Four -1,4-glucanases (cellulases) of the cellulolytic bacterium Cellulomonas fimi were purified from Escherichia coli cells transformed with recombinant plasmids. Previous analyses using soluble substrates had suggested that CenA and CenC were endoglucanases while CbhA and CbhB resembled the exo-acting cellobiohydrolases produced by cellulolytic fungi. Analysis of molecular size distributions duri

We have prospectively evaluated the biologic response to desmopressin in 77 patients with type 1 von Willebrand disease (VWD) enrolled within the Molecular and Clinical Markers for the Diagnosis and Management of type 1 VWD project. Complete response to desmopressin was defined as an increase of both ristocetin cofactor activity (VWF:RCo) and factor VIII coagulant activity (FVIII:C) to 50 IU/dL or

Hexadecyltrimethylammonium bromide (C(16)TAB)-sodium perfluorooctanoate (C8FONa) and hexadecylpyridynium bromide (C16PyB)-(C8ONa catanionic semifluorinated mixtures have been studied by conductivity, dynamic light scattering (DLS), cryo-transmission electron microscopy (cryo-TEM) and polarizing microscopy. The regular Solution theory, applicable for a limited fluorinated molar ratio, does not pred

The bit-search generator (BSG) was proposed in 2004 and can be seen as a variant of the shrinking and self-shrinking generators. It has the advantage that it works at rate 1/3 using only one LFSR and some selection logic. We present various attacks on the BSG based on the fact that the output sequence can be uniquely defined by the differential of the input sequence. By knowing only a small part o

A review essay discussing the debate between Axel Honneth and Nancy Fraser on recognition and redistribution, the topic of recognition in relation to the sociology of work, and recognition-research in the Nordic countries.

The aim was to investigate if functional balance performance in patients with Parkinson's disease (PD) was affected by long-term (3 years) treatment with bilateral subthalamic nucleus (STN) high-frequency stimulation. Thirty-five patients were consecutively included, and 28 patients completed the study (mean age 62 years, SD 6.5). The Berg Balance Scale (BBS) was assessed preoperatively and 1 and

Metal ions are essential cofactors for precursor tRNA (ptRNA) processing by bacterial RNase P. The ribose 2'-OH at nucleotide (nt) –1 of ptRNAs is known to contribute to positioning of catalytic Me2+. To investigate the catalytic process, we used ptRNAs with single 2'-deoxy (2'-H), 2'-amino (2'-N), or 2'-fluoro (2'-F) modifications at the cleavage site (nt –1). 2' modifications had small (2.4–7.7-

Objective and design Chemotaxis of neutrophils from blood to the inflammation process plays an important role in development of periodontal inflammation. The novel chemokine GRO alpha, also named CXCL1, is a strong chemoattractant for neutrophils. Data on production and regulation of GRO alpha by oral fibroblasts have not previously been presented. Materials and methods GRO alpha mRNA and protein

Plasmodium falciparum invades erythrocytes through multiple ligand-receptor interactions, with redundancies in each pathway. One such alternate pathway is the trypsin-resistant pathway that enables P. falciparum to invade trypsin-treated erythrocytes. Previous studies have shown that this trypsin-resistant pathway is dependent on glycophorin B, as P. falciparum strains invade trypsin-digested glyc

High fat diets and obesity pose serious health problems, such as type II diabetes and cardiovascular disease. Impaired cognitive function is also associated with high fat intake. In this study, we show that just 4 weeks of feeding a diet rich in fat ad libitum decreased hippocampal neurogenesis in male, but not female, rats. There was no obesity, but male rats fed a diet rich in fat exhibited elev

Objective To investigate a possible association between the longitudinal changes in skin involvement and the fibroblast production of proteoglycans in vitro, among patients with early and untreated systemic sclerosis (SSc). Methods In 11 patients, 6 with diffuse cutaneous systemic sclerosis (dSSc) and 5 with limited cutaneous systemic sclerosis (lSSc), and in 6 controls skin thickness and skin ech

Huntington's disease (HD) is a hereditary neurodegenerative disorder caused by a CAG repeat expansion in the HD gene. Excitotoxic cell damage by excessive stimulation of glutamate receptors has been hypothesized to contribute to the pathogenesis of HD. Transgenic mouse models of HD have shown variable sensitivity to excitotoxicity. The models differ in the genetic background, the type and length o

Plasmacytoid dendritic cells (pDCs) are involved in innate immunity (eg, by secreting interferons) and also give rise to CD4(+)CD56(+) hematodermic neoplasms. We report extensive characterization of human pDCs in routine tissue samples, documenting the expression of 19 immunohistologic markers, including signaling molecules (eg, BLNK), transcription factors (eg, ICSBP/IRF8 and PU.1), and Toll-like

Bikunin is a Kunitz-type proteinase inhibitor, which is cross-linked to heavy chains via a chondroitin sulfate chain, forming inter-alpha-inhibitor and related molecules. Rat bikunin was produced by baculovirus-infected insect cells. The protein could be purified with a total yield of 20 mg/liter medium. Unlike naturally occuring bikunin the recombinant protein had no galactosaminoglycan chain. En

N,N'-disalicylidene-1,2-diaminopropane is a copper inhibitor present in some adhesive plasters, rubber products and gasoline. Upon contact with water it is hydrolyzed to salicylaldehyde and 1,2-diaminopropane. All patients in this study showed positive patch-test reactions to N,N'-disalicylidene-1,2-diaminopropane, and also to 1,2-diaminopropane and ethylenediamine. None reacted to salicylaldehyde

The Type 1 Diabetes Genetics Consortium (T1DGC), funded September 2002, is a collaborative effort to develop resources for the purpose of identifying risk genes for type 1 diabetes. One of the aims is to establish a renewable resource of DNA on 2500 affected sib-pair families. Regional networks have been established and in continental Europe, the European Type 1 Diabetes Genetics Network (ET1DGN)

We prepared a Au/CeO2 (111) model catalyst by depositing a thin cerium oxide film on a Ru(0001) surface and subsequently depositing gold. This model system was investigated using high-resolution photoemission spectroscopy. Gold forms metallic nanoparticles on CeO2 with an average particle size that depends on the Au dose. At 80 K adsorption of CO was observed on the supported Au particles, which i

The traditional design method for plate heat exchangers(PHEs), either epsilon-number of transfer units (epsilon-NTU) or logarithmic mean temperature difference method, involves many trials in order to meet the pressure drop constraints. This can be avoided through the developed design method, which takes the full utilization of the allowable pressure drops as a design objective. The proposed metho

Simultaneous Buda-Lund hydro model fits are presented to identified particle spectra and two-particle Bose- Einstein correlations as measured by the STAR Collaboration in root s = 200 GeV p+p collisions at RHIC. Preliminary results are compared to similar results in Au+Au collisions at RHIC, hadron+p reactions as well as Pb+Pb collisions at CERN SPS.

A wideband space-time channel model is defined, which captures the multiple dependencies and variability in multicell system-wide operating environments. The model provides a unified, treatment of spatial and temporal parameters, giving their statistical description and dependencies across a large geographical area for three outdoor environments pertinent to third-generation cellular system simula