The Lake Poopo basin, located on the Bolivian Altiplano, is extremely vulnerable to environmental degradation. The basin displays extreme spatial and temporal variations of water resources and rapidly decreasing water quality due to anthropogenic and natural pollution. The region’s population lives in extreme poverty, and the authorities’ efforts to manage the water resources efficiently have been

This study discusses subordination in Swedish from the perspective of three construction types that involve clauses that have traditionally been difficult to classify as unambiguous main or subordinate clauses: “embedded V2”-constructions, direct speech constructions, and exclamatives. A general hypothesis regarding subordination and "superordination” is proposed: The Highest Force Hypothesis. The

The thesis is about how students can learn to use argumentation and the processes of giving responses in a web-based setting, as a tool for individual and collective learning. The thesis comprises three studies, which examine and describe how 70 distance students develop an individual and collective competence to provide feedback, critically evaluate and argue in the context of a web-based learnin

Glycoproteins in cerebrospinal fluid (CSF) are altered in Alzheimer's Disease (AD) patients compared to control individuals. We have utilized albumin depletion prior to 2D gel electrophoresis to enhance glycoprotein concentration for image analysis as well as structural glycoprotein determination without glycan release using mass spectrometry (MS). The benefits of a direct glycoprotein analysis ap

The primary sequence of Erythrina cristagalli lectin (ECL) was mapped by mass spectrometry, and the crystal structures of the lectin in complex with lactose and 2'-alpha-L-fucosyllactose were determined at 1.6A and 1.7A resolution, respectively. The two complexes were compared with the crystal structure of the closely related Erythrina corallodendron lectin (ECorL) in complex with lactose, with th

Until recently, the development of vaccines for use in humans relied on the response to attenuated or whole-cell preparations, or empirically selected antigens. The post-genomic era holds the possibility of rational design of novel vaccines for important human pathogens. The discovery and development of these new vaccines is likely to be accomplished through integrated proteomic strategies. Althou

Escherichia coli is a gram-negative bacterium that causes sepsis and infections of the nervous system, and the digestive and urinary tracts. The availability of the complete nucleotide sequence encoding the E. coli K-12 genome has made this organism an excellent model for proteomic studies. Semi-preparative two-dimensional electrophoresis, including liquid phase isoelectric focusing (IEF), one-dim

Effective material parameters for diffusion and elastic deformation are calculatedfor porous materials using a continuum theory-based superposition procedure. The theory that is limited to two-dimensional cases, requires that the pores are sufficiently sparse. The methodleads to simple manual calculations that can be performed by, e.g. hospital staff at clinicaldiagnoses of bone deceases that invo

Previous studies about the trends and sources of PV soft cost reduction have only offered a limited understanding of systemic aspects that drive this development. Drawing on concepts of sectoral innovation system theory, this study analyzes the evolution of the German PV sectoral system since the early 1990s, focusing specifically on key processes associated with the deployment of building-sited P

The evolution of Earth's biota is intimately linked to the oxygenation of the oceans and atmosphere. We use the isotopic composition and concentration of molybdenum (Mo) in sedimentary rocks to explore this relationship. Our results indicate two episodes of global ocean oxygenation. The first coincides with the emergence of the Ediacaran fauna, including large, motile bilaterian animals, ca. 550-5

Burgess Shale-type biotas occur globally in the Cambrian record and offer unparalleled insight into the Cambrian explosion, the initial Phanerozoic radiation of the Metazoa. Deposits bearing exceptionally preserved soft-bodied fossils are unusually common in Cambrian strata; more than 40 are now known. The well-documented decline of soft-bodied preservation following the Middle Cambrian represents

PURPOSE: To establish neuronal connections in the rod and cone pathway between laminated rabbit retinal transplants and the host retina.METHODS: Fourteen adult rabbits received a complete full-thickness embryonic transplant. After survival times of 3 to 10 months, the retinas were studied under light microscope and with immunohistochemistry. Antibodies against protein kinase C (PKC), parvalbumin,