Neuroblastoma is one of the most common paediatric malignancies. Detection of somatic genetic alterations in this tumour is instrumental for its risk stratification and treatment. On the other hand, an absence of detected chromosomal imbalances in neuroblastoma biopsies is difficult to interpret because it is unclear whether this situation truly reflects the tumour genome or if it is due to subopt

https://antroperspektiv.org/2018/10/15/minnen-av-det-forlorade-och-trosten-i-ting-under-palestinsk-exil/#more-687

Hypothermic treatment after birth asphyxia was introduced as a recommended practice in Sweden in 2007. CPUP is a national quality register and surveillance program that encompasses the total population of children with cerebral palsy (CP) in Sweden. In an analysis of CPUP data children with CP and asphyxia treated with cooling were compared to children with CP and asphyxia who were not cooled. A l

Background: Overnight fasting is often prolonged before scheduled surgery, and the extent of perioperative fluid replacement may influence outcome. In clinical practice, basic requirements are estimated at 1.2-2.0 mL·kg−1·h−1, but there is little contemporary clinical data on what deficits result from complete fasting. This prospective preclinical study was designed to determine total fluid loss d

Metastatic breast cancer is responsible for most breast cancer-related deaths. Disseminated cancer cells have developed an intrinsic ability to resist anchorage-dependent apoptosis (anoikis). Anoikis is caused by the absence of cellular adhesion, a process that underpins lumen formation and maintenance during mammary gland development and homeostasis. In healthy cells, anoikis is mostly governed b

Just like current software systems, models are characterised by increasing complexity and rate of change. Yet, these models only become useful if they can be continuously evaluated and validated. To achieve sufficiently low response times for large models, incremental analysis is required. Reference Attribute Grammars (RAGs) offer mechanisms to perform an incremental analysis efficiently using dyn

We have developed an assay, based on nanofluidic channels and fluorescence microscopy, for optical mapping of DNA based on competitive binding between two molecules - one fluorescent and one sequence selective. From the experimental data we can extract binding constants for the two competing DNA binders, which may be subsequently used to calculate a theoretical reference map of any DNA with known

To better understand how deformable and non-spherical particles behave in sorting devices based on deterministic lateral displacement we generate models of biological particles with tunable size, shape and mechanical properties using stop-flow lithography and we explore how these parameters play a role in our separation devices. Hollow and solid cylinders are compared with respect to their deforma

A new chip design for handling and studying chromosomal DNA is described. Folding the nanochannels enables us to image an entire yeast chromosome in a single field of view. Together with existing mapping techniques our new design opens up the possibility of directly analyzing entire chromosomes in a single field of view using fluorescence microscopy. This would enable, for example, identification

How good is your best chimpanzee impression? Go to the zoo and you probably wouldn’t be surprised to see people copying chimpanzees in order to grab their attention. But our latest research, which recently won the Ig Nobel Prize for Anthropology, suggests you are just as likely to see chimpanzees imitating the human visitors.

Morphology represents a hitherto unexploited source of specificity in microfluidic particle separation and may serve as the basis for label-free particle fractionation. There is a wealth of morphological changes in blood cells due to a wide range of clinical conditions, diseases, medication and other factors. Also, blood-borne parasites differ in morphology from blood cells. We present the use of

We demonstrate fluidics realized in thin film plastic foils patterned using roll-toroll nanoimprinting lithography (rrNIL). Realizing fluidics devices in thin plastic foils opens up for parallel operation in stacked devices. It also provides a convenient format for storage and distribution of the devices.

We present experimental results and simulations on a simple method for tunable particle separation based on a combination of Deterministic Lateral Displacement (DLD) and Insulator Based Dielectrophoresis (I-DEP). Rather than deriving its tunability from its elastic properties[1], our present device uses an applied AC field to perturb the particle trajectories in the pressure-driven flow and is the

We demonstrate direct visualization of melting mapping of DNA stretched in nanoscale channels[1] using standard staining methods and epifluorescence microscopy to gain information on the local AT/GC ratio along large DNA molecules. Our development opens up a novel route to mapping of large-scale genomic variations.

In this work, the near-field and far-field electric magnetic (EM) wave distributions of metallic slits was observed using tapered fiber probe and modelled using finite difference time domain (FDTD) computer simulations. The EM wave field distribution from rectangular slits with widths 100 nm, 300 nm, and 500 nm was mapped with excitation wavelength λ = 532 nm. λ/2 can be considered a characteristi

Chip based bio/chemical analysis relies on networks of fluidic channels that are connected to reaction chambers and sensors. For sensitive detection it is important to scale down the size of the channels so that they approach the relevant length scales of the molecules of interest. Here we have made sealed channels on the 100 nm scale using nanoimprinting to pattern the sacrificial polymer polynor

Object. The pathogenesis of cerebrospinal fluid (CSF) shunt infection is characterized by staphylococcal adhesion to the polymeric surface of the shunt catheter. Proteins from the CSF-fibronectin, vitronectin, and fibrinogen-are adsorbed to the surface of the catheter immediately after insertion. These proteins can interfere with the biological systems of the host and mediate staphylococcal adhesi

As the tip in the atomic force microscope is scanned over the sample surface an image results which contains information from the sample as well as from the tip. This mainly results in an increase of the apparent size of the sample. If the tip is reasonably sharp the contribution from the tip is small. In some cases the widening still persists in spite of a very sharp tip. In this letter, a model

In this study we report on the first direct observation of the real shape of the atomic force microscopy (AFM) tip using the AFM technique itself, utilizing a specially designed sample geometry. This is the first report of the so-called inverse AFM mode in which the tip is actually used as the sample and vice versa. We propose how this method can, with a very high accuracy, be used for studying ob