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For the detection of Enterovirus, we devised a direct economical method of polymerase chain reaction which does not require a previous extraction of ribonucleic acid and uses infected cell culture supernatants. The system was developed on the basis of universal primers of Enterovirus and specific primers of vaccinal strain Sabin 1. The achieved results proved that the non-existence of methods of R
This paper reported the appraisal of a novel technology for the detection of enterovirus in sewage based on a viral recovery method using polyethylenglycol as a concentrating agent and on the combined use of viral isolation and polymerase chain reaction as viral detection and identification techniques. It was also confirmed that the viral recovery method is highly efficient since it allows to reco
During the epidemic of neuropathy occurred in Cuba from 1992 to 1994 viral agents antigenically related to the Coxsackie viruses were isolated from the cerebrospinal fluid of patients. To establish a function of these viruses in the etiopathogenesis of the disease, strains 47/93 IPK identified as Coxsackie A9 and the strain 44/93 of light cytopathic effect were selected to conduct a study of their
The polymerase chain reaction techniques was introduced for the intratypic characterization of Poliovirus. Primers were used only to promote the amplification of the Sabin vaccine strains proved by electrophoretic run of the amplified DNA products (Sabin 1-97 pb, Sabin 2-71 pb, Sabin 3-44 pb) and whose specificity was satisfactorily verified. 23 Cuban poliovirus strains isolated and identified at
The focusing and coherence properties of the NanoMAX Kirkpatrick–Baez mirror system at the fourth-generation MAX IV synchrotron in Lund have been characterized. The direct measurement of nano-focused X-ray beams is possible by scanning of an X-ray waveguide, serving basically as an ultra-thin slit. In quasi-coherent operation, beam sizes of down to 56 nm (FWHM, horizontal direction) can be achieve
The genomic characterization of the strain of light cytopathic effect by nucleotide sequence is presented. It was possible to sequence 90% of the strain of light cytopathic effect. A close relationship with Coxsackie A9 was observed. The greatest mutations occurred in the region of the genome that codifies for the structural proteins. Therefore, it may be considered as a variant of Coxsackie A9 ne
During the epidemic neuropathy occurred in Cuba from 1992 to 1993, viral isolations antigenically connected with Coxsackie viruses were obtained from the cerebrospinal fluid of patients. Virological, epidemiological, toxicologic, nutritional, immunological and histopathological investigations were made. Though the disease was related to toxic and nutritional factors, it has been impossible to iden
A series of experiments was made at the Virology Department of the "Pedro Kourí" Institute of Tropical Medicine aimed at obtaining new evidences on the possible antigenic relations existing between the viruses isolated from patients with epidemic neuropathy and the structures of the human nervous system. According to the results it may be finally inferred that the persistence and/or autoimmunity m
An increase of the cases of viral meningoencephalitis was reported in our country from October to December, 1995. 23 faeces specimens were received from 2 provinces aimed at obtaining the viral isolation by their inoculation in cell culture and in newborn mice at the same time in order to compare the sensitivity of both systems in isolation. A higher number of isolations was obtained in cell cultu
The polymerase chain reaction (PCR) was developed in order to identify the respiratory syncytial virus by using the reference strain. The high sensitivity and specificity obtained show the PCR utility for detecting the RSV genoma and its application on the diagnosis.
Twenty-one respiratory syncytial virus (RSV) strains isolated from one outbreak in Havana, Cuba (1994 to 1995), were analyzed to determine their relatedness. All isolated strains were classified as subgroup A by monoclonal antibodies. Of 21 RSV strains examined, 20 were classified as having restriction pattern NP4 and only 1 was classified as having restriction pattern NP5.
The aim of this study was to develop a polymerase chain reaction (PCR) for the detection of respiratory syncytial virus (RSV) genomes. The primers were designed from published sequences and selected from conserved regions of the genome encoding for the N protein of subgroups A and B of RSV. PCR was applied to 20 specimens from children admitted to the respiratory ward of "William Soler" Pediatric
The aim of this study was to bring to light the meanings attributed to four concepts denoting problematic drinking—“alcoholism,” “alcohol dependence,” “alcohol misuse,” and “risky drinking”—and to investigate whether individuals’ preferences for using these concepts vary depending on their demographic characteristics, their stakeholder group membership, and their own drinking habits. These questio
We present an existence and stability theory for gravity–capillary solitary waves on the top surface of and interface between two perfect fluids of different densities, the lower one being of infinite depth. Exploiting a classical variational principle, we prove the existence of a minimiser of the wave energy E subject to the constraint I= 2 μ, where I is the wave momentum and 0 < μ< μ, where μ is
This study conducts an investigation and feasibility study on different Reynolds numbers (6000–12000) and Rotation numbers (0.05–0.25) in a matrix cooling geometry. An intended geometry that can be used in gas turbine blades is provided based on flow and heat transfer performance given in these Reynolds and rotation number ranges for stationary and rotational state and then compared with experimen
In this study, molecular properties of wheat starch from three different types of breads were analyzed using asymmetric flow field-flow (AF4) connected to multi-angle light scattering (MALS) and differential refractive index (dRI) detectors. This analysis allowed the determination of molecular properties, i.e. molar mass (M), root-mean-square radius (rrms), apparent density (ρapp) and conformation
The Port of Rotterdam is an important industrial cluster, comprising mainly oil refining, chemical production and power generation. In 2016, the port's industry accounted for 19% of the Netherlands’ total CO2 emissions. The Port of Rotterdam Authority is aware that the cluster is heavily exposed to future decarbonisation policies, as most of its activities focus on trading, handling, converting an
