Structure of murine complement component C3. I. Nucleotide sequence of cloned complementary and genomic DNA coding for the beta chain
The nucleotide sequence coding for the beta chain of murine C3 was determined from cloned cDNA and genomic DNA fragments. Sonicated subfragments were randomly inserted into the bacteriophage M13 and sequenced using the dideoxynucleotide technique. Each nucleotide was sequenced on average six times in these studies. The derived amino acid sequence includes a signal peptide and a tetra-arginine sequ