The Synthesis of Three AMP-Analogues: N6-(6-Aminohexyl)-adenosine 5′-Monophosphate, N6-(6-Aminohexyl)-adenosine 2′,5′-Bisphosphate, and N6-(6-Aminohexyl)-adenosine 3′,5′-Bisphosphate and Their Application as General Ligands in Biospecific Affinity Chromatography
Phosphorylation of 6‐chloropurine riboside with phosphorus oxychloride and phosphorus trichloride gave a mixture of the two isomers, 6‐chloropurine‐riboside 2′,5′‐bisphosphate and 6‐chloropurine‐riboside 3′,5′‐bisphosphate. Reaction with 1,6‐diaminohexane followed by resolution of the isomeric mixture on a Dowex 1‐X2 column yielded N6‐(6‐aminohexyl)‐adenosine 2′,5′‐bisphosphate and N6‐(6‐amin