Amplification of CCND1 (cyclin D1 gene) in squamous cell carcinoma of the head and neck (SCCHN) is correlated to poor prognosis. The purpose of this study was to investigate whether CCND1 amplification is related to different subsites and also to DNA ploidy status and S-phase fraction (SPF). Biopsies from 67 patients with SCCHN were analysed for CCND1 amplification by fluorescence in situ hybridis

An experimental procedure to determine crack propagation rates under thermo-mechanical loading is presented. The experiments were conducted in-situ within an environmental scanning electron microscope, and images of the crack tip region were taken throughout the load cycles. The mechanical load was provided by a load controlled electrically driven load stage. Thermal heating of the crack region wa

The use of antibiotic prophylaxis is an accepted and widely practiced feature of modern surgery. The prevention and control of infection is a priority in healthcare worldwide, and the emergence of antibiotic resistance is a global phenomenon. Hence, rational use of antibiotics is essential. We discuss the guidelines published with regard to endourologic procedures and review the limited evidence c

A prospective study of normoalbutninuric diabetic patients was performed between 1997 and 2002 on 4097 type 1 and 6513 type 2 diabetic patients from the Swedish National Diabetes Register (NDR); mean study period, 4.6 years. The strongest independent baseline risk factors for the development of microalbuminuria (20-200 mug/min) were elevated HbA(1c) and diabetes duration in both types 1 and 2 diab

Four different dispersing agents for solubilisation of single walled carbon nanotubes (SWNT) are here compared. Dispersing agents used here can be divided into the group of organic solvents (dimethylformamide and cyclohexanone) and charged polymers (Nafion and chitosan). Charged polymers are more efficient dispersing agents allowing quicker dispersion of nanotubes compared to solvents. Moreover ch

Exopolyphosphatase/guanosine pentaphosphate phosphohydrolase (PPX/GPPA) enzymes play central roles in the bacterial stringent response induced by starvation. The high-resolution crystal structure of the putative Aquifex aeolicus PPX/GPPA phosphatase from the actin-like ATPase domain superfamily has been determined, providing the first insights to features of the common catalytic core of the PPX/GP

We report a flow-injection biosensor system with a capacitive transducer for assay and quality control of human immunoglobulin G (hIgG). The sensing platform is based on self-assembled monolayers (SAMs) of carboxylic acid terminated alkyl-thiols with covalently attached concanavalin A. The electrochemical characteristics of the sensor surface were assessed by cyclic voltammetry using a permeable r

OBJECTIVES: Neutrophils are activated and infiltrate the myocardium after ischemia and reperfusion. The involvement of neutrophils in irreversible reperfusion injury is suggested by numerous experimental studies. The aim of this study was to investigate markers of neutrophil activation following reperfusion of acute myocardial infarction (AMI) accomplished with percutaneous coronary intervention (

An atomic protein model with a minimalistic potential is developed and then tested on an α-helix and a β-hairpin, using exactly the same parameters for both peptides. We find that melting curves for these sequences to a good approximation can be described by a simple two-state model, with parameters that are in reasonable quantitative agreement with experimental data. Despite the apparent two-stat

We studied a ca 200 ha large forest site that holds an exceptionally large number of red-listed saproxylic beetles. We conducted pollen, charcoal and dendroecological analyses to reveal the long-term stand-level history of the site. We also investigated which of the red-listed saproxylic beetles recorded at the site are probably dependent on forest fires or structures that are created by fires. Po

The accurate quantitative analysis of high resolution ultraviolet spectra of ultra-sharp-lined early-type, chemically peculiar stars, obtained with the Goddard High Resolution spectrograph on the Hubble Space Telescope, has been made possible by the synergism between state-of-the-art astro-physics and state-of-the-art atomic spectroscopy. We illustrate this with several examples, including the dis

The structures of films of SP-B and mixtures of SP-B and DPPC at the air/water interface have been studied by neutron reflectometry (NR) and Langmuir film balance methods. From the film balance studies we observe that at very high pressures the isotherms of pure DPPC and SP-B/DPPC mixtures very nearly overlay one another, suggesting that the SP-B is being excluded from the film. The use of multipl

A total of 2261 (808 male, 1453 female) consecutive patients attending contact dermatitis clinics were patch tested to isoeugenol and its derivatives listed in the EU Inventory of Fragrance Ingredients. Positive reactions were found to isoeugenol in 40, transisoeugenol in 40, isoeugenyl acetate in 19, isoeugenyl benzoate in 4, isoeugenyl phenylacetate in 16, isoeugenyl methyl ether in 6 and benzyl

Aim: The aim of this study was to study the type and frequency of complications and change in weight after a laparoscopic gastrostomy procedure in 31 children with congenital heart disease, comparing patient groups of children with univentricular and biventricular circulation, and with completed and uncompleted cardiac surgery. Methods: The method used was that of a retrospective study of all 31 c

Collagen-binding protein (CNBP) synthesized by Aeromonas veronii is located conserved within the subcellular fraction. The results of this study show that 98% of the total CNBP produced by Aer. veronii is present in the extracellular medium, and that the remaining CNBP is distributed either on the cell surface, within the periplasm or anchored on the outer membrane. CNBP is specifically secreted f

Polyamines are organic cations, which are considered essential for normal cell cycle progression. This view is based on results from numerous studies using a variety of enzyme inhibitors or polyamine analogues interfering with either the metabolism or the physiological functions of the polyamines. However, the presence of non-specific effects may be hard to rule out in such studies. In the present