An integrated process for purifying a 6.1 kilo base pair (kbp) plasmid from a clarified Escherichia coli cell lysate based on an ultra/diafiltration step combined with polymer/polymer aqueous two-phase system and a new type of chromatography is described. The process starts with a volume reduction (ultrafiltration) and buffer exchange (diafiltration) of the clarified lysate using a hollow fibre me

Chronic obstructive pulmonary disease (CCPD) is a disease primarily caused by cigarette smoking, which in turn has been shown to affect the susceptibility to and progression of airway infections. The question addressed in this study was how components from cigarette smoke could affect the defence mechanisms of T-cells and epithelial cells, and thereby contribute to the development of the COPD path

We have studied the dynamics of water molecules in six crystal structures of four human cytochromes P450, 2A6, 2C8, 2C9, and 3A4, with molecular dynamics simulations. In the crystal structures, only a few water molecules are seen and the reported sizes of the active-site cavity vary a lot. In the simulations, the cavities are completely filled with water molecules, although with similar to 20% low

We prove that steady periodic two-dimensional rotational gravity water waves with a monotone surface profile between troughs and crests have to be symmetric about the crest, irrespective of the vorticity distribution within the fluid.

I denna avhandling används samtalsanalys (”conversation analysis”) för att studera ”riskstrategier” i samtal mellan lärare, elever och föräldrar i grundskolan. Materialet består av 80 samtal som insamlats vid två olika tidpunkter: 45 st. inspelade 1992–93 (kvartssamtal, eller den mer formella benämningen enskilda samtal) och ytterligare 35 st. inspelade 2004 (utvecklingssamtal, den benämning som gIn this thesis, conversation analysis (CA) is applied to study “risk strategies” in parent-teacher-student conferences in the Swedish nine-year compulsory school. The material consists of 80 conferences collected at two different points in time: 45 from the period 1992–93 (at that time called kvartssamtal, lit. “quarter of an hour conference”) and another 35 collected in 2004 (at the present time,

Popular Abstract in Swedish Blodgruppsantigen finns på den röda blodkroppens cellyta och även på många andra celltyper. I denna avhandling har jag studerat gener och dess varianter (polymorfismer) som är ansvariga för uttrycket av tre blodgruppsantigener av kolhydratnatur, nämligen Pk, P och P1. Dessa utgör P- och GLOB-blodgruppssystemen och GLOB-kollektionen. Fem olika fenotyper förekommer då anThe clinically important carbohydrate P/GLOB blood group systems and collection give rise to both common (P1, P2) and rare (p, P1k, P2k) blood group phenotypes. The associated antibodies are implicated in severe transfusion reactions and recurrent spontaneous abortions. The aim of this study was to explore the molecular genetic basis of Pk, P and P1 antigen expression. Sequence analysis of the A4

The Materials Science Beamline ID11 at the European Synchrotron Radiation Facility in Grenoble, France is dedicated to research in materials science notably employing diffraction and scattering techniques. Either an in-vacuum undulator with a minimum gap of 5 mm or a 10 kW wiggler giving high-flux monochromatic X-rays generates the synchrotron radiation in the energy range 5–100 keV. The dominant

PURPOSE: To characterize the clinical phenotype, with emphasis on electrophysiology, of members of a Swedish family with autosomal dominant retinitis pigmentosa due to a novel mutation, F211L, in the peripherin/RDS gene. METHODS: Nine patients with autosomal dominant retinitis pigmentosa and two healthy family members underwent a full clinical evaluation including kinetic visual field testing, mea

The aims of this study were to describe which self-care, productivity, and leisure occupations individuals with multiple sclerosis (MS) perceive as difficult to perform on admission to rehabilitation and the individuals' own perception of the importance of, performance of, and satisfaction with these occupations. Whether the reported self-care, productivity, and leisure occupations were related to

Zeaxanthin, an important component in protection against overexcitation in higher plants, is formed from violaxanthin by the enzyme violaxanthin de-epoxidase. We have investigated factors that may control the maximal degree of conversion in the violaxanthin cycle. The conversion of violaxanthin to zeaxanthin in isolated spinach thylakoids was followed at different temperatures and in the presence

The present thesis is a study of Mediterranean trade 400-250 BC through an examination of transport amphorae from the ancient Cyrenaican city of Euesperides (Benghazi), Libya. The material comes from excavations conducted from 1999-2006 by the Society for Libyan Studies. Amphorae were used for the transportation of commodities such as wine and olive oil. Found in great numbers at the site they tes

A method was developed for isolating and sequencing proteins present in the extracellular matrix (ECM) of germlings and hyphae of filamentous fungi, Surface proteins of the cereal pathogen Bipolaris sorokiniana were labelled with a membrane impermeable biotinylating agent and extracted using a glycine-HCl buffer. Extracted proteins were purified by affinity binding to streptavidin-conjugated magne