Synthesis of Core@Brush microspheres by atom transfer radical polymerization for capturing phosphoprotein β-casein utilizing iron ion chelation and Schiff base bio-conjugation
This paper aims to design a highly efficient adsorbent to capture phosphoprotein in a facile way. One of the applications would be removing the allergenic β-casein from vaccines. To avoid the use of organic reagents and improve the capacity of immobilized metal ion affinity chromatography applied for bio-products at neutral pH, synergistic functional Core@Brush microspheres were designed utilizing