Lactate dehydrogenase has been purified by precipitation with a bis-ligand. The precipitating agent in this case was Bis-NAD. This approach of affinity precipitation is also applicable to other enzymes.

The reactive triazine dye, Cibacron Blue F3G-A, has been covalently attached to microparticulate porous silica and used for the resolution of a number of complementary proteins by high-performance liquid affinity chromatography (HPLAC). Cibacron Blue F3G-A was converted to its 6-aminohexyl derivative by reaction with 1,6-diaminohexane and coupled to γ-glycidoxypropyltrimethoxysilane-activated poro

Three methods were developed for preparing alginate gels containing cells that are stable in phosphate containing media. In Method I preformed alginate beads containing entrapped cells were treated with polyethyl eneimine followed by glutaraldehyde. In Method II alginate sol was treated with a carbodiimide and N-hydroxysuccinimide (to form active esters), mixed with cells and extruded into calcium

I denna master class går forskarna igenom huvudpoängerna i den pupulärvetenskapliga rapporten Framtidens möten.Projektet handlar om nya modeller för affärer, interaktion och kommunikation inom mötesindustrin. Forskarna har undersökt hur pandemin påverkat mötesindustrin och om krisen fört med sig nya möjligheter. De har också studerat hur gäster i fysiska, hybrida och digitala möten och konferenser

Spores of Curvularia lunata were immobilized in polyacrylamide granules and in calcium alginate beads (2–3 mm in diam.). Germination of the spores, initiated by the addition of nutrients, resulted in an even distribution of mycelium throughout the beads after 48 h. Such beads were used for the conversion of cortexolone to cortisol by steroid-11β-hydroxylation. In order to improve the steroid trans

An enzyme-coenzyme complex was immobilized on the surface of a glassy carbon electrode and investigated with cyclic voltammetry in ethanol-containing buffers. The complex consists of an Liver Alcohol Dehydrogenase molecule to which an NAD-analogue is covalently attached via its straight six-carbon, spacer. One cycle was observed but repeated recycling could not be carried out. presumably due to ca

Whole cells of Arthrobacter simplex were immobilized in a living state in calcium alginate gel. The bacteria showed steroid-Δ1-dehydrogenase activity and the production of prednisolone from cortisol was investigated. The Δ1-dehydrogenase activity of the immobilized cells could be increased about ten-fold by incubation in nutrient media (e.g., containing 0.5% peptone abd 0.2% glucose). The reason f

The present facsimile of The Rudiments or Principles of the German flute (1729)—now republished for the first time since 1729—is a separate supplement to the comparativestudy Les Traditions en Mouvement Principes de la Flute Traversiere ou Flute d’Allemagne (1707) by Jacques Hotteterre Le Romain and The Rudiments or Principles of the German flute (1729) the first English translation of Hotteterre’

Saccharomyces cerevisiae was immobilized in calcium alginate gel together with varying concentrations of iron oxide, in the form of magnetite or a colloidal ferrite suspension, Ferrofluid™. Inclusion of magnetic material apparently had no adverse effect on the yeast cells as judged from their fermentation capacity, their operational stability as well as their ability to propagatein situ in the pre

This paper describes the preparation of a bifunctional NAD compound (Bis-NAD) and its properties as a complexing/precipitating agent for the tetrameric enzyme lactate dehydrogenase (LDH).

To plan, design and build with diversity in mind is a complex process. While goals such as inclusion, participation and social sustainability may be present in the vision for a future product, service or environment, studies show that the initial vision isn’t always realized in the end result. There are still far too many products, services and environments that are hard to access or use for parts

Introduction. The ability to read and interpret different types of text can be significantly impacted by neuropsychiatric disabilities, which affect cognitive abilities. This paper explores and reviews previous studies within library and information science on neuropsychiatric disabilities in relation to reading practices. Method. Searches were performed in LISA and LISTA to identify research on rIntroduction. The ability to read and interpret different types of text can be significantly impacted by neuropsychiatric disabilities, which affect cognitive abilities. This paper explores and reviews previous studies within library and information science on neuropsychiatric disabilities in relation to reading practices. Method. Searches were performed in LISA and LISTA to identify research on r

The present study highlights two important parameters of flute playing and musicalexpression: Sound and Articulation

A preparation of living Arthrobacter simplex cells immobilized in polyacrylamide gel, which showed steroid-Δ1-dehydrogenase activity, was studied. The entrapped microorganisms catalyzed the transformation of cortisol to prednisolone and this reaction was followed spectrophotometrically or with the aid of thin layer chromatography (TLC) and high pressure liquid chromatography (HPLC). About 40% of t