Miniaturized (Ø 10 μm), multiplexed (>5-plex), and high-density (>100 000 spots cm(-2)) antibody arrays will play a key role in generating protein expression profiles in health and disease. However, producing such antibody arrays is challenging, and it is the type and range of available spotters which set the stage. This pilot study explored the use of a novel microspotting tool, Bioplume(TM)-cons

Little is known about the relationship between specific subtypes of treatment-associated motor complications and different domains of health-related Quality of Life (QoL) in patients with Parkinson's disease (PD). Larger studies that investigate these aspects within a cross-cultural setting are scarce.

Global leaders agree on the need to substantially decarbonize the global economy by 2050. This paper compares potential costs associated with different policy pathways to achieve tourism sector emission reduction ambitions (−50% by 2035) and transform the sector to be part of the mid-century decarbonized economy (−70% by 2050). Investment in emissions abatement within the tourism sector, combined

Concentration of nutrients and balance between nutrients in trees can affect tree vitality, and are dependent on soil conditions and atmospheric deposition. The aim of this investigation was to survey the concentration of nutrients in beech bark and to look for relationships with the frost sensitivity of the bark. Beech trees with bark lesions were compared to undamaged beech trees on five experim

Little is known about the contribution of bacteria and fungi to decomposition of different carbon compounds in arctic soils, which are an important carbon store and possibly vulnerable to climate warming. Soil samples from a subarctic tundra heath were incubated with C-13-labeled glucose, acetic acid, glycine, starch, and vanillin, and the incorporation of C-13 into different phospholipid fatty ac

Yields for J/psi production in Cu+Cu collisions at root s(NN) = 200 GeV have been measured over the rapidity range |y|< 2.2 and compared with results in p+p and Au+Au collisions at the same energy. The Cu+Cu data offer greatly improved precision over existing Au+Au data for J/psi production in collisions with small to intermediate numbers of participants, in the range where the quark-gluon plasma

We used a crossing experiment to investigate post-zygotic barriers that might limit introgression between a pair of closely-related, gynodioecious plant species - the widespread weed Silene vulgaris and the local Swedish endemic S. uniflora ssp. petraea. The study not only considered the effects of hybridization on conventionally-used (primary) fitness components such as seed set and progeny survi

Aberrant dopaminergic signaling is a critical determinant in multiple psychiatric disorders, and in many disease states, dopamine receptor number is altered. Here we identify a molecular mechanism that selectively targets D2 receptors for degradation after their activation by dopamine. The degradative fate of D2 receptors is determined by an interaction with G protein coupled receptor-associated s

1. Within a single year, long-distance migrants undergo a minimum of four cycles of fuel storage and depletion because their migrations have at least one stopover. Each cycle includes an almost twofold change in body mass (m(b)). Pervasive predation threats beg the question whether escape flight abilities keep up with such large changes in m(b). 2. We derive aerodynamic predictions how pectoral mu

Follicular lymphoma is the second most frequent type of non-Hodgkin's lymphoma in adults. The basic molecular defect consists of the t(14;18)(q32;q21) translocation, juxtaposing the B-cell lymphoma protein 2 gene BCL2 to the immunoglobulin heavy chain locus IGH@, and leading to the antiapoptotic BCL2 protein overproduction. Variations in the t(14;18) are rare and can be classified into two categor

Spherical brushes composed of diblock polyampholytes (diblock copolymers with oppositely charged blocks) grafted onto solid spherical particles in aqueous solution are investigated by using the primitive model solved with Monte Carlo simulations and by lattice mean-field theory. Polyampholyte chains of two compositions are considered: a copolymer with a long and a short block, A(100)B(10), and a c

We report on the performance of the third-order Douglas–Kroll ab initio model potential (DK3-AIMP) method-based electron-correlated spin–orbit calculations. Our treatment assumes that the problem can be separated into a spin–free correlation treatment and a spin–orbit calculation. The correlation effects were calculated using the multistate complete active space second-order perturbation method, a

Climate change and biological invasions are key processes affecting global biodiversity, yet their effects have usually been considered separately. Here, we emphasise that global warming has enabled alien species to expand into regions in which they previously could not survive and reproduce. Based on a review of climate-mediated biological invasions of plants, invertebrates, fishes and birds, we

Intracellular application of the sulfonylurea tolbutamide during whole-cell patch-clamp recordings stimulated exocytosis >5-fold when applied at a cytoplasmic Ca2+ concentration of 0.17 microM. This effect was not detectable in the complete absence of cytoplasmic Ca2+ and when exocytosis was elicited by guanosine 5'-O-(3-thiotriphosphate) (GTPgammaS). The stimulatory action could be antagonized by

We investigated whether cell proliferation and neurogenesis are altered in R6/2 transgenic Huntington's disease mice. Using bromodeoxyuridine (BrdU), we found a progressive decrease in the number of proliferating cells in the dentate gyrus of R6/2 mice. This reduction was detected in pre-symptomatic mice, and by 11.5 weeks, R6/2 mice had 66% fewer newly born cells in the hippocampus. The results w

Relapsing polychondritis is an autoinumme disease that affects cartilage in the ear, nose, and respiratory tract. A pathogenic immune response has been proposed and antibodies to several cartilage proteins are detected in sera from these patients. To investigate the role of the humoral immune response in relapsing polychondritis, we used the matrilin-1-induced relapsing polychondritis model. Mice

OBJECTIVE: To develop a new mouse model for arthritis using cartilage oligomeric matrix protein (COMP) and to study the role of major histocompatibility complex (MHC) and Ncf1 genes in COMP-induced arthritis (COMPIA). METHODS: Native (pentameric) and denatured (monomeric) COMP purified from a rat chondrosarcoma was injected into mice with Freund's adjuvant to induce arthritis. C3H.NB, C3H.Q, B10.P

Background. The aim was to investigate whether social network and social support factors can explain socioeconomic differences in the risk of consuming low amounts of vegetables, fruit and fruit juices. Method. The Malm Diet and Cancer Study was a prospective cohort study. The present cross-sectional study examined data from a subpopulation of 11,837 individuals that completed baseline examination