Bioelectrochemical Applications of Reactions Catalyzed by Immobilized Enzymes
The sensitivity of biosensors can be increased substantially by incorporation in a substrate recycling scheme. Based on the amplification of NAD+/NADH by the recycling reaction catalyzed by glycerol dehydrogenase (GDH) and diaphorase, NAD+/NADH can be determined amperometrically with around 1000 times higher sensitivity than for the substrate sensing mode. The sensitivity of enzyme electrodes were