Traditionally, human embryonic stem cells (hESCs) are propagated by mechanical dissection or enzymatic dissociation into clusters of cells. To facilitate up-scaling and the use of hESC in various experimental manipulations, such as fluorescence-activated cell sorting, electroporation, and clonal selection, it is important to develop new, stable culture systems based on single-cell enzymatic propag

AIMS: Although the perpetuation of several supraventricular arrhythmias is critically dependent upon intra-atrial conduction, the literature lacks detailed information on normal values of conduction velocity and degree of anisotropy. In order to explore these factors further, we have measured conduction velocities at the right atrial free wall during sinus rhythm and during atrial pacing in four d

The calpain-10 gene (CAPN10) has been associated with type 2 diabetes, but information on molecular and physiological mechanisms explaining this association is limited. Here we addressed this question by studying the role of CAPN10 for phenotypes associated with type 2 diabetes and free fatty acid (FFA) metabolism. Among 395 type 2 diabetic patients and 298 nondiabetic control subjects from Finlan

Popular Abstract in Swedish Matematiska modeller och simulering är viktiga verktyg för att hantera tekniska system. Trenden idag är att öka komplexiteten i system genom integrerad produktion och processoptimering. Att hantera dessa komplexa system kräver kunskaper inom många områden samtidigt. Modellbibliotek är ett bra sätt att paketera kunskap. Kommersiella simuleringsprogram tillandahåller bra This thesis describes basic models for thermo-fluid systems and the implementation of the models using a flexible object-oriented structure in the ThermoFluid library. Model libraries provide an excellent way to package engineering knowledge of systems to be reused by non-experts. Many commercial simulation packages provide good model libraries, but they are usually domain specific and closed. Mod

Vascular smooth muscle cell migration, occurring after intimal injury, is a substantial clinical problem in atherosclerosis and restenosis after stenting. Here we investigate the effects of polyamine synthesis inhibition on vascular smooth muscle cell migration after maximal and submaximal growth stimulation with PDGF-AB or FCS. Vascular smooth muscle cells were obtained from mouse aorta explants.

The use of surface plasmon resonance (SPR), for the comparison of metal binding properties of polyhistidine tags, was evaluated. Six different tags containing various number of histidines, either none (tags n and t), three (tags H(3)A(3) and H(2)AHA(2)H) or six (tags H-6 and HiS(6)) were genetically fused to the N-terminal of lactate dehydrogenase (LDH). The binding ability of these constructs to

In this paper, we propose a method for suppressing radio frequency interference (RFI) in discrete multitone (DMT) based very high bit rate digital subscriber line (VDSL) systems. The method operates in the frequency domain of a DMT system. First, we derive a model of how an unknown narrow-band RF signal is mapped onto the DMT carriers. Then, by measuring the RFI on a few unused DMT carriers we are

Results from the design of a high-pressure chamber and its components, recently developed at the Lund Institute of Technology, are presented. The results show that at high pressures, when the lubricants have solidified, the density varies linearly with the pressure and the shear strength also increases nearly linearly with pressure. This is also true for the investigated gasses when they are compr

A low molecular weight heparin (LMW-heparin) with a mean molecular weight of 4900 dalton was prepared by controlled enzymatic depolymerization of conventional porcine mucosal heparin. The effects of 2,500, 5,000 and 10,000 U (XaI; 29, 58 and 116 mg) on factor Xa inhibition (XaI), factor IIa inhibition (IIaI), APTT, AT III and platelet count were compared to those of 5,000 U (XaI; 26 mg) of convent

Software inspection is a method to detect faults in the early phases of the software life cycle. In order to estimate the number of faults not found, capture-recapture was introduced for software inspections in 1992 to estimate remaining faults after an inspection. Since then, several papers have been written in the area, concerning the basic theory, evaluation of models and application of the met

Large neutron excess poses new issues concerning the competition of deformation and pairing as well as changes in nuclear shell structure.

The mechanism of thermally induced oxidation of Fe2+ from natural pyrope has been studied at 1000 and 1100 degreesC using Fe-57 Mossbauer spectroscopy in conjunction with XRD, XRF, AFM, QELS, TG, DTA and electron microprobe analyses. At 1000 degreesC, the non-destructive oxidation of Fe2+ in air includes the partial stabilization of Fe3+ in the dodecahedral 24c position of the garnet structure and

Psychiatric casualties among soldiers constitute an inevitable part of modem warfare. For the past hundred years Medicine and Society have shown a growing interest in the combat reactions. The focus has shifted from an organic one towards a psychological and psychiatric perspective. Cultural, social, economical and political forces have influenced the scientific development from the Shell shock to

This article outlines laboratory and analytical studies of salt frost scaling and internal frost resistance of Self-Compacting Concrete, SCC, that contains increased amount of filler, different air content and dissimilar methods of casting. The result were compared with the corresponding properties of normal concrete, NC, with the same water-cement ratio, w/c = 0,39 and air content, 6%. Both 28 an

Filmrecension av Kathryn Bigelows Vikten av vatten (The Weight of Water).

A novel multilayer mirror was designed and fabricated based on a recently developed three-material technology aimed both at reaching reflectivities of about 20% and at controlling dispersion over a bandwidth covering photon energies between 35 and 50 eV. The spectral phase upon reflection was retrieved by measuring interferences in a two-color ionization process using high-order harmonics produced

During biosynthesis of chlorophyll, Mg2+ is inserted into protoporphyrin IX by magnesium chelatase. This enzyme consists of three different subunits of approximate to 40, 70 and 140 kDa. Seven barley mutants deficient in the 40 kDa magnesium chelatase subunit were analysed and it was found that this subunit is essential for the maintenance of the 70 kDa subunit, but not the 140 kDa subunit. The 40