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Persistent Aspergillus fumigatus infection in cystic fibrosis : impact on lung function and role of treatment of asymptomatic colonization-a registry-based case-control study

BACKGROUND: Aspergillus fumigatus is the most common filamentous fungus isolated from the airways of people with cystic fibrosis (CF). The aim of this study was to investigate how chronic A. fumigatus colonization affects lung function in people with CF, to identify risk factors for colonization, and to evaluate antifungal treatment of asymptomatic Aspergillus colonization.METHODS: Data from 2014-

Genetic regulation of RNA splicing in human pancreatic islets

BackgroundNon-coding genetic variants that influence gene transcription in pancreatic islets play a major role in the susceptibility to type 2 diabetes (T2D), and likely also contribute to type 1 diabetes (T1D) risk. For many loci, however, the mechanisms through which non-coding variants influence diabetes susceptibility are unknown.ResultsWe examine splicing QTLs (sQTLs) in pancreatic islets fro

The genetic contribution to hand osteoarthritis

Objective: To estimate the genetic contribution to doctor-diagnosed hand osteoarthritis (OA). Methods: Using data from the Swedish Twin Registry and National Patient Register, we conducted a 20-year population-based longitudinal cohort study including 59,970 twins aged 35 years or older. We studied inpatient and outpatient doctor-diagnosed hand OA using ICD-10 codes from 1997 until 2016, including

High-resolution HLA class II sequencing of Swedish multiple sclerosis patients

Multiple sclerosis (MS) is a chronic neurological disease believed to be caused by autoimmune pathogenesis. The aetiology is likely explained by a complex interplay between inherited and environmental factors. Genetic investigations into MS have been conducted for over 50 years, yielding >100 associations to date. Globally, the strongest linkage is with the human leukocyte antigen (HLA) HLA-DRB5*0

What constitutes traffic event severity in terms of human danger perception?

This study focuses on the severity gradation of non-collision events. Earlier theoretical work has suggested that a proper severity measure for an event should reflect the risk of personal injury, which is often split into two components, including the risk of a collision and the potential consequences had the collision taken place. While a great number of severity measures have been suggested, mo

Biomass burning emissions and influence of combustion variables in the cone-calorimeter

Emissions from biomass burning are highly variable and depend on combustion conditions as well as fuel properties. Simultaneous emissions from pyrolysis, smouldering, and combustion of the biomass material(s) burning leads to uncertainties in how these processes contribute to emissions of individual or groups of compounds as well as to total particle emissions. These uncertainties are difficult to

Population-Based Cohort Study from a Prospective National Registry : Better Long-Term Survival in Esophageal Cancer After Minimally Invasive Compared with Open Transthoracic Esophagectomy

BACKGROUND: Recent research indicates long-term survival benefits of minimally invasive esophagectomy (MIE) compared with open esophagectomy (OE) for patients with esophageal and gastroesophageal junction (GEJ) cancers, but there is a need for more population-based studies.METHODS: We conducted a prospective population-based nationwide cohort study including all patients in Sweden diagnosed with e

Modal analysis of CLT beams: Measurements and predictive simulations

Cross-laminated timber (CLT) as a prime example of an innovative product within the field of civil engineering has over the recent years attracted attention of the construction industry and engineers around the world. As a natural composite with different orientation of layers in addition to anisotropic properties of wood, CLT can be a challenging material in predictive numerical modelling. This i

Long-Short Term Memory Technique for Monthly Rainfall Prediction in Thale Sap Songkhla River Basin, Thailand

Rainfall is a primary factor for agricultural production, especially in a rainfed agricultural region. Its accurate prediction is therefore vital for planning and managing farmers’ plantations. Rainfall plays an important role in the symmetry of the water cycle, and many hydrological models use rainfall as one of their components. This paper aimed to investigate the applicability of six machine le

Attosecond dynamics of multi-channel single photon ionization

Photoionization of atoms and molecules is one of the fastest processes in nature. The understanding of the ultrafast temporal dynamics of this process often requires the characterization of the different angular momentum channels over a broad energy range. Using a two-photon interferometry technique based on extreme ultraviolet and infrared ultrashort pulses, we measure the phase and amplitude of

Aerosol formation and emissions from realistic compartment fires

Firefighters are occupationally exposed to a large number of toxic compounds (IARC 2010). The occupational exposure of firefighters has been classified as potentially carcinogenic (class 2B, IARC; (Straif K. et al. 2007)). Poorly quantified emission factors and low understanding of when various aerosol emissions are likely to form during a fire event (initiation, combustion, extinguishing) inhibit

Survival trends of patients with non-metastatic gastric adenocarcinoma in the US and European countries : the impact of decreasing resection rates

BACKGROUND: We previously observed decreasing resection rates of non-metastatic gastric adenocarcinoma (GaC) in the US and some European countries. If and to what extent these trends affect the trends in overall survival (OS) of patients with non-metastatic GaC at the population level remain unclear. This large international population-based cohort study aimed to assess the impact of the previousl

A low-cost and open-source protocol to produce key enzymes for molecular detection assays

Here, we describe a detailed step-by-step protocol for the expression, purification, quantification, and activity determination of key enzymes for molecular detection of pathogens. Based on previous reports, we optimized the protocol for LbCas12a, Taq DNA polymerase, M-MLV reverse transcriptase, and TEV protease to make it compatible with minimal laboratory equipment, broadly available in low- and

Monitoring mental distress in Para athletes in preparation, during and after the Beijing Paralympic Games 2022: A 22 week prospective mixed-method study

It is common in elite sport to monitor athletes' training load, injuries and illnesses, but mental distress is rarely included. An improved understanding of the epidemiology of mental distress among elite Para athletes and how their coaches perceive such monitoring would allow us to better develop and implement preventive measures. The purpose of this study was therefore to (1) prospectively descr