We have studied the binding of Zn2+ to the hexa EF-hand protein, calbindin D(28k)-a strong Ca2+-binder involved in apoptosis regulation-which is highly expressed in brain tissue. By use of radioblots, isothermal titration calorimetry, and competition with a fluorescent Zn2+ chelator, we find that calbindin D(28k) binds Zn2+ to three rather strong sites with dissociation constants in the low microm

This paper presents a numerical investigation of the effect of different back sweep angles and exducer widths on the steady-state impeller outlet flow pattern of a centrifugal compressor with a vaneless diffuser. The investigations have been performed with commercial computational fluid dynamics (CFD) and in-house programmed one-dimensional (ID) codes. CFD calculations aim to investigate how flow

PURPOSE: To assess the risk of death in patients who survive more than 5 years after diagnosis of childhood cancer and to evaluate causes of death in fatal cases. PATIENTS AND METHODS: This was a population-based study in the five Nordic countries (Denmark, Finland, Iceland, Norway, and Sweden) using data of the nationwide cancer registries and the cause-of-death registries. The study cohort inclu

Identification of ectomycorrhizal (ECM) fungi is often achieved through comparisons of ribosomal DNA internal transcribed spacer (ITS) sequences with accessioned sequences deposited in public databases. A major problem encountered is that annotation of the sequences in these databases is not always complete or trustworthy. In order to overcome this deficiency, we report on UNITE, an open-access da

On 30 October 2003, an ongoing geomagnetic superstorm knocked down a part of the high- voltage power transmission system in southern Sweden. The blackout lasted for an hour and left about 50,000 customers without electricity. The incident was probably the most severe geomagnetically induced current ( GIC) failure observed since the well- known March 1989 Quebec blackout. The " three- phase'' storm

Three species of Candelariella in western North America, characterized by biatorine apothecia, are treated. Two new species are described, C. biatorina and C. lichenicola. Candelariella biatorina is a corticolous species growing in montane conifer forests in western North America. Candelariella lichenicola, described from northwestern Mexico, is a lichenicolous fungus that lacks an independent tha

Gliding birds continually change the shape and size of their wings(1-6), presumably to exploit the profound effect of wing morphology on aerodynamic performance(7-9). That birds should adjust wing sweep to suit glide speed has been predicted qualitatively by analytical glide models(2,10), which extrapolated the wing's performance envelope from aerodynamic theory. Here we describe the aerodynamic a

Potential impacts of projected climate change on biodiversity are often assessed using single-species bioclimatic 'envelope' models. Such models are a special case of species distribution models in which the current geographical distribution of species is related to climatic variables so to enable projections of distributions under future climate change scenarios. This work reviews a number of cri

The following names in Flora Nordica Vol. 6 are subject to nomenclatural action: Helianthemum oelandicum var. canescens (typified), Epilobium hornemannii (typified), Oenothera nuda (validated), Myriophyllum spicatum (emendation of typification), Viola rupestris subsp. relicta (typified), Hippophae rhamnoides (typified), Angelica archangelica subsp. littoralis (typified). - Flora Nordica Note no. 3

Two-dimensional temperature measurements using filtered Rayleigh scattering (FRS) have been demonstrated. A tunable single-longitudinalmode alexandrite laser was employed to provide the tunable narrow-linewidth ultraviolet laser beam at 254 nm. Isotopic-enhanced mercury was utilized as an ultraviolet atomic filter. The strong absorption of the filter enabled effective suppression of elastic backgr

Abstract in Undetermined A Comment on the Letter by Xia-Ji Liu et al., Phys. Rev. Lett. 87, 030404 (2001).

ims In atrial fibrillation (AF), a relation between electrocardiogram (ECG) fibrillatory wave amplitude and thrombus formation has been sought for long with conflicting results. In contrast, the possible relation between atrial fibrillatory rate obtained from the surface ECG and left atrial thrombus formation in patients with AF is unknown and was consequently evaluated in this study. Methods and

Bacteria use a number of mechanisms for coping with the toxic effects exerted by nitric oxide (NO) and its derivatives. Here we show that the flavohemoglobin encoded by the hmp gene has a vital role in an adaptive response to protect the soil bacterium Bacillus subtilis from nitrosative stress. We further show that nitrosative stress induced by the nitrosonium cation donor sodium nitroprusside (SN

Background: Most enveloped viruses bud from infected cells by a process in which viral intracellular core components interact with cytoplasmic domains of transmembrane spike glycoproteins. We have demonstrated previously that a tyrosine motif in the cytoplasmic domain of the Semliki Forest virus (SFV) spike glycoprotein E2 is absolutely essential for budding, In contrast, hardly anything is known

OBJECTIVES: 5-Hydroxytryptamine (5-HT) has a wide range of vascular effects mediated via specific receptors and it has been suggested to be a mediator in ischemic heart disease. The aim of the present study was to localise the 5-HT receptors within the vessel wall. METHODS: Epicardial coronary arteries obtained from patients undergoing cardiac transplantation, internal mammary arteries from heart

The conventional view that female reproductive success is unlikely to benefit from multiple mating has come under strong challenge in recent years. In the present study, we demonstrate that the time wild-caught reproductive female water pythons (Liasis fuscus) spent in the laboratory prior to oviposition affected both hatching success and the number of male microsatellite alleles detected in the b

In vivo magnetic resonance spectroscopy (MRS) addresses metabolic pathways and their steady states in different tissue types. The brain has by tradition, and due to technical limitations in other organs, been one of the tissues most studied by MRS, and both 1H- and 31P-MRS have been used. Although 31P-MRS is outstanding for the evaluation of sources of metabolic energy in the brain, 1H-MRS has bec