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The N-terminal N5 subdomain of the alpha 3(VI) chain is important for collagen VI microfibril formation

Collagen VI assembly is unique within the collagen superfamily in that the alpha 1(VI), alpha 2(VI), and alpha 3(VI) chains associate intracellularly to form triple helical monomers, and then dimers and tetramers, which are secreted from the cell. Secreted tetramers associate end-to-end to form the distinctive extracellular microfibrils that are found in virtually all connective tissues. Although

Experimental observation of different-order components of a vibrational wave packet in a bulk dielectric using high-order Raman scattering

We use high-order Raman scattering in a bulk dielectric to characterize coherent dynamics with precision typical for gas phase experiments. The experimental pump-probe approach allows for the simultaneous observation and separation in space and time of the individual contributions of different-order Raman processes to a coherent wave packet without relying on phase-matching conditions and within t

What is the cause of the ageing atrophy? Total number, size and proportion of different fiber types studied in whole vastus lateralis muscle from 15- to 83-year-old men

In order to study the effects of increasing age on the human skeletal muscle, cross-sections (15 micron) of autopsied whole vastus lateralis muscle from 43 previously physically healthy men between 15 and 83 years of age were prepared and examined. The data obtained on muscle area, total number, size, proportion and distribution of type 1 (slow-twitch) and type 2 (fast-twitch) fibers were analysed

Prediction of tumor heterogeneity in localized prostate cancer

The clinical T1-T2 prostatic carcinoma is a heterogeneous tumor in respect to pathologic stage and outcome. Tumor heterogeneity can be fairly good predicted by the use of classification and regression tree analysis with preoperative parameters, especially a quantitative analysis of Gleason grade 4-5 cancer in six systematic biopsies and a determination of preoperative prostate-specific antigen lev

Increased expression of vasoactive intestinal polypeptide in cultured myenteric neurons from adult rat small intestine.

Adult neurons possess the ability to adapt to a changing environment. Loss of target-derived neurotrophic factors due to axotomy or isolation by culturing is known to induce changes in neuropeptide expression in several types of peripheral neurons. The aim of the present study was to investigate changes in the expression of vasoactive intestinal polypeptide (VIP) and nitric oxide synthase (NOS) in

Light-activated desorption of photoactive polyelectrolytes from supported lipid bilayers

Phospholipid vesicles and supported bilayers have emerged as a promising platform for the development of biorecognition devices. To expand the capabilities of such biochips, it becomes desirable to direct and control the assembly of lipid structures into more sophisticated architectures. As one step toward this goal, we demonstrate the photoregulated desorption of a new class of polymer from lipid

Degradation of phenol and cresols at low temperatures using a suspended-carrier biofilm process

Degradation of phenol and o-, m- and p-cresol at a concentration of 150 mg; l(-1) of each compound was studied in a suspended-carrier biofilm process consisting of two aerobic stages. The fungus Mortierella sarnyensis Mil'ko dominated the microflora. in the first reactor, while bacteria dominated in the second reactor. The process was studied at 4, 7, 11 and 15 degreesC. The results from the exper

Assessing the role of different spatial frequencies in word perception by good and poor readers

Numerous studies indicate that dyslexic and nondyslexic individuals exhibit different patterns of sensitivity to spatial frequency. However, the extension of this effect to normal (nondyslexic) adults of good and poor reading abilities and the role played by different spatial frequencies in word perception have yet to be determined. In this study, using normal (nondyslexic) adults, we assessed rea

Quantum memory for nonstationary light fields based on controlled reversible inhomogeneous broadening

We propose a method for efficient storage and recall of arbitrary nonstationary light fields, such as, for instance, single photon time-bin qubits or intense fields, in optically dense atomic ensembles. Our approach to quantum memory is based on controlled, reversible, inhomogeneous broadening and relies on a hidden time-reversal symmetry of the optical Bloch equations describing the propagation o

Effect of casein concentration in suspensions and gels on poly(ethylene glycol)s NMR self-diffusion measurements

PFG-NMR spectroscopy was used to study the diffusion of molecular probes (poly(ethylene glycol)s) in casein suspensions and gels in terms of the effects of probe molecular size (molecular mass between 1080 and 634 000 g/mol), casein concentrations (from 3.24 to 16.22 g/100 g), and effects of rennet coagulation. A strong dependency of diffusion on probe size was observed, both in casein suspensions

The influence of patent foramen ovale on oxygen desaturation in obstructive sleep apnoea

Obstructive sleep apnoea (OSA) is associated with oxygen desaturation to a varying degree. A patent foramen ovale (PFO) may allow interatrial right-to-left shunting. The hypothesis of the current study was that oxygen desaturation will occur more often, in proportion to the frequency of respiratory disturbances, in OSA subjects with PFO than in those without. In a group of 209 subjects diagnosed w

Community education for cardiovascular disease prevention. Morbidity and mortality results from the Minnesota Heart Health Program

The Minnesota Heart Health Program was a community trial of cardiovascular disease prevention methods that was conducted from 1980 to 1990 in three Upper Midwestern communities with three matched comparison communities. A 5- to 6-year intervention program used community-wide and individual health education in an attempt to decrease population risk. A major hypothesis was that the incidence of vali

Early Eocene climatic, volcanic, and biotic events in the northwestern Tethyan Untersberg section, Austria

The 40 in thick Untersberg section (Salzburg, Austria) of the Northern Calcareous Alps comprises the Palaeocene-Eocene transition and spans the upper part of calcareous nannoplankton zone NP9 and the lower part of zone NP10 (subzone NP10a). These zones are equivalent to planktonic foraminifera zone P5 and the lower part of zone P6 (sub-zone P6a). The succession was deposited in a lower bathyal slo

Identification of various exon combinations of the ews/fli1 translocation: An optimized RT-PCR method for paraffin embedded tissue a report by the CWS-Study group - A report by the CWS-study group

Background: Chromosomal translocations t(11;22) (q24;q12) are characteristic of about 80-90% of Ewing's sarcoma family of tumors [bone and soft tissue Ewing's sarcoma and peripheral neuroectodermal tumors (PNET)]. They generate ews/fli1 rearrangements showing great diversity in breakpoint exon combination. In about 5% of Ewing's tumors, ews is fused to the erg gene at 21q22. The various chimeric p

Fetal growth and onset of delivery: A nationwide population-based study of preterm infants

Objective: This study was undertaken to assess whether deviations from normal fetal growth are associated with spontaneous preterm delivery. Study design: A population-based study was performed, using Swedish Medical Birth Register data from 1991 through 2001. The total population comprised 1,007,648 singleton births. Intrauterine-derived growth standards were used to identify individual standard

Concomitant contact dermatitis due to textile dyes and to colour film developers can be explained by the formation of the same hapten

p-phenylenediamine derivatives are widely used in industry and in cosmetics, and several of them are well-known sensitizers. One group of allergenic p-phenylenediamine derivatives are used as colour film developers. Cross-reactivity between the colour film developers has been reported. In this paper, an occupational facial dermatitis due to colour film developers is described. The patient reacted

Primary prophylaxis in severe haemophilia should be started at an early age but can be individualized

The frequency of joint bleeds and orthopaedic joint scores were evaluated in 121 patients with severe haemophilia who had started prophylactic treatment with clotting factor concentrates at least once weekly before the age of 10. 75 of the patients started before the age of 3, 31 at the age of 3-5 and 15 at the age of 6-9. Each subgroup was evaluated separately. In addition, a regimen of one infus