Introduction of the xylose pathway from Pichia stipitis into Saccharomyces cerevisiae enables xylose utilization in recombinant S. cerevisiae. However, xylitol is a major by-product. An endogenous aldo-keto reductase, encoded by the GRE3 gene, was expressed at different levels in recombinant S. cerevisiae strains to investigate its effect on xylose utilization. In a recombinant S. cerevisiae strai

The relation between the isoform distribution of the myosin 17 kDa essential light chain (LC17) and the mechanical properties of smooth muscle was investigated. The relative content of the basic (LC17b) and acidic (LC17a) isoelectric variants of the 17 kDa myosin light chain was determined in different mammalian smooth muscle tissues. The relative content of LC17b varied between muscles: rabbit re

Visits to nest holes by birds other than their owners is a familiar phenomenon for students of breeding biology. In this study, we evaluate that behavior using a transponder reading system. Eighty-five males and females were Fitted with transponders at the end of the incubation period or just after hatching. Nest boxes were fitted with transponder readers from just after hatching until all nestlin

Popular Abstract in Swedish Akut bukspottkörtelinflammation (akut pankreatit - AP) är en tämligen frekvent förekommande åkomma med en årlig incidens på 300-350/100 000 invånare i Sverige. De underliggande mekanismerna för den lokalt initierande skadan i bukspottkörteln, spridning till ett systemiskt (i hela kroppen) inflammatoriskt svar och den eventuella utvecklingen av organdysfunktion (organsviAcute pancreatitis (AP) is a common disease where underlying mechanisms for the local initiating events in the pancreas, the systemic dissemination of the inflammatory response and remote organ dysfunction still are unclear. The overall aim of the thesis was to study mechanisms of the inflammatory response in AP with special emphasis on local, systemic, and distant organ injury directed at differe

Swallowing problems are common, with a prevalence of 5-30% in different populations. Clinical work-up must start with a careful evaluation of the symptomatology, which should then lead to appropriate investigations. A crucial point in the evaluation of these patients is the comparison between the patient's symptoms and the findings during the examination. Symptoms and findings often do not match,

The gene encoding an alkaline active cyclodextrin glycosyltransferase (CGTase) from the alkaliphilic B. agaradhaerens LS-3C was cloned and sequenced. It encodes a mature polypeptide of 679 amino acids with a molecular mass of 76 488 Da. The deduced amino acid sequence of the mature CGTase revealed 99 and 95% identity to the CGTase sequences from the other B. agaradhaerens strains, DSM 8721T and 99

Study of film historiography.

We report on low-temperature transport measurements on single and double quantum dots defined using local gates to electrostatically deplete InAs nanowires grown by chemical beam epitaxy. This technique allows us to define multiple quantum dots along a semiconducting nanowire and tune the coupling between them.

Objective: We reviewed the literature on nodal staging in patients with bladder cancer treated with radical cystectomy and lymphadenectomy. Results: Fractionating the lymph node specimen significantly increases the node count, whereas results are contradictory as to whether that increase improves detection of positive nodes. Pathoanatomic data indicate that extending lymph node dissection to the a

The temporal contrast is classified into two main regimes, the nanosecond-scale and the picosecond-scale contrast prior to the main pulse. The Lund terawatt laser system is shown to be improved on the nano- and picosecond-scale by a factor of 10 and 50, respectively, when it was optimized for contrast but not for energy. Calculations are also presented to emphasize the role of angular dispersion o

A common problem during recovery of bioproducts by adsorption from particulate broths is fouling of the adsorbent material as a result of the interaction of cells and cell debris, which present negative charges, with the positively charged anion exchangers commonly used in bioprocesses. The effect of shielding an adsorbent with a layer of agarose on reducing the binding of cells while still allowi

The optical properties of pig heart tissue were measured after in vivo ablation therapy had been performed during open-heart surgery. In vitro samples of normal and ablated tissue were subjected to measurements with an optically integrating sphere set-up in the region 470-900nm. Three independent measurements were made: total transmittance, total reflectance and collimated transmittance, which mad

This paper is the third in a series which recounts experiences in a project conducted in a newly-created Department of the University of Portsmouth (see also Bednar et al, 2004 and Eglin et al, 2005). A variant of the Strategic Systemic Thinking (SST) Framework (Bednar, 2000) was used to support the 25 staff of the Department to explore possibilities for development in a range of areas of work rel

The electronic transitions of 16 neutral thermal double donors (TDD1-TDD16) and of nine positively charged species (TDD1-TDD9) were observed previously in infrared absorption spectra. An earlier species (TDD0) was detected, however, only in persistent photoconductivity and Hall effect studies. In the present work, lines related to the electronic transitions from the ground to excited states in bot

The Wilms tumor gene (WT1) encodes a zinc-finger containing transcription factor present in primitive hematopoietic progenitor cells. WT1 is also highly expressed in most cases of acute myeloid leukemia. Moreover, WT1 can interfere with induced differentiation of leukemic cell lines. These data suggest a function of WT1 in the maintenance of a primitive phenotype and a role in leukemogenesis by in

Optical Burst Switching (OBS) architectures without buffering capabilities are sensitive to burst losses. A proper routing strategy may help to reduce such congestion. In this letter we present a novel approach for OBS multi-path source routing problem. Our solution is based on non-linear optimization with a straightforward calculation of partial derivatives. Simulation results demonstrate that op