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Antigenicity of cystatin-binding proteins from parasitic protozoan. Detection by a proteinase inhibitor based capture immunoassay (PINC-ELISA)

A novel immunoassay (PINC-ELISA) was designed using proteinase inhibitors of the cystatin superfamily (PINC) in the solid phase, to promote the selective capture of cysteine proteinases. The method was applied in the identification of papain-like antigens from parasitic protozoa. PINC of human origin, namely recombinant cystatin C (r-cystatin C) or low molecular weight kininogen were used in the a

Genetic diversity of cutaneous human papillomaviruses

Human papillomaviruses (HPVs) of the genera Betapapillomavirus and Gammapapillomavirus are common on human skin. Sequencing of subgenomic amplicons of cutaneous HPVs has revealed a large number of novel putative HPV types within these genera. Phylogenetic analysis based on these amplicons revealed 133 putative HPV types with

Fly ash penetration through electrostatic precipitator and flue gas condenser in a 6 MW biomass fired boiler

The effects of an electrostatic precipitator and a flue gas condenser on size resolved fly ash particle concentration and composition were studied in a 6 MW biomass combustion unit, fired with moist forest residue. The boiler was of moving grate type. The fly ash particles were sampled upstream and downstream of the electrostatic precipitator and flue gas condenser, respectively. Fine particle num

Relaxation of shear-aligned wormlike micelles

We report on the first time-resolved small-angle neutron scattering (SANS) study of the structural relaxation from a shear-induced aligned state back to the isotropic state in a system of giant wormlike reverse micelles. The micelles are formed by lecithin and small amounts of water in cyclohexane. A liquid sample with the micellar volume fraction phi = 0.3 was exposed to a steady shear of (gamma)

Impact on participation and autonomy questionnaire: internal scale validity of the Swedish version for use in people with spinal cord injury.

Objective: To evaluate aspects of internal scale validity and reliability of the Swedish version of the Impact on Participation and Autonomy questionnaire (IPA-S) for use in people with spinal cord injury. Subjects: A total of 161 persons with spinal cord injury. Method. IPA-S psychometric properties were evaluated using Rasch rating scale analysis. Results: The results show that the IPA-S has 27

Onshore climate change during the late triassic marine inundation of the Central European Basin

Sedimentological, pedological and palynological evidence reveal radically increasing onshore humidity during the Rhaetian marine invasion of central Europe along the north-eastern margin of the Central European Basin (southern Sweden). Pre-Rhaetian aridity favoured the formation of Carnian redbeds with calcrete, which were succeeded by Norian hematite-cemented conglomerates, arkoses, arkosic wacke

Glycaemic index methodology

The glycaemic index (GI) concept was originally introduced to classify different sources of carbohydrate (CHO)-rich foods, usually having an energy content of > 80 % from CHO, to their effect on post-meal glycaemia. It was assumed to apply to foods that primarily deliver available CHO, causing hyperglycaemia. Low-GI foods were classified as being digested and absorbed slowly and high-GI foods as b

Cloning of alkaline sphingomyelinase from rat intestinal mucosa and adjusting of the hypothetical protein XP_221184 in GenBank.

Intestinal alkaline sphingomyelinase (alk-SMase) digests sphingomyelin and the process may influence colonic tumorigenesis and cholesterol absorption. We recently identified the gene of human alk-SMase and cloned the cDNA. Cross-species screening of homology in GenBank found a hypothetical rat protein, XP_221184, with 491 amino acid residues, which shares 73% identity with human alk-SMase. Based o

Tumor extension in high-grade gliomas assessed with diffusion magnetic resonance imaging: values and lesion-to-brain ratios of apparent diffusion coefficient and fractional anisotropy.

Purpose: To determine whether the apparent diffusion coefficient (ADC) and fractional anisotropy (FA) can distinguish tumor-infiltrated edema in gliomas from pure edema in meningiomas and metastases. Material and Methods: Thirty patients were studied: 18 WHO grade III or IV gliomas, 7 meningiomas, and 5 metastatic lesions. ADC and FA were determined from ROIs placed in peritumoral areas with T2-s

Expression of islet iNOS and inhibition of glucose stimulated insulin release after long-term lipid infusion in the rat is counteracted by PACAP27.

Chronic exposure of pancreatic islets to elevated plasma lipids ( lipotoxicity) can lead to beta-cell dysfunction, with overtime becoming irreversible. We examined, by confocal microscopy and biochemistry, whether the expression of islet inducible nitric oxide synthase ( iNOS) and the concomitant inhibition of glucose-stimulated insulin release seen after lipid infusion in rats was modulated by th

Stomatal closure by ultraviolet-radiation

The effect of ultraviolet radiation (UV) (255-325 nm) on stomatal closure was investigated on tef (Eragrostis tef (Zucc) Trotter) in the presence of white light (ca 50 .mu.mol m-2 s-1). The action spectrum showed that UV (ca 2 .mu.mol m-2 s-1, half band width about 10 nm) of 285 nm or shorter wavelengths was very efficient in causing stomatal closure. The effectiveness decreased sharply towards lo

Metoder att välja korrektioner vid fuktberäkningar med variabelt utomhusklimat

Popular Abstract in SwedishMånga skador och olägenheter av fukt kan uppkomma under en byggnads livslängd. De kan t.ex. orsaka hälsoproblem eller dålig ekonomi. För att minska fuktproblem kan en fuktdimensionering göras. I en fuktdimensionering behövs olika fuktberäkningar för att se hur fukttillståndet förväntas bli i eller på en yta av en konstruktion. Beräknade fukttillstånd används för att kontDetermination of the design conditions for moisture resistance requires in a Moisture Design number of different moisture calculations to be made in order to determine the moisture status within, or on the surface of, a structure. The objective of this work has been to ascertain how to allow for variations in climate data in order to be able to determine the most unfavourable moisture status likel