Isolation of component C4 of human complement and its polypeptide chains
Component C4 of human complement was purified from fresh frozen plasma with a yield of 25% using an initial batch separation with quaternary diethyl-(2-hydroxypropyl)aminoethyl--Sephadex followed by column chromatography on DEAE-cellulose and gel filtration in Sephadex G-200. The final product was homogenous according to polyacrylamide gel electrophoresis and immunochemical methods. Low-speed sedi