Human MoAbs ofIgM class were developed against three regions of the HIV-1 envelope. Uninfected donor lymphocytes were immunized in vitro with recombinant protein pB1. Four out of five antibodies were directed to different parts of the V3 region, which contains a major neutralizing site. Two out of these antibodies were directed to more than one amino acid sequence, indicating reactivity to discont

Neisseria meningitidis is a causative agent of meningitis. Despite vaccination programs, it still causes a large number of deaths in young children. Early diagnosis followed by passive immunization with human monoclonal antibodies could be an approach to effective therapy. Peripheral blood lymphocytes from normal, healthy blood donors and from vaccinated individuals were immunized in vitro, using

The relation between domain structures of recombinant monoclonal antibody fragments and their reaction kinetics was studied for the first time using a novel biosensor based on surface plasmon resonance technology. The association and dissociation rate constants of Fab, Fv and single domain (VH fragment) anti-lysozyme antibodies were determined and compared to the intact monoclonal antibody. Fab an

Human x mouse hybridoma cells were grown in dialysis tubing (DT) to obtain large quantities of human monoclonal antibodies (MAb). Hybridoma cells were grown inside the DT, which was placed in a tissue culture flask containing medium. The medium inside the DT was supplemented with different additives which may be selected depending on the intended use of the MAb. About 10-50 times higher concentrat

The safety and pharmacokinetics of carbetocin, a long-acting oxytocin analogue, were studied in 25 healthy nonpregnant women. The distribution and elimination half-lives of a 0.4-mg intravenous dose were found to be 5.5 ± 1.6 minutes and 41 ± 11.9 minutes, respectively. Similarly, the half-lives of a 0.8-mg intravenous dose were found to be 6.1 ± 1.2 minutes and 42.7 ± 10.6 minutes. Approximately

Vid biblioteken i Burlöv och Ängelholm har tidigare bedrivits projekt att främja folkbibliotekens utveckling mot kulturhus (Kulturnämnden 2012). Arbetet med projekten, Kulturhusprojektet i Arlöv och Kulturstråket i Ängelholm, följdes i en studie av författaren och har rapporterats tidi-gare (Rivano Eckerdal 2013). Föreliggande rapport är resultatet av en uppföljande studie som be-dri¬vits under år

This paper is based on the keynote talk that I gave on the EAHIL+ICAHIS+ICLC 2015 Workshop in Edinburgh. A starting point for the talk was the theme for the Workshop: “Research-minded: supporting, understanding, conducting research”. Some features of qualitative research are presented and a number of methods are mentioned before zooming in on interviewing, discussing some aspects of that method wiThis paper is based on the keynote talk that I gave on the EAHIL+ICAHIS+ICLC 2015 Workshop in Edinburgh. A starting point for the talk was the theme for the Workshop: “Research-minded: supporting, understanding, conducting research”. Some features of qualitative research are presented and a number of methodsare mentioned before zooming in on interviewing, discussing some aspects of that method wit

Heat transfer in gas turbine related applications is investigated by steady state liquid crystal thermography (LCT) in this thesis. The work presented includes outlet guide vane heat transfer and gas turbine internal cooling.The heat transfer on the outlet guide vane (OGV) surface and its endwall region is investigated experimentally in a linear cascade test facility. The inlet flow angle is varie

Sammanfattning av eget föredrag om tidningen Lundagårds symboldjur "Quatten" (hållet för Sällskapet Pelle Svanslös Vänner i Uppsala 2015-11-16)

PURPOSE: The purpose of this work was to develop a new family of test algorithms for computerized static threshold perimetry which significantly reduces test time without any reduction of data quality.METHODS: A comprehensive visual field model constructed from available knowledge of normal and glaucomatous visual fields is continuously updated during testing. The model produces threshold estimate

BACKGROUND: High reproducibility of test measurements is often considered an indication of high reliability of test results. The aim of the current study was to analyse the role of the traditional perimetric reliability indices, False Negative and False Positive responses, and Fixation Losses, as indicators of test reliability in comparison with threshold reproducibility in patients with suspect o

Objective: To acquire a deepened understanding of how stigma in healthcare affects health seeking behaviours of same-sex practising men in Tanzania. Methods: In-depth interviews with twelve men were conducted in Dar es Salaam, 2012. Data were interpreted through qualitative content analysis. Results: Narratives revealed that men’s healthcare perceptions were shaped by previous encounters, rumours

Anaerobic digestion (AD) has gained increasing attention nowadays as an approach for both waste treatment and renewable energy generation. Currently, many different types of materials can be used as feedstock for biogas production via AD process, but their biodegradability (based on methane yield, BDCH4) and potential to produce biogas might vary significantly, and these properties are key paramet

I Emmaboda går det bra för bosnierna, bättre än i någon av Sveriges storstäder. Bosniernas yrkeserfarenhet har passat väl in i tillverkningsindustrin och i den lilla kommunen är kontakterna täta mellan arbetsförmedling och näringsliv.

Somalierna är en grupp i Sverige som redan från början betraktades som ”svårintegrerade”. Sådana epitet blir ofta självuppfyllande. I USA däremot har de rykte om sig att vara hårt arbetande, målmedvetna, djärva och flexibla.

Ett fiskbestånd syftar på en grupp artfränder som uppehåller sig inom ett avgränsat område och som huvudsakligen parar sig sinsemellan, vilket medför att de genetiska likheterna är större inom ett bestånd än mellan separata bestånd. Denna beskrivning stämmer väl in på sjölevande fiskarter, där fisk sällan kan röra sig mellan sjöar, vilket alltså leder till genetiska skillnader mellan bestånd i oliPrudent management of marine fish resources relies on separation and management of biologically meaningful groups of conspecifics, termed fishery stocks, often depicted as self-sustaining, spatiotemporally separated and demographically independent entities. Such stock discrimination has however proven challenging. Although most marine fish species form local spawning populations (in relation to hy