The major inhibitor of the cysteine class of proteinases found in human body fluids, such as spinal fluid, milk, and seminal plasma, is cystatin C. In this study we show that human bronchoalveolar fluid also contains cystatin C and examine cystatin C expression by alveolar macrophages in vitro. Immunoprecipitation of extracts of metabolically labeled cells and immunoblotting of cellular extracts a

SPECIES-SPECIFIC TRACKS of animals can be an effective way of mapping species that are hard to find even if they are present. We used observations of sap rows on trees to calculate densities of Eurasian Three-toed Woodpeckers Picoides tridactylus. We surveyed 14 fixed routes in northern Sweden below the montane forest for sap rows during the autumn of 2020. We used our observations of fresh sap ro

Protein D, a novel surface protein of the bacterial species Haemophilus influenzae with affinity for human IgD, was isolated after solubilization with sonication and Sarcosyl-extraction by a single SDS-PAGE step. From 1 ml of packed bacteria was prepared 0.25 mg of purified protein D. The apparent m.w. of protein D was estimated to 42,000 by SDS-PAGE and gel chromatography. Edman degradation cycle

Increases in phenotypic variation under extreme (e.g. novel or stressful) environmental conditions are emerging as a crucial process through which evolutionary adaptation can occur. Lack of prior stabilizing selection, as well as potential instability of developmental processes in these environments, may lead to a release of phenotypic variation that can have important evolutionary consequences. A

Cystatin C, a potent inhibitor of cysteine proteases such as papain and cathepsin B, was examined for its effect on human coronaviruses OC43 and 229e. Both viruses were greater than 99% inhibited by 0.1 mM inhibitor. Endpoint titrations showed that inhibiting activity paralleled that of leupeptin, a serine and cysteine protease inhibitor, and indicated that 1 to 2 microM inhibitor, slightly above

In circumpolar regions, coastlines offer rich constellations of diverse resources, and have long been a focus of human habitation. Despite the rich archaeological records that are located along many northern coastlines, there is a relatively limited understanding of the range of factors that informed local settlement strategies. Northern Norway has one of the world’s longest and best-preserved arc

The unsurpassed versatility of heat sensitive biosensors is illustrated by various new applications of the flow-calorimetric biosensor system, the enzyme thermistor (ET) developed in our laboratory. The ET measures the reaction heat in a small column containing immobilized biocatalyst. Since most biological reactions are exothermic, the ET has a very broad applicability and numerous assays of clin

We present an overview of the 4MOST project at the Preliminary Design Review. 4MOST is a major new wide-field, high-multiplex spectroscopic survey facility under development for the VISTA telescope of ESO. 4MOST has a broad range of science goals ranging from Galactic Archaeology and stellar physics to the high-energy physics, galaxy evolution, and cosmology. Starting in 2021, 4MOST will deploy 24

The heat production by human plasma in healthy and uraemic subjects has been measured by direct isothermal microcalorimetry. The plasma from uraemic subjects displayed an increased heat production compared to that of normal plasma. The heat production by plasma from healthy subjects, but not by that from uraemic patients, was both proportional to the amount of thiol groups in the plasma and also t

Aqueous coordination of cyanide and verification of the composition of the resulting coordination compound is important to ensure confidence in its coordination. Isothiocyanato compounds 1–7 were synthesized employing different synthetic routes and the complex, [Mo2O2(μ-S)2(SCN)5(CH3CN)]3−, 4, was structurally characterized. Ligand exchange reaction of 4 with cyanide forms 8, a cyano complex. Comp

Dopaminergic treatment in combination with rehabilitative training enhances long-term recovery after stroke. However, the underlying mechanisms on structural plasticity are unknown. Here, we show an increased dopaminergic innervation of the ischemic territory during the first week after stroke induced in Wistar rats subjected to transient occlusion of the middle cerebral ar-tery (tMCAO) for 120 mi

Background/Aims. Obesity is a well-established risk factor for the development of numerous chronic diseases. However, there is a small proportion of obese individuals that seem to escape these aforementioned conditions—Metabolically Healthy Obesity (MHO). Our aim was to do a metabolic and biomarker profiling of MHO individuals. Method. Associations between different biomarkers (proteomics, lipidom

In this paper, a novel multi-region Eulerian model is presented to study the conversion of thermally thick biomass particles. In the present model, the particle and the surrounding fluid are treated as two separate regions that are coupled through an interface. There are some benefits for using a multi-region approach, including a more realistic radiation modeling, flexibility to define the flow b

BACKGROUND: The COVID-19 pandemic has had a profound effect on the emotional well-being of expecting mothers. Sweden's unique strategy for managing COVID-19 involved no national lockdown. Emphasis was instead placed on limiting crowding and asking citizens to practice social distancing measures.AIM: To gain a deeper understanding of how women not infected by SARS-CoV-2 experienced pregnancy during

We analyze to what extent the contribution of banks to systemic risk depends on their centrality in financial networks. We find that centrality is an important determinant of systemic risk, but not primarily by its direct effect. Its main influence is to make other risk measures, such as probability of default, more important for highly connected banks. Neglecting the indirect effect of centrality

Given the gaps between EU ambitions regarding energy community development and the current reality of clean energy communities in Europe, we explore a research framework enabling viable multi- and interdisciplinary research into new clean energy communities. We offer a definition of new clean energy communities, discuss their potential for wider dissemination and identify four factors that contrib