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Although calmodulin (CaM) is commonly considered to be an intracellular protein, it has been suggested lately that it is released and exerts functions extracellularly. In the present investigation this was studied in in vitro regenerating adult frog (Rana temporaria) sciatic nerves. Using a multi‐compartment incubation chamber, the non‐neuronal cells in the outgrowth region of such nerves were rad
Nerve growth factor stimulated axonal outgrowth from explanted mouse dorsal root ganglia is dependent on mitogen activated protein kinase. PD98059 ([2-(2'amino-3'-methoxyphenyl)-oxanaphthalen-4-one]) blocks mitogen activated protein kinase by inhibiting its immediate upstream activator, mitogen activated protein kinase kinase (also known as MEK). Here we used PD98059 to study the role of mitogen a
Abstract: The release of radiolabeled material from regenerating frog sciatic nerves was studied using a multicom‐ partment chamber, in which the ganglia and the outgrowth region, respectively, were separated from the rest of the nerve. The nerves were incubated with radioactive amino acids in the ganglionic compartment, and the material transported to and released at the outgrowth region was coll
The adult frog dorsal root ganglia (DRGs) and their sciatic nerves (ScN) survive in organ culture for several days. About 3 days after a local test crush, the sensory axons start to regenerate into the distal nerve stump at a rate of approximately 0.6-0.9 mm/day. The axonal outgrowth is inhibited in a non-toxic way by low concentrations of three different phospholipase A2 (PLA2) inhibitors: 4-brom
Dorsal root ganglia (L4 and L5) with attached spinal roots and nerve stumps were isolated from young adult mice and cultured in a layer of extracellular matrix material (matrigel). Within one day, a large number of axons grew out from the cut ends of the nerve and the dorsal root. The average outgrowth length was more than doubled by nerve growth factor, which also strongly increased the number of
The adult frog dorsal root ganglia (DRG) together with the sciatic nerve (ScN) has previously been shown to survive in organ culture for several days. If a local test crush is made at the beginning of culturing, there is an initial delay of about 3 days before the sensory axons start to grow into the distal nerve stump at a rate of about 0.6-0.9 mm/day. The present results showed that axonal growt
The sensory axons of the adult mouse sciatic nerve were shown to regenerate after a local test crush lesion in vitro in a serum-free medium. The average outgrowth distance of the leading axons after culturing for 3 days was 2.8 ± 0.1 mm, which was shorter than in vivo (3.8 ± 0.2 mm). With the use of a compartmentalised culture system we could show that regeneration was partially dependent on local
We have studied the role of protein kinase C (PKC) in peripheral nerve regeneration by using the cultured adult mouse sciatic nerve, which displays regrowth of sensory axons under serum-free conditions. By the use of immunohistochemistry we show that one of the isoforms of PKC, PKCβ, is present in the nerve cell bodies of normal nerves and is upregulated after injury. In spite of this, the specifi
Retinitis pigmentosa is a group of inherited retinal degenerative diseases characterized by the loss of photoreceptors and vision for which no effective treatment is available. Several animal models of retinitis pigmentosa are used to elucidate its pathogenesis and to devise therapies. The retinal degeneration (rd1) mouse is one such animal model in which rod-specific phosphodiesterase (PDE) is in
The effects of glial cell line-derived neurotrophic factor on axonal outgrowth and apoptosis were studied in vitro using explanted dorsal root ganglia-peripheral nerve preparations of adult mice. In gels of matrigel or collagen type 1, glial cell line-derived neurotrophic factor increased both the numbers and lengths of axons growing out of explanted preparations, although less effectively than ne
Recent literature has suggested how late-medieval families may have used financial markets to navigate the life cycle. Precious little is known about the precise connections between the life cycle and family on the one hand and investments in financial instruments on the other, though. We analyse late-medieval investment behaviour using a new dataset of hundreds of life annuities. Our data give ag
Recent changes in user employment of Internet based services, new deployment technologies for mobile networks as well as an ongoing realisation of fixed and mobile converged networks e.g. the EU FP7 project COMBO, are significant examples of enablers for increasing demands on DSL links. Investigating cross-layer dependencies between all layers in the OSI reference model becomes increasingly import
Companies adopt different rationales for purchasing in order to make production processes as efficient as possible. This paper presents the results of an investigation into how purchasing strategies can vary between companies and countries. Two industrialised building companies, one in Sweden and one in Australia, were the objects of study. The purpose was to compare and understand the rationale b
The purchasing strategies in the construction industry have been considered by many writers in construction management as short-term and arms-length. However, a different picture is portrayed in the manufacturing industry, where the purchasing strategies are mostly long-term to secure supply for production. Industrialized building is at the crossroad between construction and manufacturing, which rThe purchasing strategies in the construction industry have been considered by many writers in construction management as short-term and arms-length. However, a different picture is portrayed in the manufacturing industry, where the purchasing strategies are mostly long-term to secure supply for production. Industrialized building is at the crossroad between construction and manufacturing, which r
Retinitis pigmentosa (RP), an inherited blinding disease, is caused by a variety of different mutations that affect retinal photoreceptor function and survival. So far there is neither effective treatment nor cure. We have previously shown that poly(ADP-ribose)polymerase (PARP) acts as a common and critical denominator of cell death in photoreceptors, qualifying it as a potential target for future
In economic and management literature, the relationship between supplier and buyer can be more or less intimate. It can vary from market-driven with a constant change of suppliers to a value-driven relationship with one sole supplier. Purchasing strategies of construction companies have often been described as short-sighted, where price is the most considered aspect. Recent lean management literat
Cellulose is insoluble in water but can be dissolved in strong acidic or alkaline conditions. How well dissolved cellulose is in solution and how it organizes are key questions often neglected in literature. The typical low pH required for dissolving cellulose in acidic solvents limits the use of typical characterization techniques. In this respect, Polarization Transfer Solid State NMR (PT ssNMR)
There is a great need to identify new and better prognostic and predictive biomarkers to stratify prostate cancer patients for optimal treatment. The aims of this study were to characterize the expression profile of pre-B cell leukemia homeobox (PBX) transcription factors in prostate cancer with an emphasis on investigating whether PBX3 harbours any prognostic value. The expression profile of PBX3
Recent studies indicate that patients experience pain after day surgery for a longer period than previously known. This requires verification. This was a prospective, descriptive correlational study. A convenience sample of 298 day surgery patients undergoing various surgical procedures was asked to report pain intensity and its interference with daily function 48 hours, seven days and three month
