A New Immobilized NAD+ Analogue, Its Application in Affinity Chromatography and as a Functioning Coenzyme
Alkylation of NAD+ with iodoacetic acid followed by alkaline rearrangement gave N6-carboxymethyl-NAD+. Condensation of this analogue with 1,6-diaminohexane gave the analogue NAD+- N6-[N-(6-aminohexyl)-acetamide]. The coenzymic activity of the two derivatives was tested with malate dehydrogenase, alcohol dehydrogenase and lactate dehydrogenase. The efficiency relative to unsubstituted NAD+ was i
