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The aim of this thesis is to investigate the functions of the word "tot" 'whole; each; all' in old and modern Romanian, by studying the syntax, morphology and semantics of this word as part of or in combination with a noun phrase (NP). An attempt is made to find out the status of "tot" in a classification of numerals, articles and adjectives. The present work consists of four separate studies (i.

Fulminating clostridial septicemia in children treated for lymphoproliferative disorders

Overwhelming Clostridium septicum infection in 2 children, 1 and 4 yr old, with acute lymphoblastic leukemia and B-cell non-Hodgkin malignant lymphoma, respectively, as well as fatal C. perfringens infection in a 3-yr-old child with histiocytosis-X are reported. A neutropenic patient with fever, abdominal symptoms and hypotension--but otherwise being well--must be suspected of having clostridial d

Naturally conceived twins with monochorionic placentation have the highest risk of fetal loss

Objective The aim of this study was to estimate the rate of fetal loss in dichorionic (DC) and monochorionic (MC) twin pregnancies stratified according to zygosity and method of conception. Methods In a prospective multicenter observational study women with a twin pregnancy bad an ultrasound scan before 14 + 6 weeks' gestation in order to determine chorionicity. The fetal loss rate, the perinatal,

Controlling Fault-Prone Components for Software Evolution

The amount of software around us has increased incredibly. A lot of the new software is integrated into different devices, for example, home electronics, cars and cell-phones. As existing and new systems evolve through different maintenance releases, they naturally inherit functionality and characteristics from previous releases and therefore become what we refer to as legacy systems. Often new fu

Sd(a)-antigen-like structures carried on core 3 are prominent features of glycans from the mucin of normal human descending colon

This paper describes structural characterization by NMR, MS and degradative studies of mucin glycans from normal human descending colon obtained freshly at autopsy. The saccharides were mainly based on core 3 (GlcNAcbeta1-3GalNAc). Among the terminal saccharide determinants Sd(a)/Cad-antigen-like structures were prominent, and Lewis x, sialyl Lewis x and sulphated Lewis x were found as minor compo

Selection of DNA aptamers against rat liver X receptors

Liver X receptors alpha and beta (LXRα; LXRβ) are members of the nuclear hormone receptor superfamily of ligand-activated transcription factors. LXRs play an important role in the reverse cholesterol transport and govern the expression of many of the proteins that are indispensable for the regulation of normal cholesterol levels in the body. SELEX, an in vitro selection technology, was used on a s

Enzyme dynamics during catalysis

Internal protein dynamics are intimately connected to enzymatic catalysis. However, enzyme motions linked to substrate turnover remain largely unknown. We have studied dynamics of an enzyme during catalysis at atomic resolution using nuclear magnetic resonance relaxation methods. During catalytic action of the enzyme cyclophilin A, we detect conformational fluctuations of the active site that occu

Cartilage-specific autoimmunity in animal models and clinical aspects in patients - focus on relapsing polychondritis

Relapsing polychondritis is an autoimmune disease in which an inappropriate immune response destroys cartilage. Cartilage of the ears, larynx and nose rather than spine and joint cartilage is affected by a chronic relapsing and erosive inflammation. Several animal models for relapsing polychondritis have been published in which immunization with various cartilage proteins induces a variety of chon

Influence of lifestyle behaviours on bone mineral density among young healthy women: A two-year study. A Tentative Salutary Model.

Popular Abstract in Swedish Skeletthälsa och livsstilsbeteenden hos unga kvinnor I Sverige kommer ungefär 50 % av alla kvinnor att drabbas av en fraktur någon gång under sitt liv. Ett starkt skelett med hög mängd benmassa är det som främst bidrar till att förebygga benskörhetsfrakturer. Kvinnor har efter ungdomsåren mindre mängd benmassa än män och mängden benmassa minskar hos kvinnor snabbare i The overall aim of this prospective observational study with a salutogenic approach was to investigate bone mineral density (BMD) and BMD changes in the heel bone, in a group of healthy women, in relation to physiological factors and lifestyle behaviours over a two-year period as well as investigating the women’s view of their lifestyle behaviours. Data were collected in 1999 (T1) and 2001 (T2). H

Microarrays based on affinity-tagged SCFV antibodies: Sensitive detection of analyte in complex proteomes

Antibody microarray is a novel technology that will allow us to efficiently perform global proteome analysis. Performing rapid and multiplexed analysis on complex proteomes, such as human serum, targeting also low-abundant analytes will, however, place high demands upon the microarray design with respect to logistics, specificity and sensitivity. In this study, we have shown that non-purified affi