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Enzyme flow immunoassay using a Protein G column for the screening of triazine herbicides in surface and waste water

A method for screening of triazine herbicides in surface and waste water is presented. The method is based on an enzyme flow immunoassay (EFIA) for the detection of the free fraction of a horse radish peroxidase (HRP)-labelled antigen (tracer). This was accomplished by trapping the bound tracer fraction in a Protein G column, allowing the residual free tracer fraction to pass and be detected spect

Calcium binding and disulfide bonds regulate the stability of Secretagogin towards thermal and urea denaturation

Secretagogin is a calcium-sensor protein with six EF-hands. It is widely expressed in neurons and neuro-endocrine cells of a broad range of vertebrates including mammals, fishes and amphibia. The protein plays a role in secretion and interacts with several vesicle-associated proteins. In this work, we have studied the contribution of calcium binding and disulfide-bond formation to the stability of

Children and Adolescents with Mobility Limitation

A group of children with special health care needs, those with mobility limitations, is the focus of this chapter. We discuss the impact of mobility limitation in childhood and adolescence among those who were either born with a mobility limiting condition or those who acquired a mobility limitation at a very young age. The purpose of this chapter is to provide an overview of common important conc

Genetic risk, adherence to a healthy lifestyle, and coronary disease

BACKGROUND Both genetic and lifestyle factors contribute to individual-level risk of coronary artery disease. The extent to which increased genetic risk can be offset by a healthy lifestyle is unknown. METHODS Using a polygenic score of DNA sequence polymorphisms, we quantified genetic risk for coronary artery disease in three prospective cohorts - 7814 participants in the Atherosclerosis Risk in

Impact on Diffusion Parameters Computation in Gas Diffusion Layers, Considering the Land/Channel Region, Using the Lattice Boltzmann Method

Diffusion phenomena through the gas diffusion layer (GDL) at the microscale are one of the most complex physical phenomena to be described in proton exchange fuel cell (PEFC) numerical models. Predicting transport parameter behavior in GDLs is a valuable stage to propose micro-architectural designs, which can improve the efficiency and performance of fuel cells (FCs). The purpose of this paper is

Medborgarskapande på olika villkor : Självbilder, skolkoder och syn på kunskap i den svenska gymnasieskolan

Citizens are not born, they are made. Based on fieldwork in two upper-secondary school classes in Sweden – an Introductory Programme class and a Social Science Programme class – this dissertation focuses on how pupils form their identities as citizens in a democratic society. Citizens are made in interaction with the society they inhabit, an interaction where power is always present. Although Swed

Process contributions of Australian ecosystems to interannual variations in the carbon cycle

New evidence is emerging that semi-arid ecosystems dominate interannual variability (IAV) of the global carbon cycle, largely via fluctuating water availability associated with El Niño/Southern Oscillation. Recent evidence from global terrestrial biosphere modelling and satellite-based inversion of atmospheric CO2 point to a large role of Australian ecosystems in global carbon cycle variability, i

T50 D.3 Monitoring protocol for lighting and daylighting retrofits

This document presents a monitoring protocol to assess the overall performance of a lighting and/or daylighting retrofit of a building. This protocol covers four key aspects:1. Energy use;2. Retrofit costs;3. Photometric assessment;4. User assessment.This document develops each aspect in detail, presenting the required measurements and necessary equipment as well as providing guidelines for data a

A silicon depleted North Atlantic since the Palaeogene : Evidence from sponge and radiolarian silicon isotopes

Despite being one of Earth's major geochemical cycles, the evolution of the silicon cycle has received little attention and changes in oceanic dissolved silica (DSi) concentration through geologic time remain poorly constrained. Silicon isotope ratios (expressed as δ30Si) in marine microfossils are becoming increasingly recognised for their ability to provide insight into silicon cycling. In parti

A new high-resolution Holocene tephra stratigraphy in eastern Iceland : Improving the Icelandic and North Atlantic tephrochronology

A new and improved Holocene tephra stratigraphy and tephrochronological framework for eastern and northern Iceland is presented. Investigations of a sediment sequence from Lake Lögurinn have revealed a comprehensive tephra record spanning the last 10.200 years. A total of 157 tephra layers have been identified, whereof 149 tephra layers have been correlated to its source volcanic system using geoc