Covalent attachment of heme to apocytochromes c in bacteria occurs on the outside of the cytoplasmic membrane and requires two reduced cysteinyls at the heme binding site. A constructed ResA-deficient Bacillus subtilis strain was found to lack c-type cytochromes. Cytochrome c synthesis was restored in the mutant by: (i) in trans expression of resA; (ii) deficiency in BdbD, a thioldisulfide oxidore

Musculoskeletal conditions often manifest with the onset of pain and the resulting physical limitations. Musculoskeletal pain is almost inevitable in in individual's lifetime. It is one of the most common reasons for self-medication and entry into the health care system. Musculoskeletal pain affects in 4 adults and is the Most commons Source of serious long-term pain and physical disability. The M

X-linked agammaglobulinemia (XLA) is a heritable immunodeficiency disorder that is caused by a differentiation block leading to almost complete absence of B lymphocytes and plasma cells. The affected protein is a cytoplasmic protein tyrosine kinase, Bruton's agammaglobulinemia tyrosine kinase (Btk). Btk along with Tec, Itk, Bmx and Txk belong to a distinct family of protein kinases. These proteins

There is an increasing interest in how oxidative stress can cause cells to go into apoptosis in both normal ageing and in neurodegenerative disorders. Previous research has implicated insulin-like growth factor-1 (IGF-1) as being involved in the pathogenesis in Alzheimer's disease (AD) by protecting the neurons through reducing neuronal susceptibility to oxidative stress. IGF-1 receptor (IGF-1R) p

Microchip immobilized enzyme reactors (mu IMERs) with immobilized endoglucanases were applied for the hydrolysis of methyl cellulose (MC). MCs of various molecular weights were hydrolyzed using two mu IMERs containing immobilized celloendoglucanase Cel 5A from Bacillus agaradhaerens (BaCel 5A) connected in series. Hydrolysis by the mu IMER could be confirmed from the average molar masses and molar

A species-wide phylogeographical study of the root vole (Microtus oeconomus ) was performed using the whole 1140 base pair mitochondrial (mt) cytochrome b gene. We examined 83 specimens from 52 localities resulting in 65 unique haplotypes. Our results demonstrate that the root vole is divided into four main mtDNA phylogenetic lineages that seem to have largely allopatric distributions. Net diverge

DNA damage in the form of cyclobutane pyrimidine dimers(CPDs) and (6-4) photoproducts(6-4PPs) induced by UV-B radiation in Arabidopsis thaliana at different temperatures was investigated using ELISA with specific monoclonal antibodies. CPDs and 64PPs increased during 3 h UV-B exposure, but further exposure led to decreases. Contrary to the commonly accepted view that DNA damage induced by UV-B rad

The suspension of the crystalline lens in the eye was studied in 11 species of teleost (bony fish) from 10 families and 7 orders by light and electron microscopy. In all species there were 4-5 ligaments in about the equatorial plane of the eye, in which also the tendon of the retractor lentis muscle attaches to the lens. In two cichlid species two additional ligaments were found running from the m

The aim of the paper is to examine the changing role of the state in employment regulation in an environment that has become more market-driven and Europeanised since the introduction of the European Monetary Union (EMU) and the European Employment Strategy (EES). The point of departure is a general discussion of the role of the state in capitalist development and a review on the recent debate on

A field study was carried out to evaluate the influence of N fertilization on the growth of the external mycelium of ectomycorrhizal (EM) fungi in a Norway spruce forest in SW Sweden. Nylon mesh bags filled with sand were buried in the soil for 6-18 months and the ingrowth of mycelium was used as an estimate of EM mycelial growth. Root-isolated, trenched plots were used to estimate background grow

A localized dehydration zone, Sondrum stone quarry, Halmstad, SW Sweden, consists of a central, 1 m wide granitic pegmatoid dyke, on either side of which extends a 2.5-3 m wide dehydration zone (650-700 degrees C; 800 MPa; orthopyroxene-clinopyroxene-biotite-amphibole-garnet) overprinting a local migmatized granitic gneiss (amphibole-biotite-garnet). Whole-rock chemistry indicates that dehydration

Lipid bilayers containing 5% nitrilotriacetic acid (NTA) lipids supported on SiO2 have been used as a template for immobilization of oligohistidine-tagged single-chained antibody fragments (scFvs) directed against cholera toxin. It was demonstrated that histidine-tagged scFvs could be equally efficiently coupled to an NTA-Ni2+-containing lipid bilayer from a purified sample as from an expression s

Potentially high human exposures to nanometer sized airborne particles occur due to welding and other thermal processes in industrial environments. Detailed field measurements of physical and chemical particle characteristics were performed in three work-shops in Sweden. Measurements were performed both in the plume 5-20 cm above the welding point and in the background air (more than 5 m away from

Aims/hypothesis To study, at a population level, school performance when leaving compulsory school of Swedish children whose mothers had diabetes during pregnancy compared with a reference population. Mehtohds We linked the Swedish Medical Birth Register with the Swedish School Mark Register, which contains school marks for all children in Sweden when leaving compulsory school. A total of 6,397 ch

We show that genomic-length DNA molecules imaged in nano-channels have an extension along the channel that scales linearly with the contour length of the polymer, in agreement with the scaling arguments developed by de Gennes for self-avoiding confined polymers. This fundamental relationship allows us to measure directly the contour length of single DNA molecules confined in the channels, and the

The stratospheric ozone layer has been depleted at high and mid-latitudes as a consequence of man's pollution of the atmosphere, and this results in increasing ultraviolet-B radiation at ground level. We investigate the effects of further radiation increases on plants and ecosystems by irradiating natural sub-Arctic and Arctic vegetation with artificial W-B radiation in field experiments extending

Background The study examined client factors of relevance in the establishment of helping alliance and in the prediction of dropout from a routine psychiatric setting admitting a variety of diagnoses and staffed with a multiprofessional team. Method Newly admitted patients (n=122) and staff completed questionnaires regarding helping alliance, and the patients also completed questionnaires regardin

BACKGROUND: Riks-Stroke, the Swedish national quality register on stroke care, provides unique opportunities to evaluate stroke units in routine clinical care. METHODS: Basic patient characteristics, process indicators and outcome variables are recorded in all 85 hospitals admitting acute stroke patients. A 3-month follow-up is included. RESULTS: There are wide variations between hospitals in the

Previous studies of high-risk breast cancer families have proposed that two major breast cancer-susceptibility genes, BRCA1 and BRCA2, may account for at least two-thirds of all hereditary breast cancer. We have screened index cases from 106 Scandinavian (mainly southern Swedish) breast cancer and breast-ovarian cancer families for germ-line mutations in all coding exons of the BRCA1 and BRCA2 gen