s) and its mechanism of action in the proteolysis of such an antigen is therefore a primary goal in the study of antigen processing. We show here that Insulin degrading enzyme (IDE), a neutral thiol metalloendo-proteinase that

An Escherichia coli strain transfected with a plasmid containing four linked human proinsulin genes was grown in the presence of 35S and 3H labelled amino acids to gain access to human insulin that was radiolabelled at 19 evenly distributed sites throughout the amino acid sequence. The multi-proinsulin precursor was cleaved at methionine residues with cyanogen bromide, then the individual proinsul

With the advancement of the next generation of cellular systems, flexible mechanisms for Discontinuous Reception (DRX) are needed in order to save energy. 5G will bring heterogeneous packet sizes and traffic types, as well as an increasing need for energy efficiency. The current static DRX mechanism is inadequate to meet these needs. In this paper we exploit channel prediction to develop integer p

Nerves containing noradrenaline were studied by formaldehyde-induced fluorescence and neuropeptide Y (NPY) was visualised by immunohistochemistry in the human ovary, Fallopian tube and uterus. All structures were richly supplied with noradrenergic fibres closely associated with the vascular and non-vascular smooth musculature. NPY-containing nerve terminals were consistently fewer, particularly in

1. The effects of 5-hydroxytryptamine (5-HT) on contraction and release of [3H]-noradrenaline were investigated in vitro in bovine ovarian follicle strips. Using available selective agonists and antagonists, an effort was made to characterize the type of receptor mediating the inhibitory effect of 5-HT on neurogenic contraction and release of [3H]-noradrenaline by electrical field stimulation. 2.

The abiding interest of researchers in the nature of mediated scandals continues to provoke discussions of what this phenomenon actually is, and how it is best researched empirically. This article argues that despite the claims that a modern scandal is manifested mainly through traditional and digital media, a careful analysis of the lived experience of this phenomenon—using in-depth qualitative i

Adverse conditions in childhood manifest in a risk for long-term illness and poor labour market performance in the future and add to future health problems. Children and young people in need of care are particularly vulnerable as their future depends on their parents and family and the legal system as well as on social welfare. By identifying their needs we can develop new and relevant knowledge a

The early design stage of a building is decisive for describing the concept of the HVAC system. Designers and practitioners can adjust and optimize the design during this stage as it provides them with enough resilience to adapt new changes. In practice, a well-defined optimization process is essentially required in order to achieve the project’s goals within a reasonable time span. These goals va