Modification of bovine heart succinate dehydrogenase with ethoxyformic anhydride and Rose bengal: Evidence for essential histidyl residues protectable by substrates
Purified and membrane-bound succinate dehydrogenase (SDH) from bovine heart mitochondria was inhibited by the histidine-modifying reagents ethoxyformic anhydride (EFA) and Rose Bengal in the presence of light. Succinate and competitive inhibitors protected against inhibition, and decreased the number of histidyl residues modified by EFA. The essential residue modified by EFA was not the essential