Search results

Filter

Filetype

Your search for "*" yielded 528652 hits

Designing robust preparative purification processes with high performance

In the production of pharmaceutical drugs, a large part of the production costs stem from the downstream processing and the chromatographic purifications required. In order to reduce purification costs the process performance must be increased, which means that the processes need to be less robust since robustness comes with the price of lower process performance, and thus, higher production costs

Lack of respiratory chain Complex I impairs alternative oxidase engagement and modulates redox signaling during elicitor-induced cell death in tobacco.

Alternative oxidase (AOX) functions in stress resistance by preventing accumulation of reactive oxygen species (ROS), but little is known about in vivo partitioning of electron flow between AOX and the cytochrome pathway. We investigated the relationships between AOX expression and in vivo activity in Nicotiana sylvestris and the complex I–deficient CMSII mutant in response to a cell death elicito

The nodal, the new and the old maximum of the Perseid meteor stream

Visual observations of Perseid meteors carried out in 1953-83 at the Onsala Space Observatory are analyzed to study the fine structure of the Perseid meteor stream maximum. Observed hourly rates are corrected for weather conditions and to a zenithal value. The Perseid activity curve is studied (1) for all observed Perseids, (2) for Perseids of app. magnitude 2.0 or brighter. The activity curve for

Folding free energies of 5'-UTRs impact post-transcriptional regulation on a genomic scale in yeast

Using high-throughput technologies, abundances and other features of genes and proteins have been measured on a genome-wide scale in Saccharomyces cerevisiae. In contrast, secondary structure in 5'-untranslated regions ( UTRs) of mRNA has only been investigated for a limited number of genes. Here, the aim is to study genome-wide regulatory effects of mRNA 5'-UTR folding free energies. We performed

History of the reindeer (Rangifer tarandus) in the eastern Baltic region and its implications for the origin and immigration routes of the recent northern European wild reindeer populations

A total of 45 subfossil reindeer (Rangifer tarandus) antlers and bones - artefacts excluded - have been found over the years in the Baltic countries of Estonia, Latvia and Lithuania. The relatively high number of specimens suggests a stable residence of the species in the eastern Baltic region. For the first time, 12 of these finds were radiocarbon-dated. The ages of the samples range between 1208

2-deoxy-2-[F-18]fluoro-D-glucose uptake and correlation to intratumoral heterogeneity

The aim of this study was to investigate the pattern of 2-deoxy-2-[F-18]fluoro-D-glucose (FDG) uptake in relation to the intratumoral histopathological appearance. Materials and Methods: Intratumoral distribution of FDG in nude mice with xenografted tumours originating from an established head and neck squamous cell carcinoma was studied. FDG uptake and the con-elation to histopathological appeara

Risk comparisons of premium rules: optimality and a life insurance study

Consider a risk Y-1 (x) depending on an observable covariate x which is the outcome of a random variable A with a known distribution, and consider a premium p(x) of the form p(x) = EY1 (x) + etap(1) (x). The corresponding adjustment coefficient gamma is the solution of E exp{gamma[Y-1(A) - p(A)]} = 1, and we characterize the rule for the loading premium p(1)((.)) which maximizes gamma subject to t

Additive effects of the major risk alleles of IRF5 and STAT4 in primary Sjogren's syndrome

Primary Sjogren's syndrome (SS) shares many features with systemic lupus erythematosus (SLE). Here we investigated the association of the three major polymorphisms in IRF5 and STAT4 found to be associated with SLE, in patients from Sweden and Norway with primary SS. These polymorphisms are a 5-bp CGGGG indel in the promoter of IRF5, the single nucleotide polymorphism (SNP) rs10488631 downstream of

Cyclic nucleotide phosphodiesterases (PDE) 3 and 4 in normal, malignant, and HTLV-I transformed human lymphocytes

Intracellular cyclic AMP, determined in part by cyclic nucleotide phosphodiesterases (PDEs), regulates proliferation and immune functions in lymphoid cells. Total PDE, PDE3, and PDE4 activities were measured in phytohemagglutinin (PHA)-activated peripheral blood mononuclear cells (PBMC-PHA), normal natural killer (NK) cells, Jurkat and Kit225-K6 leukemic T-cells, T-cell lines transformed with huma

The search for a peptide ligand targeting the lipolytic enzyme cutinase

A constrained nonapeptide phage display library was evaluated as a potential source of affinity ligand(s) for purification of cutinase, a lipolytic enzyme. After seven cycles of biopanning, 500 clones were isolated and individually tested for their capability to interact with cutinase. Three out of six sequenced clones carrying a cutinase-specific constrained peptide showed the same insert sequenc

Experience with the new genetic variant of Chlamydia trachomatis in Orebro county, Sweden - proportion, characteristics and effective diagnostic solution in an emergent situation.

A Chlamydia trachomatis variant that contains a 377 bp deletion in the cryptic plasmid was recently reported in Sweden. This deletion includes the targets for Cobas Amplicor, Cobas TaqMan48, and Abbott m2000. We examined the proportion and characteristics of this variant in Örebro county, Sweden and developed an effective diagnostic solution. In total, 2,401 consecutive C. trachomatis culture samp

Fracture mechanics in timber engineering - Strength analyses of components and joints

Traditional timber engineering design approaches use for simple geometries stress- or strain-based criteria for strength analyses. For more complex cases purely empirical methods are also used. Although practical, purely empirical methods are not general, and could lead to estimations on the unsafe side, if extrapolated outside their original scope. It is furthermore known that stress- or strain-b

Density functional theories of surface interactions in salt solutions

Most current density functional theories rely upon the presence of hard cores, also between ions of like charge. These hard cores should in principle reflect exchange repulsion. However, by the way in which these theories are formulated, the hard cores effectively determine the range of ion-ion correlations. This is because the mutual repulsion between like-charged ions is truncated below the corr

Small local population sizes and high habitat patch fidelity in a specialised solitary bee.

Andrena hattorfiana is a rare solitary bee which has declined during the last decades throughout western Europe. It is specialised to forage pollen from plants of the family Dipsacaceae. Knowledge of distribution, dispersal propensity, and local population sizes is essential for successful conservation of A. hattorfiana. The investigated local bee populations (n = 78) were dominated by small local

Immunization of Rats with Homologous Type XI Collagen Leads to Chronic and Relapsing Arthritis with Different Genetics and Joint Pathology Than Arthritis Induced with Homologous Type II Collagen.

The most commonly used animal model for rheumatoid arthritis (RA) is collagen-induced arthritis (CIA), induced by immunization with type II collagen (CII), a cartilage restricted protein. In this work we show that type XI collagen (CXI), which is a minor component in cartilage, induces a different form of erosive and chronic relapsing polyarthritis in rats. Using a series of inbred rat strains inv

Evidence-based medicine in HBP surgery: Is there any?

Background. Evidence-based medicine (EBM) has become widely accepted as a basis for clinical decision in many fields of medicine. This review examines the specific role of EBM in hepato-biliary and pancreatic (HBP) surgery. EBM relies on four main sources, including clinical guidelines, meta-analyses, primary information and clinical experience. Randomized controlled trials (RCTs) constitute the c