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Quantitative flow cytometry to understand population heterogeneity in response to changes in substrate availability in escherichia coli and saccharomyces cerevisiae chemostats

Microbial cells in bioprocesses are usually described with averaged parameters. But in fact, single cells within populations vary greatly in characteristics such as stress resistance, especially in response to carbon source gradients. Our aim was to introduce tools to quantify population heterogeneity in bioprocesses using a combination of reporter strains, flow cytometry, and easily comprehensibl

Impact of pH on the Treatment of Artificial Textile Wastewater Containing Azo Dyes Using Pond Systems

Two controlled experiments were operated to evaluate the impact of pH on the treatment efficiency of azo dyes within artificial textile wastewater using ponds as a polishing step. The objectives were to assess the (1) inflow water quality; (2) the presence of Lemna minor and the algae Oedogonium spp. on the dye removal and the outflow water quality; and (3) suitability of applying artificial texti

Lobaria pulmonaria in the southwestern Baltic area

Lungwort lichen, Lobaria pulmonaria (L.) Hoffm., was surveyed in the southwesternBaltic Area in 2016–2018. At each of the localities, the habitat ecology and lichen viability were investigated. A substantial decline was noted, particularly in Skåne. The commonest habitats for L. pulmonaria in southernmost Sweden are lime-maple and species-poor, oligotrophic beech forests, and the commonest substra

Auditor rotations and audit quality : A perspective from cumulative number of audit partner and audit firm rotations

PurposeThe purpose of this paper is to investigate the association between audit rotation – at the audit partner and audit firm level – and audit quality. As mentioned in the literature, audit rotation has several benefits, and one of them is it can bring a fresh look to audit tasks and subsequently improve audit quality. Moreover, audit itself can help a client to improve its financial reporting.

Geographies of the book (shop) : Reading women’s geographies in Enlightenment Edinburgh

This paper examines the place of women’s geographical reading in a centre of Enlightenment: Edinburgh, 1770–1810. It analyses two sets of booksellers’ records to identify key sites – women’s private libraries, Georgian domestic spaces, and women’s schools and boarding houses – in which women engaged with the geographical materials sold in the city’s bookshops: maps, globes, travel accounts, gazett

Return to work after stroke : A Swedish nationwide registry-based study

Objectives: A substantial proportion of individuals with stroke are of working age. After stroke, it is important to return to work (RTW), both for the individual's satisfaction with life and economically for society. The current comprehensive, long-term study aimed at investigating in what time period the RTW continues after stroke and what factors could predict RTW. Materials and methods: All in

Altiplanic aquifer exploration by multidisciplinary methods : Addressing the basis of sustainable use of groundwater

The sustainable use of groundwater relies on building up three stages: exploration, development and management. The exploration is crucial to understand the capabilities and limitations of aquifer units; e.g. volume of available water and potential yield, distinction of recharge and discharge zones, and the groundwater quality are developed in this stage. In the Altiplano, groundwater is probably

Liver metastases from colorectal cancer. Different strategies and outcomes

Patients with colorectal liver metastases (CRLM) increasingly undergo liver resections. The belief is that the resection or ablation of a tumor, when possible, is the only possibility of a cure. The classical strategy is where the primary colorectal tumor is resected as the first intervention, followed by resection of the liver metastasis at a second stage. The liver-first strategy is where preope

In-house validation of MPS-based methods in a forensic laboratory

Massively parallel sequencing (MPS) methods are increasingly applied in forensic casework. However, adequate validation guidelines are lacking. In this work, we describe our in-house validation of the ForenSeq DNA Signature Prep Kit (Verogen) for analysis of ancestry- and phenotype-informative SNPs. We also discuss in-house validation of MPS assays in general terms. When validating the SNP assay,

A multirate Neumann-Neumann waveform relaxation method for heterogeneous coupled heat equations

An important challenge when coupling two different time dependent problems is to increase parallelization in time. We suggest a multirate Neumann-Neumann waveform relaxation algorithm to solve two heterogeneous coupled heat equations. In order to fix the mismatch produced by the multirate feature at the space-time interface a linear interpolation is constructed. The heat equations are discretized

Expression of the RAI gene is conducive to apoptosis : studies of induction and interference

The RAI gene is also known as iASPP and PPP1R13L. Recent investigations have shown that the region encompassing RAI is important for the development of cancer in young and middle-aged persons. It has been speculated that the RAI product induces apoptosis by blocking NF-kappaB or inhibits apoptosis by blocking p53. Either way the gene could influence the survival of precancerous lesions. Here we re

Evaluation of sequential processing for the extraction of lipids, starch and proteins from wheat bran

In line with the need to better utilize agricultural resources, and valorize underutilized fractions, we have developed protocols to increase the use of wheat bran, to improve utilization of this resource to additional products. Here, we report sequential processing for extraction of starch, lipids, and proteins from wheat brans with two different particle sizes leaving a rest-material enriched in

Pneumococcus Infection of Primary Human Endothelial Cells in Constant Flow

Interaction of Streptococcus pneumoniae with the surface of endothelial cells is mediated in blood flow via mechanosensitive proteins such as the Von Willebrand Factor (VWF). This glycoprotein changes its molecular conformation in response to shear stress, thereby exposing binding sites for a broad spectrum of host-ligand interactions. In general, culturing of primary endothelial cells under a def

Persons' experiences of suffering from nephrotic syndrome.

BackgroundLittle is known about health and wellbeing among patients with nephrotic syndrome (NS), despite it being a serious condition in patients with renal failure. In order to promote health, it is important that both healthcare professionals and patients are aware of the signs and symptoms of the disease.ObjectivesThe aim was to explore patients’ experience of suffering from nephrotic syndrome