Here we investigated the impact of WNT5A signaling on aerobic glycolysis and evaluated its effects on breast cancer cell migration/invasion. WNT5A signaling reduced migration and lactate production and caused selective down-regulation of the glycolytic enzyme phosphofructokinase platelet-type (PFKP). These events occurred in parallel with a WNT5A-induced inhibition of β-catenin signaling. Support

Before replication becomes mainstream, the potential for generating theoretical knowledge better be clear. Replicating statistically significant nonrandom data shows that an original study made a discovery; replicating a specified theoretical effect shows that an original study corroborated a theory. Yet only in the latter case is replication a necessary, sound, and worthwhile strategy.

The transglucosylation reaction catalyzed by wild-type β-glucosidase CelB from hyperthermophilic Pyrococcus furiosus and active site mutants (M424K, F426Y, M424K/F426Y) was studied. The conversion of pentyl-β-glucoside to hexyl-β-glucoside in hexanol was used as a model transglucosylation reaction. Hydrolysis to glucose was a side reaction. The activity (rates of hydrolysis and transglucosylation)

The selectivity of preparations of α-chymotrypsin immobilized on Celite or polyamide and carrying out syntheses of di- and tripeptides in acetonitrile medium were studied. The study concerns the effect of mass- transfer limitations on three different kinds of selectivity: acyl donor, stereo- and nucleophile selectivities, defined respectively as the ratio of initial rates with different acyl donor

The hydrolysis and transphosphatidylation of lysophosphatidylcholine (LPC), with a partially purified preparation of phospholipase D (PL D) from Savoy cabbage, was investigated. These reactions were about 20 times slower than the hydrolysis of phosphatidylcholine (PC) in a micellar system. For the transfer reaction, 2 M glycerol was included in the media, which suppressed the hydrolytic reaction.

The stereoselective reduction of 2-pentanone by alcohol dehydrogenase from Thermoanaerobium brockii was studied at controlled water activity and at different reaction temperatures. The reaction rate increased when water activity was increased from 0.32 to 0.96 and when raising the temperature from 5°C to 40°C. The enantioselectivity, E, reached a plateau value at high water activities. The enantio

A soluble NAD-dependent hydrogenase contained in Alcaligenes eutrophus was evaluated as a coenzyme regenerating catalyst in an organic-aqueous two- phase (predominantly organic) system. The horse-liver alcohol-dehydrogenase (HLADH) catalyzed reduction of cyclohexanone to cyclohexanol was used as a model reaction. The impact of different solvents (selected to span a large variety of principal prope

The enantioselectivity (E) of lipases in esterifications of secondary alcohols with decanoic acid was studied in organic media. The enantioselectivity of 2-octanol differed greatly among the lipases used. Candida antarctica lipase was extremely selective (E= 9 000) while Candida rugosa lipase was much less selective (E= 1.7). Other enzymes (Lipozyme and lipases from Pseudomonas and Rhizopus arrhiz

Alcaligenes eutrophus cells containing a soluble NAD-dependent hydrogenase was evaluated as a NADH regenerating catalyst. In the presence of hydrogen and permeabilized A. eutrophus cells, NADH regeneration was achieved, and reductions catalyzed by horse liver alcohol dehydrogenase and lactate dehydrogenase proceeded to 90-99% conversion. The operational stability of the hydrogenase was improved by

Different electron mediators were evaluated for the electromicrobial reduction of chloropyruvate to chlorolactate. The reaction was catalyzed by a mediatol-dependent D-lactate dehydrogenase in whole cells of Proteus vulgaris and yielded D-(S)-chlorolactate with high optical purity (> 97% e.e.). The ideal mediatol should favor a high rate in the enzymatic reaction and a low rate in the nonenzymatic

This paper presents a previously unreported method of laryngeal vocal sound production that is capable of producing pitches even higher than the whistle register (M3). Colloquially known as the glottal whistle (here referred to as M4), this method has a wider range than M3 and features frequent instances of biphonation, which is of interest for those involved with contemporary and improvised music

In this talk the FKS subtraction method for next-to-leading order (in QCD) computations is reviewed and its automation in the computer code MadFKS is presented. As a proof of concept, some results in the form of plots for distributions at next-to-leading order in e+e− → 2, 3 and 4 jets are shown by interfacing this code to BlackHat and Rocket.

We present a next-to-leading order calculation of Higgs boson production plus one and two jets via gluon fusion interfaced to shower Monte Carlo programs, implemented according to the POWHEG method. For this implementation we have used a new interface of the POWHEG BOX with MadGraph4, that generates the codes for generic Born and real processes automatically. The virtual corrections have been take

We present the complete next-to-leading-order (NLO) contributions to the pp→e+νeμ-ν̄μbb̄+X process in the four-flavor scheme, i.e., with massive b quarks, and its contribution to the H→WW(*) →llνν measurement in the 1-jet bin at the LHC. This background process includes top pair, single top, and non-top-quark resonant contributions. The uncertainty at NLO from the renormalization and factorization