The Malmo protocol for immune tolerance induction uses intermittent injections of factor VIII/IX together with intravenous IgG and cyclophosphamide. In order to increase the production of antigen-antibody complexes, and also to improve cost-efficacy, we applied continuous infusion instead of intermittent injection in 5 high-responders (3 hemophilia A and 2 hemophilia B). All treatment attempts fai

Identifying genes involved in the disease pathways of complex diseases such as Rheumatoid arthritis (RA) has been shown to be very difficult. Despite decades of research still only a handful of genes have been convincingly identified as risk genes. RA is a common autoimmune disorder that affects approximately 1% of the population and knowledge of the contributing genetic factors would greatly incr

A purified 75-kDa myrosinase and a crude rapeseed myrosinase fraction were used as antigens to produce mouse anti-myrosinase monoclonal antibodies. The 75-kDa myrosinase was also used to produce a polyclonal rabbit antiserum. The antiserum and one monoclonal antibody reacted with three distinct rapeseed polypeptides of 75, 70 and 65 kDa (M75, M70 and M65, respectively). A second set of monoclonal

Bacillus subtilis ferrochelatase (EC 4.99.1.1), the final enzyme in protoheme IX biosynthesis, was produced with an inducible T7 RNA polymerase expression system in Escherichia coli and purified from the soluble cell fraction. It was crystallized from polyethylene glycol solution using the microseeding technique. The crystals diffract to a minimum Bragg spacing of 2.1 A. The space group is P4(2) w

In the present study, a new and versatile liquid-phase microextraction method is described. This method requires very simple and cheap apparatus and also a small amount of organic solvent. Eight microliters of 1-undecanol was delivered to the surface of solution containing analytes and solution was stirred for a desired time. Then sample vial was cooled by inserting it into an ice bath for 5 min.

This study evaluates factors involved at different stages in an IS outsourcing decision process. From a theoretical perspective, the motivation for IS outsourcing is often described as a result of three factors: cost reduction, access to technological expertise and focus on core competence. The aim of this paper is to evaluate motivational factors in an outsourcing decision process. The study uses

We study the pointwise behavior of Birkhoff sums S(n)phi(x) on subshifts of finite type for Holder continuous functions phi. In particular, we show that for a given equilibrium state mu associated to a Holder continuous potential, there are points x such that S(n)phi(x) - nE(mu)phi similar to an(beta) for any a > 0 and 0 < beta < 1. Actually the Hausdorff dimension of the set of such points is bou

We describe a very efficient method for fluorescent labeling of acidic structures in denervated skeletal muscle with rhodamine B. Rhodamine B at 50 ng/ml gave selective and distinct segmental labeling of denervated muscle fibers after 5-min incubation at room temperature. Labeling was also achieved at 4 degrees C. The labeling was disrupted by the ionophores monensin and nigericin, suggesting a la

Man kan identifiera fem stora kundsegment på energimarknaden, nämligen hushåll i småhuslägenheter, hushåll i flerbostadshusens lägenheter, fastighetsägare till flerbostadshus, unga hushåll samt medelålders och äldre hushåll. Jämfört med energiföretagens andra kundsegment inom exempelvis industri, handel, service eller myndigheter är hushållssegmenten oorganiserade. De innehåller många hushåll med

This thesis treats the problem of direct adaptive control of linear multivariable systems. The parametrization problem of adaptive control is discussed extensively. A pole-placement problem and a model-matching problem are formulated and interpreted in terms of model reference control. The problem is solved via a discussion on system invariants of multivariable systems as presented by Pernebo. The

The cells of most normal and malignantly growing tissues are connected by "adhering junctions", i.e. distinct sites of "homotypic" contact between the plasma membranes of two cells of the same or a similar kind, associated on the cytoplasmic side by a dense plaque at which often bundles of cytoskeletal filaments anchor. Of the various types of adhering junctions desmosomes are characteristic of ep

Sample preparation techniques based upon liquid membrane extraction offer a high degree of selectivity and enrichment power and use small amounts of organic solvents. They also provide convenient possibilities for direct and automated interfacing with chromatography systems and other analytical instruments.

This paper examines combinatorial problems in connection with single-peaked preference orderings on a unidimensional scale. A binary relation, dominance, is defined on the set of connected orderings. Relevant properties of the corresponding poset and cover graph are discussed. A formula for the number of pairs of connected orderings consistent with spatial single-peakedness is derived. The total n