Cyclin-dependent kinases (CDKs) that control cell cycle progression are regulated in many ways, including activating phosphorylation of a conserved threonine residue. This essential phosphorylation is carried out by the CDK-activating kinase (CAK). Here we examine the effects of replacing this threonine residue in human CDK2 by serine. We found that cyclin A bound equally well to wild-type CDK2 (C

Eukaryotic cell cycle progression is controlled by a family of protein kinases known as cyclin-dependent kinases (Cdks). Two steps are essential for Cdk activation: binding of a cyclin and phosphorylation on a conserved threonine residue by the Cdk-activating kinase (CAK). We have studied the interplay between these regulatory mechanisms during the activation of the major Saccharomyces cerevisiae

Cell cycle progression is regulated by cyclin-dependent kinases (cdks). The activity of cdks is tightly controlled by several mechanisms, including binding of subunits to cdks (cyclins and inhibitors), and phosphorylation events. This review focuses on the activating phosphorylation of cdks by an enzyme termed cdk-activating kinase (CAK). Two classes of CAKs have been identified: monomeric Cak1p f

Activating phosphorylation of cyclin-dependent protein kinases (CDKs) is necessary for their kinase activity and cell cycle progression. This phosphorylation is carried out by the Cdk-activating kinase (CAK); in contrast, little is known about the corresponding protein phosphatase. We show that type 2C protein phosphatases (PP2Cs) are responsible for this dephosphorylation of Cdc28p, the major bud

Eukaryotic cell cycles are controlled by the activities of cyclin-dependent kinases (cdks). The major cdk in budding yeast, Saccharomyces cerevisiae, is Cdc28p. Activation of Cdc28p requires phosphorylation on threonine 169 and binding to a cyclin. Thr-169 is phosphorylated by the cdk-activating kinase (CAK), Cak1p, which was recently identified as the physiological CAK in budding yeast. Here we p

Mitochondrial creatine kinase (Mi-CK) consists of octameric and dimeric molecules that are interconvertible. In the present study, the kinetic properties of purified chicken heart Mi-CK (Mi(b)-CK) dimers and octamers were investigated separately under highly controlled conditions. Gel-permeation chromatography was performed before and after kinetic measurements in order to clearly define the propo

The effect that extreme natural events have on biological diversity is relatively poorly known. We used a before–after control-impact (BACI) design to analyze changes in bird abundances and communities following Hurricane Gudrun, which struck southern Sweden in January 2005, felling 75 million m3 of forest and causing damage to 5% of forested areas (half a million hectares) in a few hours. We used

Monitoring soil moisture at the Earth'surface is of great importance for drought early warnings. Spaceborne remote sensing is a keystone in monitoring at continental scale, as satellites can make observations of locations which are scarcely monitored by ground-based techniques. In recent years, several soil moisture products for continental scale monitoring became available from the main space age

OBJECTIVE: To describe midwives' experiences of providing contraception counselling to immigrant women.METHODS: The study was conducted with a qualitative design, based on interviews followed by inductive content analysis. Ten midwives were interviewed, working at midwife-led prenatal clinics in immigrant-dense areas in southern Sweden.RESULTS: Midwives require knowledge and understanding of cultu

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Rapporten är baserad på den utredning som genomförts för myndighets räkning och publiceras f n enbart internt på myndigheten.

After an injury, an immediate reparative response is triggered to replace the damaged tissue, however, in fibrosis, cells remain active despite wound resolution causing a steady build-up of fibrotic tissue. In pulmonary fibrosis,there is a constant regeneration and remodelling of lung tissue where the thin architecture of alveoli become thickened, hampering efficient diffusion of oxygen, as seen i

The latest Gaia data release enables us to accurately identify stars that are more luminous than would be expected on the basis of their spectral type and distance. During an investigation of the 329 best solar twin candidates uncovered among the spectra acquired by the GALAH survey, we identified 64 such overluminous stars. In order to investigate their exact composition, we developed a data-driv

Purpose: Differences exist concerning when and how to perform lymph node dissection (LND) during radical prostatectomy due to lack of high-grade evidence to its safety and efficacy. We aimed to compare readmission rates between limited and extended LND during open radical prostatectomy (ORP) and robot-assisted radical prostatectomy (RARP). Materials and methods: We conducted a prospective trial of

Nexilin, encoded by the NEXN gene, is expressed in striated muscle and localizes to Z-discs, influencing mechanical stability. We examined Nexilin/NEXN in smooth muscle cells (SMCs), and addressed if Nexilin localizes to dense bodies and dense bands and whether it is regulated by actin-controlled coactivators from the MRTF (MYOCD, MKL1, MKL2) and YAP/TAZ (YAP1 and WWTR1) families. NEXN expression

Up until the mid 1960s you could still find significant remains of a medieval brick house in Halmstad. Since the building was rediscovered in the 1920s, numerous speculations about the house and its owners have been presented. It has been linked to the Kalente guild as well as to the guild of St. Gertrud. These hypotheses rest, however, on very flimsy basis. Thus, this article examines a whole newUp until the mid 1960s you could still findsignificant remains of a medieval brick housein Halmstad. Since the building was rediscoveredin the 1920s, numerous speculationsabout the house and its owners have beenpresented. It has been linked to the Kalenteguild as well as to the guild of St. Gertrud.These hypotheses rest, however, on veryflimsy basis. Thus, this article examines awhole new hypothes

På senare tid har allt fler aktörer engagerat sig för att ”hjälpa bina” och motverka bidöd. Men när köpcentrum, snabbmatskedjor och fastighetsbolag satsar på bikupor för honungsbin i stället för att hjälpa vilda bin riskerar man att göra mer skada än nytta, skriver fyra Lundaforskare, som uppmanar företagen att hjälpa till på rätt sätt.

Since the end of the Cold War, migration law and policy of the global North has been characterised by externalisation, privatisation and securitisation. These developments have been conceptualised as denying access to migrants and as politics of non-entrée. This article proposes to broaden the analysis, and to analyse unwanted migration as merely one form of international human mobility by relying