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Screening and selection of Lactobacillus strains for use as adjunct cultures in production of semi-hard cheese

Thirty-three Lactobacillus strains were tested as adjuncts in a cheese model system. Eighteen strains originated from cheese (nine Lactobacillus spp. and nine Lb. paracasei/casei) and 15 from human intestinal mucosa (11 Lb. rhamnosus; three Lb. paracasei; one Lb. plantarum). Model cheeses weighing 120 g were made of cheese grains from full-scale production of washed curd semi-hard cheese (Herrgard

Experimental investigation on port-to-channel flow maldistribution in plate heat exchangers

Experiments have been conducted to analyze the flow and pressure distribution in a plate heat exchanger by measuring local port pressure distribution in a commercial plate heat exchanger. Flow rate in channel and channel pressure drops are evaluated by measuring the pressure inside the inlet and exit ports at different locations for different port dimensions. In these experiments, the measurement

Arabidopsis RADICAL-INDUCED CELL DEATH1 is involved in UV-B signaling.

The Arabidopsis radical-induced cell death1 (rcd1) mutant is sensitive to ozone fumigation and apoplastic superoxide, but tolerant to methyl viologen. In the present article, we report that the rcd1 mutant is also tolerant to supplementary UV-B radiation. The rcd1-1 mutant exhibits less accumulation of TT dimers, increased hypocotyl growth inhibition and higher accumulation of flavonoids under sup

Biomass Transportation

Extensive utilisation of logging residues, straw, and energy crops will lead to short transportation distances and thus low transportation costs. The average distance of transportation of biomass to a large-scale conversion plant, suitable for electricity or methanol production using 300 000 dry tonne biomass yearly, will be about 30 km in Sweden, if the conversion plant is located at the centre o

Interference of 7-hydroxymethotrexate with the determination of methotrexate in plasma samples from children with acute lymphoblastic leukemia employing routine clinical assays

The accuracy of two clinical assays, the enzyme-multiplied immunoassay (EMIT) and fluorescence polarization immunoassay (FPIA2), universally employed for measurement of plasma levels of methotrexate (MTX) in children administered a high dose of this drug for treatment of acute lymphoblastic leukemia was evaluated here. Because of its superior specificity, sensitivity, and precision, high performan

A study of the structure and metal tolerance of the soil microbial community six years after cessation of sewage sludge applications

Changes in soil microbial community structure and development of metal tolerance as a result of past applications of unamended sewage sludge and metal-amended sewage sludge were found in soils of a long-term field experiment at Braunschweig, Germany. Both the rate of sewage sludge application and metal amendment affected the size and activity of the microbial biomass and had caused changes in micr

Effect of the electrical currents generated by the intestinal smooth muscle layers on pancreatic enzyme activity: An in vitro study

Gut enzymes in the small intestine are exposed to extremely low electrical currents (ELEC) generated by the smooth muscle. In the present study, the in vitro tests were undertaken to evaluate the effect of these electric currents on the activity of the proteolytic pancreatic digestive enzymes. A simulator generating the typical electrical activity of pig gut was used for these studies. The electri

Her odours make him deaf: crossmodal modulation of olfaction and hearing in a male moth

All animals have to cope with sensory conflicts arising from simultaneous input of incongruent data to different sensory modalities. Nocturnal activity in moths includes mate-finding behaviour by odour detection and bat predator avoidance by acoustic detection. We studied male moths that were simultaneously exposed to female sex pheromones indicating the presence of a potential mate, and artificia

Cystatin C based peptidyl diazomethanes as cysteine proteinase inhibitors: Influence of the peptidyl chain length

The peptidyl diazomethanes Cbz-Gly-CHN2, Boc-Val-Gly-CHN2, H-Leu-Val-Gly-CHN2, Cbz-Leu-Val-Gly-CHN2 and Cbz-Arg-Leu-Val-Gly-CHN2, with peptidyl portions modelled after the proposed cysteine proteinase interacting N-terminal segment of human cystatin C, were synthesized. Their efficiency as cysteine proteinase inhibitors was tested against papain, human cathepsin B and bovine cathepsin B. All, exce

An experimental investigation of phytoplankton nutrient limitation in two contrasting low arctic lakes

We investigated whether phytoplankton communities in two lakes in SW Greenland were phosphorus or nitrogen limited. The study lakes have contrasting water chemistry (mean conductivities differ ten fold) and are located near Kangerlussuaq, SW Greenland (similar to 67 degrees N, 51 degrees W). A microcosm nutrient enrichment experiment was performed in June 2003 to determine whether nitrate or phosp

Gene-environment interactions in the aetiology of systemic lupus erythematosus

Systemic lupus erythematosus (SLE) is a disease that displays a multitude of symptoms and a vast array of autoantibodies. The disease course may vary substantially between patients. The current understanding of SLE aetiology includes environmental factors acting on a genetically prone individual during an undetermined time period resulting in autoimmunity and finally surpassing that individual's d

Neuropeptide Y and hypertension

Neuropeptide Y is a cotransmitter in the sympathetic nervous system with potent contractile effects on blood vessels. The plasma levels of neuropeptide Y-like immunoreactivity in patients with severe hypertension (> 120 mmHg) were increased compared with the levels in control subjects and were still elevated after long-term pharmacologic treatment of normotension. Neuropeptide Y stimulated DNA syn

Delta(9)-tetrahydrocannabinol and cannabinol activate capsaicin-sensitive sensory nerves via a CB1 and CB2 cannabinoid receptor-independent mechanism

Although Delta(9)-tetrahydrocannabinol (THC) produces analgesia, its effects on nociceptive primary afferents are unknown. These neurons participate not only in pain signaling but also in the local response to tissue injury. Here, we show that THC and cannabinol induce a CB1/CB2 cannabinoid receptor-independent release of calcitonin gene-related peptide from capsaicin-sensitive perivascular sensor

Light-induced linear dichroism in photoreversibly photochromic sensor pigments 1. Theory

With a photoreversibly photochromic regulator pigment such as phytochrome, linear action dichroism could theoretically be obtained after photoselection even if the molecules are initially randomly oriented. If randomly oriented Pr (red-absorbing phytochrome) (plant photoregulators) molecules are partially converted to Pfr (far-red absorbing phytochrome) molecules by plane-polarized red light, thos

Neurotransmitter release evoked by alpha-latrotoxin in the smooth muscle of the female pig urethra

Neuronal regulation of smooth muscle tone in the female pig urethra has mainly been studied in vitro using electrical field stimulation (EFS) of nerves. Excitatory control is considered to be exerted by released noradrenaline, whereas inhibitory control is non-adrenergic non-cholinergic (NANC), and mediated by nitric oxide (NO), and an as yet unidentified agent. We investigated the functional and

Deficient Fas expression by CD4+CCR5+T cells in multiple sclerosis

Objective: To evaluate whether T cells expressing CCR5 and CXCR3 from multiple sclerosis (MS) patients are more resistant to apoptosis. Methods: Expression of CD69, TNF-R1, Fas, FasL, bcl-2, and bax was investigated in 41 MS patients and 12 healthy controls by flow cytometry in CD4+ and CD8+ T cells expressing CCR5 and CXCR3. Results: In MS patients, the percentage of CD69 was increased and Fas ex

Scatter correction of transmission NIR spectra by photon migration data - Quantitative analysis of solids

The scope of this presentation is a new methodology to correct conventional NIR data for scattering effects. The technique aims at measuring the absorption coefficient of the samples rather than the total attenuation, measured by conventional NIR spectroscopy. The main advantage of this is that the absorption coefficient is independent of the path length of the light inside the sample, and therefo