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Monitoring the oxidation of docosahexaenoic acid in lipids

The oxidation of free DHA, DHA mixed with PC, and DHA incorporated into PC, PE, or TG was evaluated to determine which lipid provided DHA with the best protection against oxidation. DHA was either situated at the sn-1 position, sn-2 position, or both positions of the phospholipid, whereas the TG contained DHA at all positions. All lipids were incubated as bulk lipids, in chloroform, or as an emuls

Assessment of CAR- or CD46-Dependent Adenoviral Vector-Mediated TRAIL Gene Therapy in Clinical Adenocarcinoma Lung Cancer Cells

Adenoviral vector-mediated transfer of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) can be a powerful approach to lung cancer therapy. However, the efficiency of adenoviral vector gene transfer and the sensitivity to TRAIL-induced apoptosis in the context of adenoviral vector gene transfer have yet to be characterized in primary lung cancers. In this study, we investigated the e

Favoured owners, neglected tenants: Privatisation of state owned housing in Hanoi

This paper presents selected findings from the project 'Privatisation of public housing in Hanoi, the impacts on housing quality and social equality' which was financed by the Swedish International Development Authority (SIDA). The project analyses the consequences of privatisation on the housing conditions of those tenants and owners who live in privatised and partly privatised multi family apart

Additions to the spectrum and energy levels and critical compilation of doubly ionized boron, BIII

We have undertaken the study of the Li-like spectrum of doubly ionized boron, B III. The spectroscopic data have been obtained with beam-foil spectroscopy and high-resolution spark spectroscopy. The experimental work was combined with theoretical calculations using ab initio and semi-empirical techniques. About 50 new transitions have been observed, and most of the previously known lines have been

Experimental investigation of local heat transfer in a square duct with various-shaped ribs

In the present study, experimental studies are carried out to investigate the heat transfer and friction characteristics in a square duct roughened by various-shaped ribs on one wall. The ribs are oriented transversely to the main stream in a periodic arrangement. Liquid crystal thermography is employed to measure the local and average heat transfer coefficient on the ribbed surface. The rib heigh

Fifteen-year risk of major coronary events predicted by Holter ST-monitoring in asymptomatic middle-aged men.

Background: Ambulatory electrocardiogram monitoring (Holter) with ST-analysis as a measure of myocardial ichemia has in populations with coronary heart disease been shown to predict major coronary events: death, myocardial infarction or coronary revascularization. There has, however, been conflicting evidence regarding the usefulness of this technique in identification of healthy subjects with inc

The non-oxidative pentose phosphate pathway controls the fermentation rate of xylulose but not of xylose in Saccharomyces cerevisiae TMB3001

Saccharomyces cerevisiae is able to ferment xylose, when engineered with the enzymes xylose reductase (XYL1) and xylitol dehydrogenase (XYL2). However, xylose fermentation is one to two orders of magnitude slower than glucose fermentation. S. cerevisiae has been proposed to have an insufficient capacity of the non-oxidative pentose phosphate pathway (PPP) for rapid xylose fermentation. Strains ove

Monitoring the bioavailability of FEIBA with a thrombin generation assay

Background: Hemophilia A patients with inhibitors are generally treated with preparations containing activated coagulation factors to achieve hemostasis by bypassing factor (F)VIII. Objectives: We developed an assay for monitoring the kinetic of thrombin generation in human FVIII inhibitor plasma reconstituted in vitro with activated prothrombin complex concentrate, FEIBA, and in plasma samples fr

Room-temperature semiconductor heterostructure refrigeration

With the proper design of semiconductor tunneling barrier structures, we can inject low-energy electrons via resonant tunneling, and take out high-energy electrons via a thermionic process. This is the operation principle of our semiconductor heterostructure refrigerator (SHR) without the need of applying a temperature gradient across the device. Even for the bad thermoelectric material AlGaAs, ou

Early stages of Gondwana breakup: The Ar-40/Ar-39 geochronology of Jurassic basaltic rocks from western Dronning Maud Land, Antarctica, and implications for the timing of magmatic and hydrothermal events

[1] The timing of magmatic events forming Jurassic basaltic rocks in Dronning Maud Land (DML), Antarctica, and hydrothermal activity that affected them is addressed with detailed Ar-40/Ar-39 incremental heating dating of feldspars. Plagioclase from an Utpostane gabbro and from a Kirwanveggen dolerite dike yield indistinguishable plateau ages at 177 +/- 1.8 Ma. Because of geologic controls, this es

Enhanced Exopolysaccharide Production by Metabolic Engineering of Streptococcus thermophilus.

It is possible that the low levels of production of exopolysaccharides (EPSs) by lactic acid bacteria could be improved by altering the levels of enzymes in the central metabolism that influence the production of precursor nucleotide sugars. To test this hypothesis, we identified and cloned the galU gene, which codes for UDP glucose pyrophosphorylase (GalU) in Streptococcus thermophilus LY03. Homo

Asymptotic distribution of Mann's test for trend for m-dependent seasonal observations

Mann proposed a non-parametric test for trend for independent non-seasonal observations. In this paper we generalize the test to m-dependent seasonal observations. Furthermore, an estimator of trend, based on Kendall's tau, is studied and its asymptotic distribution is proved to be normal. The results are exemplified by a moving average process.

A general approach to the apparent permeability index

The apparent permeability index is widely used as part of a general screening process to study drug absorption, and is routinely obtained from in vitro or ex vivo experiments. A classical example, widely used in the pharmaceutical industry, is the in vitro Caco-2 cell culture model. The index is defined as the initial flux of compound through the membrane (normalized by membrane surface area and d

Apoptosis and proliferation of dentate gyrus neurons after single and intermittent limbic seizures

Neuronal apoptosis was observed in the rat dentate gyrus in two experimental models of human limbic epilepsy. Five hours after one hippocampal kindling stimulation, a marked increase of in situ terminal deoxynucleotidyltransferase-mediated dUTP nick-end labeling (TUNEL) of fragmented DNA was observed in nuclei located within and on the hilar border of the granule cell layer and in the polymorphic

Normalization of hemoglobin level in patients with chronic kidney disease and anemia

Background: Whether correction of anemia in patients with stage 3 or 4 chronic kidney disease improves cardiovascular outcomes is not established. Methods: We randomly assigned 603 patients with an estimated glomerular filtration rate (GFR) of 15.0 to 35.0 ml per minute per 1.73 m(sup 2) of body-surface area and mild-to-moderate anemia (hemoglobin level, 11.0 to 12.5 g per deciliter) to a target h

Isolation of potato proteins using simulated moving bed technology.

The simulated moving bed (SMB) concept of chromatography was applied to treat potato juice from production of starch. The aim was to harvest proteins. SMB offers possibilities to operate with different process strategies and in this study it was shown possible to harvest up to 80% of the protein in a process utilizing very little extra water besides that already present in the juice. After depleti