Fractionation of the thylakoid membranes from tobacco. A tentative isolation of ‘end membrane’ and purified ‘stroma lamellae’ membranes
Thylakoids isolated from tobacco were fragmented by sonication and the vesicles so obtained were separated by partitioning in aqueous polymer two-phase systems. By this procedure, grana vesicles were separated from stroma exposed membrane vesicles. The latter vesicles could be further fractionated by countercurrent distribution, with dextran-polyethylene glycol phase systems, and divided into two
