An update and optimisation of oligonucleotide probes targeting methanogenic Archaea for use in fluorescence in situ hybridisation (FISH)
Fluorescence in situ hybridisation (FISH) is a common and popular method used to investigate microbial populations in natural and engineered environments. DNA oligonucleoticle probes require accurate determination of the optimal experimental conditions for their use in FISH Oligonucleotides targeting the rRNA of methanogenic Archaea at various taxonomic levels have previously been published, altho