PIXE Analysis of Blood Serum Proteins, Separated by Gel Filtration
Gel filtration of blood serum was carried out with Sephadex G-200 gel and tris buffer at pH 7.8. The amount of protein in each of about 100 collected fractions was monitored using UV absorption. The fractions were concentrated and deposited onto Kimfol backing foils. From the PIXE results distributions of S, Fe, Cu and Zn were obtained in relation to the protein peaks, while elements like K, Ca an