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Two new vectors are described, the expression vector pB3 PGK and the CRE recombinase vector pCRE3. The pB3 PGK has a zeocin-selectable marker flanked by loxP sequences and an expression cassette consisting of the strong PGK1 promoter and the GCY1 terminator. The S. cerevisiae genes RKI1, RPE1, TAL1 and TKL1 were cloned in pB3 PGK and integrated in the locus of the respective gene, resulting in ove

Free volume and density gradient widths of the free surface of high molecular mass polystyrene was characterized by use of a pulsed low-energy positron lifetime beam. A density gradient in terms of mass density (g/cm(3)) versus depth (nm) was obtained from the experimental beam data using a novel approach, yielding a width of the density gradient of approximately 3.5 nm. The procedure accounted fo

In this paper we apply a microscopic nonequilibrium many body Keldysh Green's functions approach to an analysis of complex intersubband optical materials and devices. The calculated absorption/gain spectra are in very good agreement with transmission spectroscopy measurements of quantum cascade laser structures operating in the mid-infrared (midIR). The very good agreement reached between theoreti

The yrast superdeformed band in Zn-61 has been established using Si-28(Ar-36, 2pn)Zn-61 and Ca-40(Si-29, 2 alpha)Zn-61 fusion-evaporation reactions. The excitation energy of this band was determined via two transitions that link this band to the normally deformed states. Lifetime analysis of this band resulted in a quadrupole moment of Q(t) = 3.0 +/- (0.5)(0.4)e b, which corresponds to a deformati

The immature rat ovary contains VIP immunoreactive nerve fibres sparsely distributed around blood vessels, in the interstitial gland and around follicles. The VIP concentration, measured radioimmunologically, decreased significantly after PMSG treatment (10 i.u.), probably due to ovarian enlargement and oedema, while the total VIP content (total of 0.12 pmol in both ovaries) did not change after P

Ten years of full-scale experience with enhanced biological phosphorus removal (EBPR) has been evaluated. During the start-up period lack of carbon source was the main operational problem and a higher level of volatile fatty acids was secured by introducing a primary sludge hydrolysis. Acidic thermal sludge hydrolysis was used as the sludge treatment method at the plant during about three years. O

Fog deposition in December 1992 and February 1993 (two periods of several days in which fog occurred) was monitored at the location Speulderbos in The Netherlands. Fog droplet deposition was measured with eddy correlation and samples of fog water were taken with a string collector. At the same time, throughfall deposition was measured and throughfall water was sampled. The occult (fog/cloud) depos

A comparison, based on a study of how time is treated, between three development tools for real time expert system is exposed. After a presentation of the tools, some of their advantages and disadvantages arepinpointed. Since these tools are only a partial answer to the problem of real time within the expert system's area, a standpoint concerning a good use of each tool is given.

This paper addresses the question of why metal- deficient barium stars are not yellow symbiotic stars ( YSyS). Samples of ( suspected) metal-deficient barium ( mdBa) stars and YSyS have been collected from the literature, and their properties reviewed. It appears in particular that the barium nature of the suspected mdBa stars needs to be ascertained by detailed abundance analyses. Abundances are

This thesis consists of a collection of essays on coordination in games and competition in international oligopolies. The first essay presents a theoretical analysis of a simple coordination game in which players simultaneously choose efforts from a compact interval. The original game has a continuum of Pareto-ranked equilibria. In a noisy variant of the model an error term is added to each player

Popular Abstract in Swedish Avhandlingen behandlar reglering av Homogeneous Charge Compression Ignition (HCCI) motor dynamik. HCCI erbjuder möjligheten att kombinera hög effektivitet med väldigt låga emissioner. Återkopplad reglering är ett sätt att uppnå dessa mål. Avhandlingen diskuterar sensorer, återkopplingsvariabler och aktuatorer för sluten-loop reglering av HCCI förbränningen. Det är demoThe topic of the thesis is control of Homogeneous Charge Compression Ignition (HCCI) engine dynamics. HCCI offers a potential to combine high efficiency with very low emissions. In order to fulfill the potential benefits, closed-loop control is needed. The thesis discusses sensors, feedback signals and actuators for closed-loop control of the HCCI combustion. Closed-loop control of the HCCI combu

Protein kinase C (PKC) is a family of serine/threonine kinases which are subgrouped into classical (a, bI, bII, g), novel (d, e, h, q) and atypical (z, i/l) isoforms. It has been shown that PKCe induces neurite outgrowth in neuroblastoma cells and the effect is mediated via the regulatory domain. One aim of this study was to identify the structures in PKCe that mediate neurite outgrowth. This stu

A reaction-diffusion model was used to simulate a co-immobilized system utilizing the numerical method of orthogonal collocation. The production of ethanol from deproteinized whey using beta-galactosidase co-immobilized with Saccharomyces cerevisiae in calcium alginate gel beads was chosen as a model system. Calculated concentrations of lactose, glucose, galactose and ethanol were compared with ex

This paper deals with line-drawings, obtained from images of piecewise planar objects after edge detection. Such images are used, e.g., for navigation and recognition. In order to be a possible image of a three dimensional piecewise planar object, it has to obey some projective conditions. Criteria for a line-drawing to be correct is given in this paper, along with methods to find possible interpr

The aerobic degradation of phenanthrene by a Pseudomonas migulae strain under classical mechanical aeration and under photosynthetic oxygenation (using a Chlorella sorokiniana strain) in a two-phase partitioning bioreactor (TPPB) constructed with silicone oil as organic phase was investigated. When traditional mechanical aeration was used, an increase in the aeration and/or in the agitation rate e