Attached glial-like cell cultures were established from the lateral and medial ganglionic eminences (LGE and MGE) and from the neocortex (Cx) of E13.5 mouse embryos, and expanded over four to five passages under epidermal growth factor (EGF) stimulation. Following removal of EGF and serum, we analysed the generation of neurons and glial cells within the cultures. Significant numbers of βIII-tubuli

Background: Computed tomography (CT) lung densitometry has been demonstrated to be the most sensitive and specific outcome measure for the assessment of emphysema-modifying therapy, but the optimum densitometric index has yet to be determined and targeted sampling may be more sensitive than whole lung assessment. The EXAcerbations and CT scan as Lung Endpoints (EXACTLE) trial aimed to clarify the

Human papillomavirus (HPV) DNA testing can be used to identify women at risk of the development of cervical cancer. The cost-effectiveness of HPV screening is dependent on the type-specific HPV prevalence in the general population. The present study describes the prevalence and spectrum of high-risk HPV types found in a large real-life population-based HPV screening trial undertaken entirely withi

To determine which nuclei may exhibit shape isomerism, we use a well-benchmarked macroscopic-microscopic model to calculate potential-energy surfaces as functions of spheroidal (epsilon(2)), hexadecapole (epsilon(4)), and axial-asymmetry (gamma) shape coordinates for 7206 nuclei from A=31 to A=290. We analyze these and identify the deformations and energies of all minima deeper than 0.2 MeV. These

Wetlands are significant sources of the important greenhouse gas CH4. Here we explore the use of an experimental system developed for the determination of continuous fluxes of CO2 and CH4 in closed ecosystem monoliths including the capture of (CO2)-C-14 and (CH4)-C-14 following pulse labelling with (CO2)-C-14. We show that, in the ecosystem studied, ebullition (bubble emission) may account for 18

Background Optimal treatment of acute myocardial infarction (AMI) depends on the duration of the ischemia. The Anderson Wilkins (AW) electrocardiographic acuteness score has been shown to complement the historical timing in estimating the time interval from acute thrombotic coronary occlusion in patients presenting with chest pain and evolving myocardial infarction. The purposes of this study were

Total fungal biomass, the biomass of ectomycorrhizal and ericoid mycorrhizal (EM + ErM), and arbuscular mycorrhizal (AM) fungi, as well as the production of EM and AM fungi, were estimated in coniferous forest soils along four natural nutrient gradients. Plant community changes, forest productivity, soil pH and N availability increase over relatively short distances (< 100 m) along the gradients.

Objective: To validate self-reported information on weight and height in an adult population and to find a useful algorithm to assess the prevalence of obesity based on self-reported information. Research Methods and Procedures: This was a crosssectional survey consisting of 1703 participants (860 men and 843 women, 30 to 75 years old) conducted in the community of Vara, Sweden, from 2001 to 2003.

he aim of this study was to scrutinize individual items as well as total scores of the Suicidal Intent Scale (SIS) in order to find out its usefulness for predicting suicide. Consecutive patients (N=555) were evaluated and rated with SIS shortly after a suicide attempt. When followed up at a mean time of 4.5 years, 22 (4%) had committed suicide. Those who later committed suicide had been scored si

Free neutral chloromethane clusters have been produced by adiabatic expansion and investigated by means of photoelectron spectroscopy. By studying the differences between the cluster binding energy shifts at the Cl 2p and C 1s thresholds we have shown that antiparallel packing is the dominant local structure of the clusters. This geometry is induced by the polar character of the constituent molecu

In this article, the competitive adsorption of egg yolk proteins at oil/water interfaces during emulsification is studied. By using two-dimensional polyacrylamide electrophoresis and mass spectrometry, it was possible to characterize and identify adsorbing and non-adsorbing protein species. The egg yolk contains proteins with a wide range of molecular weights and pI. Lipoproteins adsorbed selectiv

The clinical feature in patients with congenital factor VII deficiency is in part dependent on the underlying genetic defect, but the mechanisms influencing the genotype-phenotype correlation remain to be fully elucidated. In addition, thromboembolic events have been reported. Compensatory mechanisms involving vitamin K-dependent factors have been suggested. We have measured anticoagulant activiti

The formation of SCFA in rats fed fermented oat fibre concentrates was compared with that of rats fed native oat fibre concentrate. The cultures used were lactic acid bacteria consisting of Lactobacillus bulgaricus and Streptococcus thermophilus (V2), the exopolysaccharide-producing strain Pediococcus damnosus 2.6 (Pd) and L. reuteri (Lr). The materials were incorporated into test diets yielding a

A controlled method of manipulation of nanowires was found using the tip of an Atomic Force Microscope (AFM). Manipulation is done in the 'Retrace Lift' mode, where feedback is turned off for the reverse scan and the tip follows a nominal path. The effective manipulation force during the reverse scan can be changed by varying an offset in the height of the tip over the surface. Using this method,