Heterologous Expression of Fungal Enzymes in two Aspergillus oryzae strains: A Comparative Study for Sustainable Plastic Recycling
This thesis explores the heterologous expression of six fungal enzymes with potential polyurethane (PUR) degrading activity in two strains of the filamentous fungus Aspergillus oryzae: the standard Ory6 and a knock-out variant with disrupted transcription factors to potentially reduce native protease activity. Each enzyme was expressed in three gene formats: a native version (including any introns
