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Analysis by high-performance liquid chromatography of radioactively labeled carbohydrate components of proteoglycans

Methods were developed for the separation of radioactively labeled carbohydrate components of proteoglycans by isocratic ion-moderated partition HPLC. Neutral sugars were separated after hydrolysis in trifluoroacetic acid with baseline separation between glucose, xylose, galactose, fucose, and mannose. N-Acetylneuraminic acid, N-acetylated hexosamines, glucose, galactose, and xylitol were likewise

Structural relationship between α1-microglobulin from man, guinea-pig, rat and rabbit

Rabbit α1-microglobulin was purified from the urine of sodium-chromate-treated animals by the use of gel chromatography on Sephadex G-100 affinity chromatography on concanavalin-A - Sepharose and ion-exchange chromatography on DEAE-Sephadex. Rabbit α1-microglobulin had a molecular mass of 25.6 kDa on SDS/polyacrylamide gel electrophoresis. α1-microglobulin has previously been purified from the uri

Rabbit bone matrix induces bone formation in the athymic rat

Rabbit and rat bone matrix were implanted in athymic rat muscle, and the bone yield was measured as total calcium content after 4, 6, and 8 weeks. Matrix from both species induced equal amounts of new bone in the athymic rat. In rabbit and normal rat, the xenogenic matrix induced little or no bone formation. Thus, in the case of rabbit and rat, bone induction is species specific due to immunogenic

Failure of bone induction by bone matrix in adult monkeys

Extraskeletal bone formation can be induced in rodents by implantation of demineralised bone matrix and such implantation has been used to treat bone defects in man, but it is uncertain if induction or merely conduction occurs. We studied bone induction in primates by excising segments of the fibulae of adult squirrel monkeys, defatting and demineralising them before reimplanting them into the qua

Proteoglycans of joint cartilage. Structure, function, turnover and role as markers of joint disease

Joint cartilage consists of cells embedded in a matrix of fibrous collagen within a concentrated water-proteoglycan gel. The integrity of this matrix is crucial for the biomechanical properties of the joint cartilage. The different components of the matrix are synthesized and degraded by the cartilage cells, a process regulated by the amount of mechanical stress applied to the chondrocytes as well

Transient synovitis of the hip in the child : Increased levels of proteoglycan fragments in joint fluid

The levels of proteoglycan antigen were measured in joint aspirates from the hip of children with transient synovitis, septic arthritis, Legg-Calve-Perthes' disease and congenital and traumatic dislocation. Significantly increased levels were found in children with transient synovitis and septic arthritis as compared with other conditions. We propose that the proteoglycan antigens in the joint flu

Drug test chamber : a titanium implant for administration of biochemical agents to a standardized bone callus in situ

A titanium implant in which a conduit is gradually filled with ingrowing bone (the Bone Harvest Chamber) has been modified to allow continuous local treatment of the conduit tissue with biochemical agents. Implants were inserted bilaterally in rabbit tibiae. The tissue content of the bone ingrowth conduits was studied with histology, 99mTc-MDP scintimetry and measurements of total calcium content.

Continuous wave measurements in a network of transducers for structural health monitoring of a large concrete floor slab

Local, superficial damage was detected and localized on an 8 × 2-m concrete floor slab using a structural health monitoring system. A total of 30 piezoelectric transducers, placed in a grid, transmitted and received continuous ultrasonic waves that were measured using a lock-in amplifier. Tomography was used to create images from the measured amplitude and phase of the continuous waves between all

High-pressure transformations of NbO2F

The ReO3-type structure NbO2F, niobium dioxyfluoride, has been studied at high pressures using diamond anvil cells and synchrotron X-ray radiation. High-pressure powder diffraction measurements have been performed up to 40.1 GPa. A phase transition from the cubic (Pm3m) ambient pressure structure to a rhombohedral (R3c) structure at 0.47 GPa has been observed. Rietveld refinements at 1.38, 1.96, 3

Optically detected extended X-ray absorption fine structure study of InGaN/GaN single quantum wells

We have investigated the local atomic environment of the Ga atoms in an InxGa1-xN single quantum well structure using Optically Detected Extended X-ray Absorption Fine Structure (OD-EXAFS). A comparison of the OD-EXAFS data with a theoretical model shows the technique to be site selective for this particular structure and reveals that the quantum well emission originates from regions with x=0.15.

An Evacuation Decision Model based on perceived risk, social influence and behavioural uncertainty

The behaviour of people in the first stage of an evacuation can have a significant impact on the time required to reach a safe place. This behaviour is known in literature as pre-evacuation behaviour and it has been studied for many different evacuating scenarios. Despite the large number of studies, the representation of this behaviour is often oversimplified in most of the existing evacuation mo

Λ/K0s Associated with a Jet in Central Pb–Pb Collisions at √sNN = 2.76 TeV Measured with the ALICE Detector

Denna studie handlar om att experimentellt karakterisera ett materietillstånd som existerade under de första miljondelarna av en sekund under Big Bang, som vi kallar för Kvark-Gluon-Plasma. Med stora partikelacceleratorer som Large Hadron Collider (LHC) vid CERN, kan vi genom kärnkollisioner med mycket hög energi nå så pass extrema temperaturer och densiteter att vi kan återskapa och studera dettaIn high energy heavy ion collisions, the QCD matter undergoes a phase transition to a hot and dense strongly coupled Quark Gluon Plasma, where quarks and gluons are deconfined in a volume of nuclear dimensions. At intermediate pT, 2 < p T < 8 GeV/c, a decoupling from pure hydrodynamical flow is observed, most noticeable in central collisions, demonstrated by the peak in the ratio of baryons to mes

What Became of the New Public Diplomacy? : Recent Developments in British, U.S. & Swedish Public Diplomacy Policy and Evaluation Methods

This article examines the relationship between theories of the ‘new’ public diplomacy and recent attemptsby foreign ministries in the United Kingdom, United States and Sweden to develop public diplomacystrategies for the early twenty-first century. It provides a summary of policy debates in each nation alongsideanalysis of the evaluation methods that have been designed to support them. The article

American Strategic Communication in Iraq : the “Rapid Reaction Media Team”

The purpose of this paper is to interpret an American military media strategy designed for the Iraq war from a perspective drawing on recent theoretical discussions of space and time. The material consists of a short white paper that was declassified under the Freedom of Information Act and published by the NSA in 2007. It outlines a ‘Rapid Reaction Media Team’ which was tasked with designing and