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Regulation of callose synthase activity in situ in alamethicin-permeabilized Arabidopsis and tobacco suspension cells

Background: The cell wall component callose is mainly synthesized at certain developmental stages and after wounding or pathogen attack. Callose synthases are membrane-bound enzymes that have been relatively well characterized in vitro using isolated membrane fractions or purified enzyme. However, little is known about their functional properties in situ, under conditions when the cell wall is int

Searching for Celiac Disease Screening-detected celiac disease in an HLA-genotyped birth cohort

Popular Abstract in Swedish Celiaki, även kallat glutenintolerans, är en tarminflammation orsakad av vissa sädesslag i kosten och förekommer hos både barn och vuxna. Sjukdomen drabbar ca 1% av befolkningen i västvärlden och betraktas som en av de vanligaste kroniska sjukdomarna bland svenska barn. Många individer med sjukdomen är oupptäckta och kan bara hittas via s.k. screening, d.v.s. att man leObjectives: Celiac disease is a common immune mediated enteropathy strongly associated with HLA-DQB1*02 (DQ2), *0302 (DQ8), or both and the presence of tissue transglutaminase autoantibodies (tTGA). Prevalence studies have revealed that most affected individuals go undetected because of subclinical signs or being asymptomatic rendering screening a method for identification. However, less is known

Field-Dependent DNA Mobility in 20 nm High Nanoslits.

The transport behavior of lambda-DNA (48 kbp) in fused silica nanoslits is investigated upon application of electrical fields of different strengths. The slit dimensions are 20 nm in height, 3 microm in width, and 500 microm in length. With fields of 30 kV/m or below, the molecules move fluently through the slits, while at higher electrical fields, the DNA molecules move intermittently, resulting

Soil erosion estimation based on rainfall disaggregation

Abstract in UndeterminedSoil loss estimation remains one of the most difficult research tasks all over the world. Current simulation tools are still not detailed enough to allow for realistic scenarios to handle soil erosion problems. A common problem is the lack of rainfall data at a sufficient level of detail. The present study uses a cascade disaggregation model to generate short time scale rai

Moderate hyperkalemia in hospitalized patients with cirrhotic ascites indicates a poor prognosis

Objective. Development of ascites in patients with liver cirrhosis is an ominous sign with a poor outcome. A liver transplantation must be considered, and it then becomes important to know if there are any factors indicating a worsened prognosis. Material and methods. We used official registers for a follow-up study of at least 5 years considering the prognosis of 155 prospectively recruited in-pa

Measurements of cold nuclear matter effects in J/Psi in the PHENIX experiment via deutron-gold collisions

A new calculation of RdAu has been performed using the 2003 d+Au data and the higher-statistics 2005 p+p data. These nuclear modification factors are compared to calculations using nuclear-modified PDFs and a J/ψ breakup cross section is extracted. These values are then used to project the cold nuclear matter effects in Au+Au collisions. Additionally, a more data-driven projection is performed.

Downregulation of miR-92a Is Associated with Aggressive Breast Cancer Features and Increased Tumour Macrophage Infiltration.

BACKGROUND: MicroRNAs are small non-coding RNAs involved in the regulation of gene expression on a posttranscriptional level. These regulatory RNAs have been implicated in numerous cellular processes and are further deregulated in different cancer types, including breast cancer. MiR-92a is part of the miR-17∼92 cluster, which was first reported to be linked to tumourigenesis. However, little is kn

Evidence-based Or Traditional, Treatment of Cancer-related Lymphedema

The incidence of lymphedema (LE) related to treatment of women's cancer (breast and gynecologic) is as high as 40%. Treatment of LE varies around the world but was decades ago initially based on programs including manual lymph drainage (MLD), compression, skin care and easy exercise. With accumulating evidence and experience, it is time to consider if altering these treatment principles is needed.

Efficacy of the natural antioxidant astaxanthin in the treatment of functional dyspepsia in patients with or without Helicobacter pylori infection: A prospective, randomized, double blind, and placebo-controlled study

Objectives: The aim of this study was to evaluate the efficacy of the natural antioxidant astaxanthin in functional dyspepsia in different doses and compared with placebo. Design: The study was a controlled, prospective, randomized, and double blind trial. Participants: Patients with functional dyspepsia, divided into three groups with 44 individuals in each group (placebo, 16 mg, or 40 mg astaxan

Effect of Dynamic Channelling on the Transient-state Kinetics of Coupled Enzyme Reactions

Evidence is presented to show that the presence of a channelled reaction branch in a coupled two-enzyme reaction leads to a slower overall relaxation of the system towards steady state following perturbations of the reaction conditions. A predominant part of the total reaction flux change resulting from perturbations may be governed by transients that are more rapid in the presence than in the abs

Islet amyloid polypeptide-induced membrane leakage involves uptake of lipids by forming amyloid fibers

Fibril formation of islet amyloid polypeptide (IAPP) is associated with cell death of the insulin-producing pancreatic beta-cells in patients with Type 2 Diabetes Mellitus. A likely cause for the cytotoxicity of human TAPP is that it destroys the barrier properties of the cell membrane. Here, we show by fluorescence confocal microscopy on lipid vesicles that the process of hIAPP amyloid formation

Families' needs when a child is long-term ill: A literature review with reference to nursing research.

This article reports a literature review which draws together findings targeting families' needs when a child is long-term ill. The databases PubMed and CINHAL were searched from 1999 to 2003 during February 2004. The search terms were child health care, family caregivers, and needs and combinations of these. The search was limited to articles published in English and the Scandinavian languages. T

Dual-broadband rotational CARS thermometry in the product gas of hydrocarbon flames

Coherent anti-Stokes Raman spectroscopy (CARS) is an established technique for gas-phase thermometry in combustion. In this work, rotational CARS in the dual-broadband approach has been investigated for flame thermometry in the product gas of ethylene/air flames in a wide range of equivalence ratios (0.5 < phi < 2.5). The nitrogen lines dominated the rotational CARS spectra in the whole equivalenc