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Cortex synthesis during Bacillus subtilis sporulation depends on the transpeptidase activity of SpoVD.

The nonessential process of peptidoglycan synthesis during Bacillus subtilis sporulation is one model to study bacterial cell wall biogenesis. SpoVD is a class B high-molecular weight penicillin-binding protein that is specific for sporulation. Strains lacking this protein produce spores without the peptidoglycan cortex layer and are heat-sensitive. The detailed functions of the four different pro

Probing the smallest soot particles in low-sooting premixed flames using laser-induced incandescence

In this work we investigate nascent soot particles by analyzing laser-induced incandescence (LII) signals obtained in low-sooting premixed flames. The analysis covers two data sets obtained in separate experimental campaigns. The first data set was obtained in a previous work (Mouton et al., 2013) in methane/oxygen/nitrogen flames (equivalence ratio range 1.95 < Phi < 2.32) at 26.7 kPa, whereas th

Time trends between 1987 and 2007 for perfluoroalkyl acids in plasma from Swedish women.

Perfluoroalkyl acids (PFAAs) are a large group of chemicals which are highly persistent in both nature and humans. The use of the most prominent ones, perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA), was reduced in the early 21st century, and since then levels in human matrices have decreased. However, these two compounds have been exchanged by other PFAAs, for which time trends

Biochar increases arbuscular mycorrhizal plant growth enhancement and ameliorates salinity stress

We examined combined effects of biochar, arbuscular mycorrhizal (AM) fungi and salinity on plant growth and physiology to test whether and how biochar influences AM fungi mediated growth and nutrition enhancements, and whether and how biochar provides amelioration in salt stressed soils. We carried out a full three-factorial greenhouse experiment with Lactuca sativa; and a second study with a wide

Season of birth and risk of Hodgkin and non-Hodgkin lymphoma

Infectious etiologies have been hypothesized for Hodgkin and non-Hodgkin lymphoma (HL and NHL) in early life, but findings to date for specific lymphomas and periods of susceptibility are conflicting. We conducted the first national cohort study to examine whether season of birth, a proxy for infectious exposures in the first few months of life, is associated with HL or NHL in childhood through yo

Prolactin insufficiency but normal thyroid hormone levels after cranial radiotherapy in long-term survivors of childhood leukaemia.

BACKGROUND: Acute lymphoblastic leukaemia (ALL) patients treated with cranial radiotherapy (CRT) have an increased risk of GH deficiency (GHD). Little is known about insufficiencies of prolactin (PRL) and TSH, but also lactation failure has been reported in this population. OBJECTIVE: To study the long-term outcome of CRT on PRL and thyroid hormone levels in GHD ALL patients, and the prevalence of

High salt buffer improves integrity of RNA after fluorescence-activated cell sorting of intracellular labeled cells.

Over the past years, massive progress has been made in the ability to collect large-scale gene expression data from a limited sample size. Combined with improvements in multiplex flow cytometry-based techniques, this has made it possible to isolate and characterize specific cellular subtypes within heterogeneous populations, with a great impact on our understanding of different biological processe

National assessment of validity of coding of acute mastoiditis: a standardised reassessment of 1966 records

Objectives To investigate the internal validity of the diagnosis code used at discharge after treatment of acute mastoiditis. Design Retrospective national re-evaluation study of patient records 19932007 and make comparison with the original ICD codes. Setting All ENT departments at university hospitals and one large county hospital department in Sweden. Participants A total of 1966 records were r

Extracting accurate strain measurements in bone mechanics: A critical review of current methods

Osteoporosis related fractures are a social burden that advocates for more accurate fracture prediction methods. Mechanistic methods, e.g. finite element models, have been proposed as a tool to better predict bone mechanical behaviour and strength. However, there is little consensus about the optimal constitutive law to describe bone as a material. Extracting reliable and relevant strain data from

An estimate of post-depositional remanent magnetization lock-in depth in organic rich varved lake sediments

We studied the paleomagnetic properties of relatively organic rich, annually laminated (varved) sediments of Holocene age in Gyltigesjon, which is a lake in southern Sweden. An age-depth model was based on a regional lead pollution isochron and Bayesian modelling of radiocarbon ages of bulk sediments and terrestrial macrofossils, which included a radiocarbon wiggle-matched series of 873 varves tha

Does inbreeding affect gene expression in birds?

Inbreeding increases homozygosity, exposes genome-wide recessive deleterious alleles and often reduces fitness. The physiological and reproductive consequences of inbreeding may be manifested already during gene regulation, but the degree to which inbreeding influences gene expression is unknown in most organisms, including in birds. To evaluate the pattern of inbreeding-affected gene expression o

Long-term mortality rate in rheumatoid arthritis patients with disease onset in the 1980s.

Objective: To investigate the mortality rate and possible early predictive factors of mortality after 19-23 years in a cohort of patients with rheumatoid arthritis (RA) followed prospectively from disease onset. Patients and methods: A community-based cohort of 183 patients (63% female) with RA and disease duration < 2 years was recruited 1985-1989. The patients were followed yearly from diagnosis

Myeloid-related protein 14 promote inflammation and injury in meningitis.

Neutrophilic inflammation often persists over days despite efficient antibiotic treatment and contributes to brain damage in bacterial meningitis. We proposed here that MRP14, an abundant cytosolic protein in myeloid cells, acts as an endogenous danger signal, driving inflammation and aggravating tissue injury.

Postprandial Glycemia, Insulinemia, and Satiety Responses in Healthy Subjects after Whole Grain Rye Bread Made from Different Rye Varieties. 1

Rye products typically induce low insulin responses and appear to facilitate glucose regulation. The objective of this study was to investigate differences in postprandial glucose, insulin, and satiety responses between breads made from five rye varieties. Breads made from whole grain rye (Amilo, Rekrut, Dankowski Zlote, Nikita, and Haute Loire Pop) or a white wheat bread (WWB) were tested in a ra

Mostly empty words--what the discourse of "choice" in health care does.

PURPOSE: This paper has two purposes: one is to analyse how the policy of freedom of choice emerged and was formed in the Swedish health care discourse; the second is related to how free choice influences the discourse in health care and how subjects are formed within the field, i.e. what the language of choice in health care does. DESIGN/METHODOLOGY/APPROACH: The research strategy is inspired by

Sociodemographic and disease-related factors are associated with patient-reported anxiety and depression in spondyloarthritis patients in the Swedish SpAScania cohort.

Anxiety and depression are common among patients with rheumatic diseases. This study aims to explore which factors are associated with self-reported anxiety and depression in a well-defined cohort of spondyloarthritis (SpA) patients. In 2009, 3,711 patients from the SpAScania cohort were sent a postal questionnaire to assess health-related quality of life (HRQoL) and physical and mental functionin

Modified graphite filled natural rubber composites with good thermal conductivity

The rubber composites with good thermal conductivity contribute to heat dissipation of tires. Graphite filled natural rubber composites were developed in this study to provide good thermal conductivity. Graphite was coated with polyacrylate polymerized by monomers including methyl methacrylate, n-butyl acrylate and acrylic acid. The ratios between a filler and acrylate polymerization emulsion and