Normalizing to GADPH jeopardises correct quantification of gene expression in ovarian tumours-IPO8 and RPL4 are reliable reference genes
Background: To ensure a correct interpretation of results obtained with quantitative real-time reverse transcription-polymerase chain reaction (RT-qPCR), it is critical to normalize to a reference gene with stable mRNA expression in the tissue of interest. GADPH is widely used as a reference gene in ovarian tumour studies, although lacking tissue-specific stability. The aim of this study was to id