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Solubilization of enzymes in apolar solvents via noncovalent complex formation with soluble polymers
Triglyceride interesterification by lipases. 3. Alcoholysis of pure triglycerides
Lipase-catalyzed alcoholysis of triolein dissolved in ethanol or isopropanol for the formation of ethyl and isopropyl esters was investigated. Of 16 lipases screened, Amano lipase from P. fluorescens was selected for investigation of the effects of basic reaction conditions on alcoholysis yields. Ethanolysis yields were only slightly affected by water additions to immobilized lipase preparations.
Influence of solvent and water activity on kinetically controlled peptide synthesis
α-Chymotrypsin deposited on Celite was used to catalyse peptide synthesis reactions between N-protected amino acid esters and leucine amide in organic media with low water content. The influence of the solvent and the thermodynamic water activity on the reaction kinetics was studied. The substrate specificity in the reactions was shown to be a combination of the substrate specificity of the enzyme
Prevalence of dementia diagnosis not otherwise specified in eight European countries A cross-sectional cohort study
BackgroundDementia is a syndrome, with a wide range of symptoms. It is important to have a timely diagnosis during the disease course to reduce the risk of medication errors, enable future care planning for the patient and their relatives thereby optimizing quality of life (QoL). For this reason, it is important to avoid a diagnosis of dementia not otherwise specified (DNOS) and instead obtain a d
Organic solvent changes the chymotrypsin specificity with respect to nucleophiles
In α-chymotrypsin-catalyzed acyl-transfer reactions in water the specificity of the enzyme (the nucleophile reactivity of amino acid amides) is correlated with the substrate hydrophobicity and increases as the hydrophobicity of the side chain of the amino acid amides is increased. In a low water system (4% H2O) bulky amino acid amides are less efficient nucleophiles. The specificity of α-chymotryp
The association between knee confidence and muscle power, hop performance, and movement quality in people with anterior cruciate ligament injury at high risk of knee osteoarthritis
Activity and stability of horse‐liver alcohol dehydrogenase in sodium dioctylsulfosuccinate/cyclohexane reverse micelles
Horse liver alcohol dehydrogenase (EC 1.1.1.1) solubilized in sodium dioctylsulfosuccinate (AOT)/cyclohexane reverse micelles was used for the oxidation of ethanol and reduction of cyclohexanone in a coupled substrate/coenzyme recycling system. The activity of the enzyme was studied as a function of pH and water content. The enzyme was optimally active in microemulsions prepared with buffer of pH
Use of Perfluorochemicals for Oxygen Supply to Immobilized Cells
Elonei Mamre (e105), for cello
#105. Elonei Mamre, for cello
Illusions (e74), for ehn, cl, bsn, tpt, tbn, vla, vlc
This score is a 100% recomposition of my earlier piece illusions (#74/2006). As before, this music references and is inspired by a report titled, “Demonstrations of Auditory Illusions and Tricks” by Yoshitaka Nakajima, Takayuki Sasaki & Gert ten Hoopen.This piece is based on five classes of auditory illusions that were reported by Yoshitaka Nakajima, Takayuki Sasaki, & Gert ten Hoopen.
Validity and inter-rater reliability of an observational clinical test of assessing medio-lateral knee motion during a single-limb mini squat
Evaluating the safety benefit of retrofitting motorways section with barriers meeting a new EU standard : Comparison of observational before–after methodologies
The road safety barriers are today designed and installed in compliance with the European standards for Road Restraint Systems (EN 1317), which lays down common requirements for the testing and certification in all EU countries. The introduction of the European Union (EU) regulation for safety barriers, which is based on performance, has encouraged European road agencies to perform an upgrade of t
Modeling the water isotopes in Greenland precipitation 1959–2001 with the meso-scale model REMO-iso
Ice core studies have proved thed18O in Greenland precipitation to be correlated to thephase of the North Atlantic Oscillation (NAO). This subject has also been investigated inmodeling studies. However, these studies have either had severe biases in thed18O levels,or have not been designed to be compared directly with observations. In this study wenudge a meso‐scale climate model fitted with stabl
Oxidative DNA damage in human lymphocytes : Correlations with plasma levels of α-tocopherol and carotenoids
In order to investigate whether oxidative damage is associated with differences in antioxidant intake, we measured the levels of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) in lymphocytes and α-tocopherol and several carotenoids in plasma of women with different dietary habits. We found that women from Granada (Spain), a region with a typically Mediterranean diet, had significantly higher leve
Partiklar i atmosfären påverkar klimatet
Partiklar i atmosfären – aerosoler – har en avkylande effekt på jordens klimat. Aerosolernas sammanlagda effekt bedöms i IPCCs femte stora rapport, AR5, som något mindre än vad som rapporterades i 2007 i AR4. Partiklars avkylande effekt har troligen i viss mån motverkat växthusgasutsläppens uppvärmande påverkan. Vi kan dock inte förlita oss på sådan draghjälp i klimatarbetet. Internationella övere
Serum tau fragments as predictors of death or poor neurological outcome after out-of-hospital cardiac arrest
Background: Anoxic brain injury is the primary cause of death after resuscitation from out-of-hospital cardiac arrest (OHCA) and prognostication is challenging. The aim of this study was to evaluate the potential of two fragments of tau as serum biomarkers for neurological outcome. Methods: Single-center sub-study of 171 patients included in the Target Temperature Management (TTM) Trial randomly a
Homo Pictor Redux : The Cognitive Semiotics of Temporally and/or Spatially Distant Objects
Lipases have similar water activity profiles in different reactions
The shape of the profiles of enzyme activity versus water activity for four different lipases were independent of the reaction used to determine the activity. The profile for each lipase (Rhizopus arrhizus, Pseudomonas sp., Candida rugosa and Lipozyme) in esterification, hydrolysis and transesterifications profiles were the same. In transesterification the yield was unaffected by the water activit