An integrated protein microcharacterization/identification platform has been developed. The system has been designed to allow a high flexibility in order to tackle challenging analytical problems. The platform comprises a cooled microautosampler, an integrated system for microcolumn HPLC, and a capillary reversed-phase column that is interfaced to matrix-assisted laser desorption ionization time-o

This paper presents a picoliter sample preparation technique utilizing the flow-through principle, allowing on-line coupling of chromatographic systems to be made. The work was performed in order to investigate the characteristics and the physicochemical properties of the sample preparation using typical mobile phase conditions from μ-CLC (column liquid chromatography) separations. The device pres

A coupled-column liquid chromatographic system using solid phase extraction pre-columns for the seven chlorotriazines, atrazine, desethylatrazine, desisopropylatrazine (DIA), propazine, simazine, desethylterbuthylazine and terbuthylazine, using photodiode-array detection (PDAD) was developed. Restricted access (RA), was investigated as a pre- column material, making use of non-adsorptive size excl

Molecularly imprinted polymers (MIPs) have been developed mostly for the specific binding of the target, mimicking the function of antibodies. In addition to these " plastic antibodies," completely synthetic catalysts have been created that mimic substrate conversion by enzymes. In this chapter, the following examples will be presented: (1) an esterolytic MIP for phenolic esters that is prepared u

Blueberry fruit fly, Rhagoletis mendax Curran (Diptera: Tephritidae), females deposit a host-marking pheromone (HMP) on the surface of fruit immediately after oviposition that deters oviposition by female conspecifics. We collected this HMP from artificial oviposition devices and faeces. In subsequent two-choice bioassays, gravid females showed a strong preference for untreated oviposition devices

The effect molecular crowding, defined as the volume exclusion exerted by one soluble inert molecule upon another soluble molecule, has on the structure and self-interaction of lipid-free apoA-I were explored. The influence of molecular crowding on lipid-free apoA-I oligomerization and internal dynamics has been analyzed using electron paramagnetic resonance (EPR) spectroscopy measurements of nitr

In this paper we analyse the interplay between services and manufacturing. This is done through identifying and quantifying a direct link between manufacturing exports and the sales by service providers. For identification of this transmittance mechanism, with the help of highly detailed geographic data, we create a Localised Export Exposure (LEE) variable that captures the variation in demand for

After having emerged as primary contenders in the race for highly efficient optoelectronics materials, organolead halide perovskites (OHLP) are now being investigated in the nanoscale regime as promising building blocks with unique properties. For example, unlike their bulk counterpart, quantum dots of OHLP are brightly luminescent, owing to large exciton binding energies that cannot be rationaliz

The article is a result of field studies on the transformations of multicultural heritage in the east-central European cities of Cracow, Wrocław, Lviv and Chernivtsi. In all these cities, a majority of the pre-war populations (and in Wrocław, practically all pre-war residents) disappeared as a result of WWII. After decades of silence imposed by the ruling communist elites, collective memories of t

Revised recommendations for the names and symbols of the transfermium elements(atomic numbers 101-109) are presented along with the reasons for proposing them. The recommendations (ref. 1) of the Commission on Nomenclature of Inorganic Chemistry (CNIC) on the nomenclature of the transfermium elements (101-109, inclusive) were considered by the IUPAC Bureau at Guildford (UK) in September 1995. As a result of the various criticisms of the recommendations and theway that they had been processed, the Bureau decided to adopt the recommendations as provisional

Carbonyl exchange between [MX3(13CO)]–(MX = PdCl, PtCl or PtBr) and 12CO has been studied by IR and 13C NMR spectroscopies as a function of temperature. The exchange is first-order in both CO and complex concentration, and the activation entropies are negative, suggesting an associative reaction mechanism. There is a small solvent effect, but no chloride dependence. Activation parameters and rate Carbonyl exchange between [MX(3)((CO)-C-13)](-) (MX = PdCl, PtCl or PtBr) and (CO)-C-12 has been studied by IR and C-13 NMR spectroscopies as a function of temperature. The exchange is first-order in both CO and complex concentration, and the activation entropies are negative, suggesting an associative reaction mechanism. There is a small solvent effect. but no chloride dependence. Activation para