Application of supercritical carbon dioxide(scCO(2))for the extraction of lipopolysaccharides (LPS) from Salmonella enterica subsp enterica PCM 2266
The paper describes a novel extraction procedure for lipopolysaccharides (LPS) from Salmonella enterica subsp. enterica (PCM 2266). Process parameters for the extraction of LPS from bacterial mass were optimized by carrying out a two-level fractional design experiment. Four parameters, namely temperature, CO2 flow rate, pressure and co-solvent composition were analyzed. The best crude extract yiel